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Absolute oxygen-guided radiation therapy improves tumor control in three preclinical tumor models
BACKGROUND: Clinical attempts to find benefit from specifically targeting and boosting resistant hypoxic tumor subvolumes have been promising but inconclusive. While a first preclinical murine tumor type showed significant improved control with hypoxic tumor boosts, a more thorough investigation of...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10613495/ https://www.ncbi.nlm.nih.gov/pubmed/37904839 http://dx.doi.org/10.3389/fmed.2023.1269689 |
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author | Gertsenshteyn, Inna Epel, Boris Giurcanu, Mihai Barth, Eugene Lukens, John Hall, Kayla Martinez, Jenipher Flores Grana, Mellissa Maggio, Matthew Miller, Richard C. Sundramoorthy, Subramanian V. Krzykawska-Serda, Martyna Pearson, Erik Aydogan, Bulent Weichselbaum, Ralph R. Tormyshev, Victor M. Kotecha, Mrignayani Halpern, Howard J. |
author_facet | Gertsenshteyn, Inna Epel, Boris Giurcanu, Mihai Barth, Eugene Lukens, John Hall, Kayla Martinez, Jenipher Flores Grana, Mellissa Maggio, Matthew Miller, Richard C. Sundramoorthy, Subramanian V. Krzykawska-Serda, Martyna Pearson, Erik Aydogan, Bulent Weichselbaum, Ralph R. Tormyshev, Victor M. Kotecha, Mrignayani Halpern, Howard J. |
author_sort | Gertsenshteyn, Inna |
collection | PubMed |
description | BACKGROUND: Clinical attempts to find benefit from specifically targeting and boosting resistant hypoxic tumor subvolumes have been promising but inconclusive. While a first preclinical murine tumor type showed significant improved control with hypoxic tumor boosts, a more thorough investigation of efficacy from boosting hypoxic subvolumes defined by electron paramagnetic resonance oxygen imaging (EPROI) is necessary. The present study confirms improved hypoxic tumor control results in three different tumor types using a clonogenic assay and explores potential confounding experimental conditions. MATERIALS AND METHODS: Three murine tumor models were used for multi-modal imaging and radiotherapy: MCa-4 mammary adenocarcinomas, SCC7 squamous cell carcinomas, and FSa fibrosarcomas. Registered T2-weighted MRI tumor boundaries, hypoxia defined by EPROI as pO(2) ≤ 10 mmHg, and X-RAD 225Cx CT boost boundaries were obtained for all animals. 13 Gy boosts were directed to hypoxic or equal-integral-volume oxygenated tumor regions and monitored for regrowth. Kaplan–Meier survival analysis was used to assess local tumor control probability (LTCP). The Cox proportional hazards model was used to assess the hazard ratio of tumor progression of Hypoxic Boost vs. Oxygenated Boost for each tumor type controlling for experimental confounding variables such as EPROI radiofrequency, tumor volume, hypoxic fraction, and delay between imaging and radiation treatment. RESULTS: An overall significant increase in LTCP from Hypoxia Boost vs. Oxygenated Boost treatments was observed in the full group of three tumor types (p < 0.0001). The effects of tumor volume and hypoxic fraction on LTCP were dependent on tumor type. The delay between imaging and boost treatments did not have a significant effect on LTCP for all tumor types. CONCLUSION: This study confirms that EPROI locates resistant tumor hypoxic regions for radiation boost, increasing clonogenic LTCP, with potential enhanced therapeutic index in three tumor types. Preclinical absolute EPROI may provide correction for clinical hypoxia images using additional clinical physiologic MRI. |
format | Online Article Text |
