Cargando…

Preliminary study of donor volume changes after dual-graft liver transplantation in rats

Dual-graft liver transplantation (DGLT) expands the pool of donors, ensures the safety of the donors, and treats a potential small for size syndrome (SFSS). However, some of the recipient graft showed atrophy. The cause and mechanism of the unbalanced proliferation and atrophy of dual grafts after c...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Dan, Ma, Yanling, Gu, Baohong, Li, Xuemei, Yu, Yang, Zhang, Ying, Chen, Hao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10613707/
https://www.ncbi.nlm.nih.gov/pubmed/37908343
http://dx.doi.org/10.3389/fimmu.2023.1183426
_version_ 1785128888962121728
author Wang, Dan
Ma, Yanling
Gu, Baohong
Li, Xuemei
Yu, Yang
Zhang, Ying
Chen, Hao
author_facet Wang, Dan
Ma, Yanling
Gu, Baohong
Li, Xuemei
Yu, Yang
Zhang, Ying
Chen, Hao
author_sort Wang, Dan
collection PubMed
description Dual-graft liver transplantation (DGLT) expands the pool of donors, ensures the safety of the donors, and treats a potential small for size syndrome (SFSS). However, some of the recipient graft showed atrophy. The cause and mechanism of the unbalanced proliferation and atrophy of dual grafts after clinical DGLT have not been clarified. We established and optimized the rat model of DGLT to explore the causes of growth unbalance. Continuously and dynamically observed bilateral graft volume and portal vein blood flow change by magnetic resonance imaging (MRI) and ultrasound (US). We detected liver function indexes: alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), total bilirubin (TBIL), direct bilirubin (DBIL), and indirect bilirubin (IBIL). Liver samples from receptors were obtained for morphology, and apoptosis was measured by RT-PCR and western blot. Optimization of the model improved the 7-day survival rate from former 58.3% to 87.5%, and the 30-day survival rate was 68.8%. The volume of the right graft gradually increased, and the left graft atrophied during the 30-day observation period. The portal blood flow of the left graft gradually decreased until the 30th day (0.13 ± 0.01 ml/s) compared with the sham group (0.63 ± 0.05 ml/s), and the right graft significantly increased on the 30th day (0.75 ± 0.11ml/s). The liver function initially increased and then recovered. The total volume (12.52 ± 1.60 ml vs 4.47 ± 0.08 ml) and weight (12.09 ± 1 g vs 4.91 ± 0.18 g) of the graft increased significantly compared to pre-transplantation and reached the level of the sham operation group on the 30th day. The volume and weight of the right graft increased more than those of the left graft (P < 0.05). There was more inflammatory cell infiltration in the left graft, and the right graft had obvious proliferation of hepatocytes and mature bile duct cells. Left grafts were more prone to apoptosis than right grafts (P < 0.05). In conclusion, growth of the right graft is superior to the left; after double liver transplantation, perfusion blood flow and apoptosis may be the reason contributing to the volume differences in dual grafts.
format Online
Article
Text
id pubmed-10613707
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-106137072023-10-31 Preliminary study of donor volume changes after dual-graft liver transplantation in rats Wang, Dan Ma, Yanling Gu, Baohong Li, Xuemei Yu, Yang Zhang, Ying Chen, Hao Front Immunol Immunology Dual-graft liver transplantation (DGLT) expands the pool of donors, ensures the safety of the donors, and treats a potential small for size syndrome (SFSS). However, some of the recipient graft showed atrophy. The cause and mechanism of the unbalanced proliferation and atrophy of dual grafts after clinical DGLT have not been clarified. We established and optimized the rat model of DGLT to explore the causes of growth unbalance. Continuously and dynamically observed bilateral graft volume and portal vein blood flow change by magnetic resonance imaging (MRI) and ultrasound (US). We detected liver function indexes: alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), total bilirubin (TBIL), direct bilirubin (DBIL), and indirect bilirubin (IBIL). Liver samples from receptors were obtained for morphology, and apoptosis was measured by RT-PCR and western blot. Optimization of the model improved the 7-day survival rate from former 58.3% to 87.5%, and the 30-day survival rate was 68.8%. The volume of the right graft gradually increased, and the left graft atrophied during the 30-day observation period. The portal blood flow of the left graft gradually decreased until the 30th day (0.13 ± 0.01 ml/s) compared with the sham group (0.63 ± 0.05 ml/s), and the right graft significantly increased on the 30th day (0.75 ± 0.11ml/s). The liver function initially increased and then recovered. The total volume (12.52 ± 1.60 ml vs 4.47 ± 0.08 ml) and weight (12.09 ± 1 g vs 4.91 ± 0.18 g) of the graft increased significantly compared to pre-transplantation and reached the level of the sham operation group on the 30th day. The volume and weight of the right graft increased more than those of the left graft (P < 0.05). There was more inflammatory cell infiltration in the left graft, and the right graft had obvious proliferation of hepatocytes and mature bile duct cells. Left grafts were more prone to apoptosis than right grafts (P < 0.05). In conclusion, growth of the right graft is superior to the left; after double liver transplantation, perfusion blood flow and apoptosis may be the reason contributing to the volume differences in dual grafts. Frontiers Media S.A. 2023-10-16 /pmc/articles/PMC10613707/ /pubmed/37908343 http://dx.doi.org/10.3389/fimmu.2023.1183426 Text en Copyright © 2023 Wang, Ma, Gu, Li, Yu, Zhang and Chen https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Wang, Dan
Ma, Yanling
Gu, Baohong
Li, Xuemei
Yu, Yang
Zhang, Ying
Chen, Hao
Preliminary study of donor volume changes after dual-graft liver transplantation in rats
title Preliminary study of donor volume changes after dual-graft liver transplantation in rats
title_full Preliminary study of donor volume changes after dual-graft liver transplantation in rats
title_fullStr Preliminary study of donor volume changes after dual-graft liver transplantation in rats
title_full_unstemmed Preliminary study of donor volume changes after dual-graft liver transplantation in rats
title_short Preliminary study of donor volume changes after dual-graft liver transplantation in rats
title_sort preliminary study of donor volume changes after dual-graft liver transplantation in rats
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10613707/
https://www.ncbi.nlm.nih.gov/pubmed/37908343
http://dx.doi.org/10.3389/fimmu.2023.1183426
work_keys_str_mv AT wangdan preliminarystudyofdonorvolumechangesafterdualgraftlivertransplantationinrats
AT mayanling preliminarystudyofdonorvolumechangesafterdualgraftlivertransplantationinrats
AT gubaohong preliminarystudyofdonorvolumechangesafterdualgraftlivertransplantationinrats
AT lixuemei preliminarystudyofdonorvolumechangesafterdualgraftlivertransplantationinrats
AT yuyang preliminarystudyofdonorvolumechangesafterdualgraftlivertransplantationinrats
AT zhangying preliminarystudyofdonorvolumechangesafterdualgraftlivertransplantationinrats
AT chenhao preliminarystudyofdonorvolumechangesafterdualgraftlivertransplantationinrats