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CRISPR interference screens reveal growth–robustness tradeoffs in Synechocystis sp. PCC 6803 across growth conditions

Barcoded mutant libraries are a powerful tool for elucidating gene function in microbes, particularly when screened in multiple growth conditions. Here, we screened a pooled CRISPR interference library of the model cyanobacterium Synechocystis sp. PCC 6803 in 11 bioreactor-controlled conditions, spa...

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Detalles Bibliográficos
Autores principales:	Miao, Rui, Jahn, Michael, Shabestary, Kiyan, Peltier, Gilles, Hudson, Elton P
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2023
Materias:	Large-scale Biology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10615215/ https://www.ncbi.nlm.nih.gov/pubmed/37494719 http://dx.doi.org/10.1093/plcell/koad208

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