id | pubmed-10613495 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-106134952023-10-30 Absolute oxygen-guided radiation therapy improves tumor control in three preclinical tumor models Gertsenshteyn, Inna Epel, Boris Giurcanu, Mihai Barth, Eugene Lukens, John Hall, Kayla Martinez, Jenipher Flores Grana, Mellissa Maggio, Matthew Miller, Richard C. Sundramoorthy, Subramanian V. Krzykawska-Serda, Martyna Pearson, Erik Aydogan, Bulent Weichselbaum, Ralph R. Tormyshev, Victor M. Kotecha, Mrignayani Halpern, Howard J. Front Med (Lausanne) Medicine BACKGROUND: Clinical attempts to find benefit from specifically targeting and boosting resistant hypoxic tumor subvolumes have been promising but inconclusive. While a first preclinical murine tumor type showed significant improved control with hypoxic tumor boosts, a more thorough investigation of efficacy from boosting hypoxic subvolumes defined by electron paramagnetic resonance oxygen imaging (EPROI) is necessary. The present study confirms improved hypoxic tumor control results in three different tumor types using a clonogenic assay and explores potential confounding experimental conditions. MATERIALS AND METHODS: Three murine tumor models were used for multi-modal imaging and radiotherapy: MCa-4 mammary adenocarcinomas, SCC7 squamous cell carcinomas, and FSa fibrosarcomas. Registered T2-weighted MRI tumor boundaries, hypoxia defined by EPROI as pO(2) ≤ 10 mmHg, and X-RAD 225Cx CT boost boundaries were obtained for all animals. 13 Gy boosts were directed to hypoxic or equal-integral-volume oxygenated tumor regions and monitored for regrowth. Kaplan–Meier survival analysis was used to assess local tumor control probability (LTCP). The Cox proportional hazards model was used to assess the hazard ratio of tumor progression of Hypoxic Boost vs. Oxygenated Boost for each tumor type controlling for experimental confounding variables such as EPROI radiofrequency, tumor volume, hypoxic fraction, and delay between imaging and radiation treatment. RESULTS: An overall significant increase in LTCP from Hypoxia Boost vs. Oxygenated Boost treatments was observed in the full group of three tumor types (p < 0.0001). The effects of tumor volume and hypoxic fraction on LTCP were dependent on tumor type. The delay between imaging and boost treatments did not have a significant effect on LTCP for all tumor types. CONCLUSION: This study confirms that EPROI locates resistant tumor hypoxic regions for radiation boost, increasing clonogenic LTCP, with potential enhanced therapeutic index in three tumor types. Preclinical absolute EPROI may provide correction for clinical hypoxia images using additional clinical physiologic MRI. Frontiers Media S.A. 2023-10-12 /pmc/articles/PMC10613495/ /pubmed/37904839 http://dx.doi.org/10.3389/fmed.2023.1269689 Text en Copyright © 2023 Gertsenshteyn, Epel, Giurcanu, Barth, Lukens, Hall, Martinez, Grana, Maggio, Miller, Sundramoorthy, Krzykawska-Serda, Pearson, Aydogan, Weichselbaum, Tormyshev, Kotecha and Halpern. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Medicine Gertsenshteyn, Inna Epel, Boris Giurcanu, Mihai Barth, Eugene Lukens, John Hall, Kayla Martinez, Jenipher Flores Grana, Mellissa Maggio, Matthew Miller, Richard C. Sundramoorthy, Subramanian V. Krzykawska-Serda, Martyna Pearson, Erik Aydogan, Bulent Weichselbaum, Ralph R. Tormyshev, Victor M. Kotecha, Mrignayani Halpern, Howard J. Absolute oxygen-guided radiation therapy improves tumor control in three preclinical tumor models |
title | Absolute oxygen-guided radiation therapy improves tumor control in three preclinical tumor models |
title_full | Absolute oxygen-guided radiation therapy improves tumor control in three preclinical tumor models |
title_fullStr | Absolute oxygen-guided radiation therapy improves tumor control in three preclinical tumor models |
title_full_unstemmed | Absolute oxygen-guided radiation therapy improves tumor control in three preclinical tumor models |
title_short | Absolute oxygen-guided radiation therapy improves tumor control in three preclinical tumor models |
title_sort | absolute oxygen-guided radiation therapy improves tumor control in three preclinical tumor models |
topic | Medicine |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10613495/ https://www.ncbi.nlm.nih.gov/pubmed/37904839 http://dx.doi.org/10.3389/fmed.2023.1269689 |
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