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Higher abundance of Campylobacter in the oral microbiome of Japanese patients with moyamoya disease

Genetic factors alone cannot explain the pathophysiology of moyamoya disease (MMD), and environmental factors such as an immune response are thought to be involved. Oral and gut microbiomes have attracted attention as environmental factors in the pathophysiology of some vascular and autoimmune disea...

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Autores principales: Takayanagi, Kai, Kanamori, Fumiaki, Ishii, Kazuki, Yokoyama, Kinya, Araki, Yoshio, Sumitomo, Masaki, Maeda, Sachi, Goto, Shunsaku, Ota, Shinji, Nagata, Yuichi, Nishihori, Masahiro, Maesawa, Satoshi, Izumi, Takashi, Takasu, Syuntaro, Saito, Ryuta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10613609/
https://www.ncbi.nlm.nih.gov/pubmed/37899472
http://dx.doi.org/10.1038/s41598-023-45755-3
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author Takayanagi, Kai
Kanamori, Fumiaki
Ishii, Kazuki
Yokoyama, Kinya
Araki, Yoshio
Sumitomo, Masaki
Maeda, Sachi
Goto, Shunsaku
Ota, Shinji
Nagata, Yuichi
Nishihori, Masahiro
Maesawa, Satoshi
Izumi, Takashi
Takasu, Syuntaro
Saito, Ryuta
author_facet Takayanagi, Kai
Kanamori, Fumiaki
Ishii, Kazuki
Yokoyama, Kinya
Araki, Yoshio
Sumitomo, Masaki
Maeda, Sachi
Goto, Shunsaku
Ota, Shinji
Nagata, Yuichi
Nishihori, Masahiro
Maesawa, Satoshi
Izumi, Takashi
Takasu, Syuntaro
Saito, Ryuta
author_sort Takayanagi, Kai
collection PubMed
description Genetic factors alone cannot explain the pathophysiology of moyamoya disease (MMD), and environmental factors such as an immune response are thought to be involved. Oral and gut microbiomes have attracted attention as environmental factors in the pathophysiology of some vascular and autoimmune diseases. However, the relationship between MMD and these microbiomes is yet to be thoroughly investigated. This prospective case–control study aimed to compare the microbiomes of Japanese patients with MMD with those of healthy individuals to identify the specific bacteria involved in MMD. Saliva and fecal samples were collected from 16 patients with MMD who had not undergone revascularization surgery. Fifteen healthy individuals were matched for age, sex, and body mass index. The microbiomes were determined using 16S rRNA sequencing and analyzed using QIIME2. Differentially abundant microbes were identified using LEfSE and ANCOM-BC. In the oral microbiome, the two analytical methods showed that Campylobacter was more abundant in patients with MMD than in healthy individuals. Differences in the gut microbiome were smaller than those in the oral microbiome. In conclusion, the oral microbiome profiles of patients with MMD significantly differ from those of healthy individuals. Campylobacter spp. could be a substantial environmental factor in the pathophysiology of MMD.
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spelling pubmed-106136092023-10-31 Higher abundance of Campylobacter in the oral microbiome of Japanese patients with moyamoya disease Takayanagi, Kai Kanamori, Fumiaki Ishii, Kazuki Yokoyama, Kinya Araki, Yoshio Sumitomo, Masaki Maeda, Sachi Goto, Shunsaku Ota, Shinji Nagata, Yuichi Nishihori, Masahiro Maesawa, Satoshi Izumi, Takashi Takasu, Syuntaro Saito, Ryuta Sci Rep Article Genetic factors alone cannot explain the pathophysiology of moyamoya disease (MMD), and environmental factors such as an immune response are thought to be involved. Oral and gut microbiomes have attracted attention as environmental factors in the pathophysiology of some vascular and autoimmune diseases. However, the relationship between MMD and these microbiomes is yet to be thoroughly investigated. This prospective case–control study aimed to compare the microbiomes of Japanese patients with MMD with those of healthy individuals to identify the specific bacteria involved in MMD. Saliva and fecal samples were collected from 16 patients with MMD who had not undergone revascularization surgery. Fifteen healthy individuals were matched for age, sex, and body mass index. The microbiomes were determined using 16S rRNA sequencing and analyzed using QIIME2. Differentially abundant microbes were identified using LEfSE and ANCOM-BC. In the oral microbiome, the two analytical methods showed that Campylobacter was more abundant in patients with MMD than in healthy individuals. Differences in the gut microbiome were smaller than those in the oral microbiome. In conclusion, the oral microbiome profiles of patients with MMD significantly differ from those of healthy individuals. Campylobacter spp. could be a substantial environmental factor in the pathophysiology of MMD. Nature Publishing Group UK 2023-10-29 /pmc/articles/PMC10613609/ /pubmed/37899472 http://dx.doi.org/10.1038/s41598-023-45755-3 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Takayanagi, Kai
Kanamori, Fumiaki
Ishii, Kazuki
Yokoyama, Kinya
Araki, Yoshio
Sumitomo, Masaki
Maeda, Sachi
Goto, Shunsaku
Ota, Shinji
Nagata, Yuichi
Nishihori, Masahiro
Maesawa, Satoshi
Izumi, Takashi
Takasu, Syuntaro
Saito, Ryuta
Higher abundance of Campylobacter in the oral microbiome of Japanese patients with moyamoya disease
title Higher abundance of Campylobacter in the oral microbiome of Japanese patients with moyamoya disease
title_full Higher abundance of Campylobacter in the oral microbiome of Japanese patients with moyamoya disease
title_fullStr Higher abundance of Campylobacter in the oral microbiome of Japanese patients with moyamoya disease
title_full_unstemmed Higher abundance of Campylobacter in the oral microbiome of Japanese patients with moyamoya disease
title_short Higher abundance of Campylobacter in the oral microbiome of Japanese patients with moyamoya disease
title_sort higher abundance of campylobacter in the oral microbiome of japanese patients with moyamoya disease
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10613609/
https://www.ncbi.nlm.nih.gov/pubmed/37899472
http://dx.doi.org/10.1038/s41598-023-45755-3
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