Cargando…

Sweroside plays a role in mitigating high glucose-induced damage in human renal tubular epithelial HK-2 cells by regulating the SIRT1/NF-κB signaling pathway

Sweroside is a natural monoterpene derived from Swertia pseudochinensis Hara. Recently, studies have shown that sweroside exhibits a variety of biological activities, such as anti-inflammatory, antioxidant, and hypoglycemic effects. However, its role and mechanisms in high glucose (HG)-induced renal...

Descripción completa

Detalles Bibliográficos
Autores principales: Ma, Xiaodan, Guo, Zhixin, Zhao, Wenhua, Chen, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Physiological Society and The Korean Society of Pharmacology 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10613573/
https://www.ncbi.nlm.nih.gov/pubmed/37884285
http://dx.doi.org/10.4196/kjpp.2023.27.6.533
_version_ 1785128859469873152
author Ma, Xiaodan
Guo, Zhixin
Zhao, Wenhua
Chen, Li
author_facet Ma, Xiaodan
Guo, Zhixin
Zhao, Wenhua
Chen, Li
author_sort Ma, Xiaodan
collection PubMed
description Sweroside is a natural monoterpene derived from Swertia pseudochinensis Hara. Recently, studies have shown that sweroside exhibits a variety of biological activities, such as anti-inflammatory, antioxidant, and hypoglycemic effects. However, its role and mechanisms in high glucose (HG)-induced renal injury remain unclear. Herein, we established a renal injury model in vitro by inducing human renal tubular epithelial cell (HK-2 cells) injury by HG. Then, the effects of sweroside on HK-2 cell activity, inflammation, reactive oxygen species (ROS) production, and epithelial mesenchymal transition (EMT) were observed. As a result, sweroside treatment ameliorated the viability, inhibited the secretion of inflammatory cytokines (TNF-α, IL-1β, and VCAM-1), reduced the generation of ROS, and inhibited EMT in HK-2 cells. Moreover, the protein expression of SIRT1 was increased and the acetylation of p65 NF-kB was decreased in HK-2 cells with sweroside treatment. More importantly, EX527, an inhibitor of SIRT1, that inactivated SIRT1, abolished the improvement effects of sweroside on HK-2 cells. Our findings suggested that sweroside may mitigate HG-caused injury in HK-2 cells by promoting SIRT1-mediated deacetylation of p65 NF-kB.
format Online
Article
Text
id pubmed-10613573
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher The Korean Physiological Society and The Korean Society of Pharmacology
record_format MEDLINE/PubMed
spelling pubmed-106135732023-11-01 Sweroside plays a role in mitigating high glucose-induced damage in human renal tubular epithelial HK-2 cells by regulating the SIRT1/NF-κB signaling pathway Ma, Xiaodan Guo, Zhixin Zhao, Wenhua Chen, Li Korean J Physiol Pharmacol Original Article Sweroside is a natural monoterpene derived from Swertia pseudochinensis Hara. Recently, studies have shown that sweroside exhibits a variety of biological activities, such as anti-inflammatory, antioxidant, and hypoglycemic effects. However, its role and mechanisms in high glucose (HG)-induced renal injury remain unclear. Herein, we established a renal injury model in vitro by inducing human renal tubular epithelial cell (HK-2 cells) injury by HG. Then, the effects of sweroside on HK-2 cell activity, inflammation, reactive oxygen species (ROS) production, and epithelial mesenchymal transition (EMT) were observed. As a result, sweroside treatment ameliorated the viability, inhibited the secretion of inflammatory cytokines (TNF-α, IL-1β, and VCAM-1), reduced the generation of ROS, and inhibited EMT in HK-2 cells. Moreover, the protein expression of SIRT1 was increased and the acetylation of p65 NF-kB was decreased in HK-2 cells with sweroside treatment. More importantly, EX527, an inhibitor of SIRT1, that inactivated SIRT1, abolished the improvement effects of sweroside on HK-2 cells. Our findings suggested that sweroside may mitigate HG-caused injury in HK-2 cells by promoting SIRT1-mediated deacetylation of p65 NF-kB. The Korean Physiological Society and The Korean Society of Pharmacology 2023-11-01 2023-11-01 /pmc/articles/PMC10613573/ /pubmed/37884285 http://dx.doi.org/10.4196/kjpp.2023.27.6.533 Text en Copyright © Korean J Physiol Pharmacol https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Ma, Xiaodan
Guo, Zhixin
Zhao, Wenhua
Chen, Li
Sweroside plays a role in mitigating high glucose-induced damage in human renal tubular epithelial HK-2 cells by regulating the SIRT1/NF-κB signaling pathway
title Sweroside plays a role in mitigating high glucose-induced damage in human renal tubular epithelial HK-2 cells by regulating the SIRT1/NF-κB signaling pathway
title_full Sweroside plays a role in mitigating high glucose-induced damage in human renal tubular epithelial HK-2 cells by regulating the SIRT1/NF-κB signaling pathway
title_fullStr Sweroside plays a role in mitigating high glucose-induced damage in human renal tubular epithelial HK-2 cells by regulating the SIRT1/NF-κB signaling pathway
title_full_unstemmed Sweroside plays a role in mitigating high glucose-induced damage in human renal tubular epithelial HK-2 cells by regulating the SIRT1/NF-κB signaling pathway
title_short Sweroside plays a role in mitigating high glucose-induced damage in human renal tubular epithelial HK-2 cells by regulating the SIRT1/NF-κB signaling pathway
title_sort sweroside plays a role in mitigating high glucose-induced damage in human renal tubular epithelial hk-2 cells by regulating the sirt1/nf-κb signaling pathway
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10613573/
https://www.ncbi.nlm.nih.gov/pubmed/37884285
http://dx.doi.org/10.4196/kjpp.2023.27.6.533
work_keys_str_mv AT maxiaodan swerosideplaysaroleinmitigatinghighglucoseinduceddamageinhumanrenaltubularepithelialhk2cellsbyregulatingthesirt1nfkbsignalingpathway
AT guozhixin swerosideplaysaroleinmitigatinghighglucoseinduceddamageinhumanrenaltubularepithelialhk2cellsbyregulatingthesirt1nfkbsignalingpathway
AT zhaowenhua swerosideplaysaroleinmitigatinghighglucoseinduceddamageinhumanrenaltubularepithelialhk2cellsbyregulatingthesirt1nfkbsignalingpathway
AT chenli swerosideplaysaroleinmitigatinghighglucoseinduceddamageinhumanrenaltubularepithelialhk2cellsbyregulatingthesirt1nfkbsignalingpathway