Combinatorial multimer staining and spectral flow cytometry facilitate quantification and characterization of polysaccharide-specific B cell immunity

Bacterial capsular polysaccharides are important vaccine immunogens. However, the study of polysaccharide-specific immune responses has been hindered by technical restrictions. Here, we developed and validated a high-throughput method to analyse antigen-specific B cells using combinatorial staining...

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Detalles Bibliográficos
Autores principales: Hoving, Dennis, Marques, Alexandre H. C., Huisman, Wesley, Nosoh, Beckley A., de Kroon, Alicia C., van Hengel, Oscar R. J., Wu, Bing-Ru, Steenbergen, Rosanne A. M., van Helden, Pauline M., Urban, Britta C., Dhar, Nisha, Ferreira, Daniela M., Kwatra, Gaurav, Hokke, Cornelis H., Jochems, Simon P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10613281/
https://www.ncbi.nlm.nih.gov/pubmed/37898698
http://dx.doi.org/10.1038/s42003-023-05444-3

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