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Sibling chimerism among microglia in marmosets

Chimerism happens rarely among most mammals but is common in marmosets and tamarins, a result of fraternal twin or triplet birth patterns in which in utero connected circulatory systems (through which stem cells transit) lead to persistent blood chimerism (12–80%) throughout life. The presence of Y-...

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Autores principales: del Rosario, Ricardo C.H., Krienen, Fenna M., Zhang, Qiangge, Goldman, Melissa, Mello, Curtis, Lutservitz, Alyssa, Ichihara, Kiku, Wysoker, Alec, Nemesh, James, Feng, Guoping, McCarroll, Steven A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10614798/
https://www.ncbi.nlm.nih.gov/pubmed/37904944
http://dx.doi.org/10.1101/2023.10.16.562516
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author del Rosario, Ricardo C.H.
Krienen, Fenna M.
Zhang, Qiangge
Goldman, Melissa
Mello, Curtis
Lutservitz, Alyssa
Ichihara, Kiku
Wysoker, Alec
Nemesh, James
Feng, Guoping
McCarroll, Steven A.
author_facet del Rosario, Ricardo C.H.
Krienen, Fenna M.
Zhang, Qiangge
Goldman, Melissa
Mello, Curtis
Lutservitz, Alyssa
Ichihara, Kiku
Wysoker, Alec
Nemesh, James
Feng, Guoping
McCarroll, Steven A.
author_sort del Rosario, Ricardo C.H.
collection PubMed
description Chimerism happens rarely among most mammals but is common in marmosets and tamarins, a result of fraternal twin or triplet birth patterns in which in utero connected circulatory systems (through which stem cells transit) lead to persistent blood chimerism (12–80%) throughout life. The presence of Y-chromosome DNA sequences in other organs of female marmosets has long suggested that chimerism might also affect these organs. However, a longstanding question is whether this chimerism is driven by blood-derived cells or involves contributions from other cell types. To address this question, we analyzed single-cell RNA-seq data from blood, liver, kidney and multiple brain regions across a number of marmosets, using transcribed single nucleotide polymorphisms (SNPs) to identify cells with the sibling’s genome in various cell types within these tissues. Sibling-derived chimerism in all tissues arose entirely from cells of hematopoietic origin (i.e., myeloid and lymphoid lineages). In brain tissue this was reflected as sibling-derived chimerism among microglia (20–52%) and macrophages (18–64%) but not among other resident cell types (i.e., neurons, glia or ependymal cells). The percentage of microglia that were sibling-derived showed significant variation across brain regions, even within individual animals, likely reflecting distinct responses by siblings’ microglia to local recruitment or proliferation cues or, potentially, distinct clonal expansion histories in different brain areas. In the animals and tissues we analyzed, microglial gene expression profiles bore a much stronger relationship to local/host context than to sibling genetic differences. Naturally occurring marmoset chimerism will provide new ways to understand the effects of genes, mutations and brain contexts on microglial biology and to distinguish between effects of microglia and other cell types on brain phenotypes.
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spelling pubmed-106147982023-10-31 Sibling chimerism among microglia in marmosets del Rosario, Ricardo C.H. Krienen, Fenna M. Zhang, Qiangge Goldman, Melissa Mello, Curtis Lutservitz, Alyssa Ichihara, Kiku Wysoker, Alec Nemesh, James Feng, Guoping McCarroll, Steven A. bioRxiv Article Chimerism happens rarely among most mammals but is common in marmosets and tamarins, a result of fraternal twin or triplet birth patterns in which in utero connected circulatory systems (through which stem cells transit) lead to persistent blood chimerism (12–80%) throughout life. The presence of Y-chromosome DNA sequences in other organs of female marmosets has long suggested that chimerism might also affect these organs. However, a longstanding question is whether this chimerism is driven by blood-derived cells or involves contributions from other cell types. To address this question, we analyzed single-cell RNA-seq data from blood, liver, kidney and multiple brain regions across a number of marmosets, using transcribed single nucleotide polymorphisms (SNPs) to identify cells with the sibling’s genome in various cell types within these tissues. Sibling-derived chimerism in all tissues arose entirely from cells of hematopoietic origin (i.e., myeloid and lymphoid lineages). In brain tissue this was reflected as sibling-derived chimerism among microglia (20–52%) and macrophages (18–64%) but not among other resident cell types (i.e., neurons, glia or ependymal cells). The percentage of microglia that were sibling-derived showed significant variation across brain regions, even within individual animals, likely reflecting distinct responses by siblings’ microglia to local recruitment or proliferation cues or, potentially, distinct clonal expansion histories in different brain areas. In the animals and tissues we analyzed, microglial gene expression profiles bore a much stronger relationship to local/host context than to sibling genetic differences. Naturally occurring marmoset chimerism will provide new ways to understand the effects of genes, mutations and brain contexts on microglial biology and to distinguish between effects of microglia and other cell types on brain phenotypes. Cold Spring Harbor Laboratory 2023-10-17 /pmc/articles/PMC10614798/ /pubmed/37904944 http://dx.doi.org/10.1101/2023.10.16.562516 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which allows reusers to copy and distribute the material in any medium or format in unadapted form only, for noncommercial purposes only, and only so long as attribution is given to the creator.
spellingShingle Article
del Rosario, Ricardo C.H.
Krienen, Fenna M.
Zhang, Qiangge
Goldman, Melissa
Mello, Curtis
Lutservitz, Alyssa
Ichihara, Kiku
Wysoker, Alec
Nemesh, James
Feng, Guoping
McCarroll, Steven A.
Sibling chimerism among microglia in marmosets
title Sibling chimerism among microglia in marmosets
title_full Sibling chimerism among microglia in marmosets
title_fullStr Sibling chimerism among microglia in marmosets
title_full_unstemmed Sibling chimerism among microglia in marmosets
title_short Sibling chimerism among microglia in marmosets
title_sort sibling chimerism among microglia in marmosets
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10614798/
https://www.ncbi.nlm.nih.gov/pubmed/37904944
http://dx.doi.org/10.1101/2023.10.16.562516
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