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Altered expression of circular RNA in patients with cervical artery dissection

Cervical artery dissection (CeAD), a special cerebrovascular disease and the main cause of stroke in young people, can present with ischemic stroke, headache, subarachnoid hemorrhage, and other symptoms, increasing the possibility of misdiagnosis. As a special class of non-coding RNAs, circRNAs are...

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Autores principales: Wang, Yifan, Dong, Zhaofei, Li, Jie, Li, Yudi, Mai, Jianyi, Tan, Wenru, Yang, Siqi, Ling, Li, Liu, Yajie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10613662/
https://www.ncbi.nlm.nih.gov/pubmed/37909033
http://dx.doi.org/10.3389/fneur.2023.1228400
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author Wang, Yifan
Dong, Zhaofei
Li, Jie
Li, Yudi
Mai, Jianyi
Tan, Wenru
Yang, Siqi
Ling, Li
Liu, Yajie
author_facet Wang, Yifan
Dong, Zhaofei
Li, Jie
Li, Yudi
Mai, Jianyi
Tan, Wenru
Yang, Siqi
Ling, Li
Liu, Yajie
author_sort Wang, Yifan
collection PubMed
description Cervical artery dissection (CeAD), a special cerebrovascular disease and the main cause of stroke in young people, can present with ischemic stroke, headache, subarachnoid hemorrhage, and other symptoms, increasing the possibility of misdiagnosis. As a special class of non-coding RNAs, circRNAs are commonly found in organisms and can play regulatory roles in transcription and post-transcription processes, affecting gene expression.CircRNAs have reported to be associated with neurological diseases; however, their role in CeAD has not been discerned. In this study, we aimed to elucidate the pathophysiological changes in patients with CeAD and identify biomarkers. Peripheral blood mononuclear cells from patients with CeAD and healthy controls were sequenced using high-throughput sequencing. We detected 460 differently expressed circRNAs in patients with CeAD (p < 0.5, fold difference ≥ 2), of which 240 were upregulated and 220 were downregulated. Four circRNAs showed significant differences in expression, which were validated using qRT-PCR. These results suggested that three circRNAs were consistent with high-throughput sequencing results. Bioinformatics analysis demonstrated that these differentially expressed circRNAs were involved in protein metabolism, regulation, synapses, and other pathophysiological processes during CeAD-induced stroke. Additionally, various pathways related to inflammation were closely associated with circRNAs. Based on our results, we suggest that the aberrant expression of circRNAs in CeAD may serve as a biomarker for its diagnosis and as a potential therapeutic target.
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spelling pubmed-106136622023-10-31 Altered expression of circular RNA in patients with cervical artery dissection Wang, Yifan Dong, Zhaofei Li, Jie Li, Yudi Mai, Jianyi Tan, Wenru Yang, Siqi Ling, Li Liu, Yajie Front Neurol Neurology Cervical artery dissection (CeAD), a special cerebrovascular disease and the main cause of stroke in young people, can present with ischemic stroke, headache, subarachnoid hemorrhage, and other symptoms, increasing the possibility of misdiagnosis. As a special class of non-coding RNAs, circRNAs are commonly found in organisms and can play regulatory roles in transcription and post-transcription processes, affecting gene expression.CircRNAs have reported to be associated with neurological diseases; however, their role in CeAD has not been discerned. In this study, we aimed to elucidate the pathophysiological changes in patients with CeAD and identify biomarkers. Peripheral blood mononuclear cells from patients with CeAD and healthy controls were sequenced using high-throughput sequencing. We detected 460 differently expressed circRNAs in patients with CeAD (p < 0.5, fold difference ≥ 2), of which 240 were upregulated and 220 were downregulated. Four circRNAs showed significant differences in expression, which were validated using qRT-PCR. These results suggested that three circRNAs were consistent with high-throughput sequencing results. Bioinformatics analysis demonstrated that these differentially expressed circRNAs were involved in protein metabolism, regulation, synapses, and other pathophysiological processes during CeAD-induced stroke. Additionally, various pathways related to inflammation were closely associated with circRNAs. Based on our results, we suggest that the aberrant expression of circRNAs in CeAD may serve as a biomarker for its diagnosis and as a potential therapeutic target. Frontiers Media S.A. 2023-10-16 /pmc/articles/PMC10613662/ /pubmed/37909033 http://dx.doi.org/10.3389/fneur.2023.1228400 Text en Copyright © 2023 Wang, Dong, Li, Li, Mai, Tan, Yang, Ling and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neurology
Wang, Yifan
Dong, Zhaofei
Li, Jie
Li, Yudi
Mai, Jianyi
Tan, Wenru
Yang, Siqi
Ling, Li
Liu, Yajie
Altered expression of circular RNA in patients with cervical artery dissection
title Altered expression of circular RNA in patients with cervical artery dissection
title_full Altered expression of circular RNA in patients with cervical artery dissection
title_fullStr Altered expression of circular RNA in patients with cervical artery dissection
title_full_unstemmed Altered expression of circular RNA in patients with cervical artery dissection
title_short Altered expression of circular RNA in patients with cervical artery dissection
title_sort altered expression of circular rna in patients with cervical artery dissection
topic Neurology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10613662/
https://www.ncbi.nlm.nih.gov/pubmed/37909033
http://dx.doi.org/10.3389/fneur.2023.1228400
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