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Faecal metabolome responses to an altered dietary protein:carbohydrate ratio in adult dogs
High-protein diets may aid weight loss and weight maintenance programs in both humans and dogs, although the effect of dietary protein levels on gut metabolism and functionality has not been studied in depth. The current study aimed to investigate the effect of an altered dietary protein:carbohydrat...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10614716/ https://www.ncbi.nlm.nih.gov/pubmed/37869782 http://dx.doi.org/10.1080/01652176.2023.2273891 |
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author | Lyu, Yang Xu, Jia Verdoodt, Fien Vanhaecke, Lynn Hemeryck, Lieselot Y. Hesta, Myriam |
author_facet | Lyu, Yang Xu, Jia Verdoodt, Fien Vanhaecke, Lynn Hemeryck, Lieselot Y. Hesta, Myriam |
author_sort | Lyu, Yang |
collection | PubMed |
description | High-protein diets may aid weight loss and weight maintenance programs in both humans and dogs, although the effect of dietary protein levels on gut metabolism and functionality has not been studied in depth. The current study aimed to investigate the effect of an altered dietary protein:carbohydrate ratio on gut function in adult dogs by means of faecal metabolomic fingerprinting. More specifically, functional metabolic differences in dogs fed a high-protein/low-carbohydrate (HPLC) vs. low-protein/high-carbohydrate (LPHC) diet were studied by equally allocating twelve clinically healthy (6 lean and 6 obese) Beagles into two groups in a cross-over design, with each group receiving two isocaloric diets for four weeks. The faecal metabolome revealed that different protein:carbohydrate ratio can influence host and/or gut microbiome metabolism and function, while no effect was observed on the body condition. Targeted analysis demonstrated that the HPLC diet significantly increased the concentration of indole, spermidine, and pipecolinic acid and decreased the concentration of azelaic acid, D-fructose, mannose, and galactose (p < 0.05). Multivariate modelling (OPLS-DA) of the untargeted faecal metabolome revealed distinctly different metabolomic profiles following the HPLC vs. LPHC diet, with 18 altered pathways. The HPLC diet influenced amino acid and lipid metabolism, potentially promoting weight loss and immune function, whereas the LPHC diet affected carbohydrate fermentation and may promote anti-oxidative function. |
format | Online Article Text |
id | pubmed-10614716 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-106147162023-10-31 Faecal metabolome responses to an altered dietary protein:carbohydrate ratio in adult dogs Lyu, Yang Xu, Jia Verdoodt, Fien Vanhaecke, Lynn Hemeryck, Lieselot Y. Hesta, Myriam Vet Q Research Article High-protein diets may aid weight loss and weight maintenance programs in both humans and dogs, although the effect of dietary protein levels on gut metabolism and functionality has not been studied in depth. The current study aimed to investigate the effect of an altered dietary protein:carbohydrate ratio on gut function in adult dogs by means of faecal metabolomic fingerprinting. More specifically, functional metabolic differences in dogs fed a high-protein/low-carbohydrate (HPLC) vs. low-protein/high-carbohydrate (LPHC) diet were studied by equally allocating twelve clinically healthy (6 lean and 6 obese) Beagles into two groups in a cross-over design, with each group receiving two isocaloric diets for four weeks. The faecal metabolome revealed that different protein:carbohydrate ratio can influence host and/or gut microbiome metabolism and function, while no effect was observed on the body condition. Targeted analysis demonstrated that the HPLC diet significantly increased the concentration of indole, spermidine, and pipecolinic acid and decreased the concentration of azelaic acid, D-fructose, mannose, and galactose (p < 0.05). Multivariate modelling (OPLS-DA) of the untargeted faecal metabolome revealed distinctly different metabolomic profiles following the HPLC vs. LPHC diet, with 18 altered pathways. The HPLC diet influenced amino acid and lipid metabolism, potentially promoting weight loss and immune function, whereas the LPHC diet affected carbohydrate fermentation and may promote anti-oxidative function. Taylor & Francis 2023-10-28 /pmc/articles/PMC10614716/ /pubmed/37869782 http://dx.doi.org/10.1080/01652176.2023.2273891 Text en © 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent. |
spellingShingle | Research Article Lyu, Yang Xu, Jia Verdoodt, Fien Vanhaecke, Lynn Hemeryck, Lieselot Y. Hesta, Myriam Faecal metabolome responses to an altered dietary protein:carbohydrate ratio in adult dogs |
title | Faecal metabolome responses to an altered dietary protein:carbohydrate ratio in adult dogs |
title_full | Faecal metabolome responses to an altered dietary protein:carbohydrate ratio in adult dogs |
title_fullStr | Faecal metabolome responses to an altered dietary protein:carbohydrate ratio in adult dogs |
title_full_unstemmed | Faecal metabolome responses to an altered dietary protein:carbohydrate ratio in adult dogs |
title_short | Faecal metabolome responses to an altered dietary protein:carbohydrate ratio in adult dogs |
title_sort | faecal metabolome responses to an altered dietary protein:carbohydrate ratio in adult dogs |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10614716/ https://www.ncbi.nlm.nih.gov/pubmed/37869782 http://dx.doi.org/10.1080/01652176.2023.2273891 |
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