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Isolation, draft genome sequence, and identification of Paenibacillus glycanilyticus subsp. hiroshimensis CCS26
To isolate the useful strain for fermentation to produce bioactive compounds, we screened oligotrophic bacteria, and then strain CCS26 was isolated from leaf soil collected in Japan. This strain was capable of growth on low-nutrient medium. To elucidate the taxonomy of strain CCS26, we determined th...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Ivyspring International Publisher
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10615617/ https://www.ncbi.nlm.nih.gov/pubmed/37915958 http://dx.doi.org/10.7150/jgen.87229 |
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author | Akita, Hironaga Shinto, Yoshiki Kimura, Zen-ichiro |
author_facet | Akita, Hironaga Shinto, Yoshiki Kimura, Zen-ichiro |
author_sort | Akita, Hironaga |
collection | PubMed |
description | To isolate the useful strain for fermentation to produce bioactive compounds, we screened oligotrophic bacteria, and then strain CCS26 was isolated from leaf soil collected in Japan. This strain was capable of growth on low-nutrient medium. To elucidate the taxonomy of strain CCS26, we determined the 16S rRNA gene and draft genome sequences, respectively. A phylogenetic tree based on 16S rRNA gene sequences showed that strain CCS26 clustered with Paenibacillus species. The draft genome sequence of strain CCS26 consisted of a total of 90 contigs containing 6,957,994 bp, with a GC content of 50.8% and comprising 6,343 predicted coding sequences. Based on analysis of the average nucleotide identity with the draft genome sequence, the strain was identified as P. glycanilyticus subsp. hiroshimensis CCS26. |
format | Online Article Text |
id | pubmed-10615617 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-106156172023-11-01 Isolation, draft genome sequence, and identification of Paenibacillus glycanilyticus subsp. hiroshimensis CCS26 Akita, Hironaga Shinto, Yoshiki Kimura, Zen-ichiro J Genomics Research Paper To isolate the useful strain for fermentation to produce bioactive compounds, we screened oligotrophic bacteria, and then strain CCS26 was isolated from leaf soil collected in Japan. This strain was capable of growth on low-nutrient medium. To elucidate the taxonomy of strain CCS26, we determined the 16S rRNA gene and draft genome sequences, respectively. A phylogenetic tree based on 16S rRNA gene sequences showed that strain CCS26 clustered with Paenibacillus species. The draft genome sequence of strain CCS26 consisted of a total of 90 contigs containing 6,957,994 bp, with a GC content of 50.8% and comprising 6,343 predicted coding sequences. Based on analysis of the average nucleotide identity with the draft genome sequence, the strain was identified as P. glycanilyticus subsp. hiroshimensis CCS26. Ivyspring International Publisher 2023-09-25 /pmc/articles/PMC10615617/ /pubmed/37915958 http://dx.doi.org/10.7150/jgen.87229 Text en © The author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions. |
spellingShingle | Research Paper Akita, Hironaga Shinto, Yoshiki Kimura, Zen-ichiro Isolation, draft genome sequence, and identification of Paenibacillus glycanilyticus subsp. hiroshimensis CCS26 |
title | Isolation, draft genome sequence, and identification of Paenibacillus glycanilyticus subsp. hiroshimensis CCS26 |
title_full | Isolation, draft genome sequence, and identification of Paenibacillus glycanilyticus subsp. hiroshimensis CCS26 |
title_fullStr | Isolation, draft genome sequence, and identification of Paenibacillus glycanilyticus subsp. hiroshimensis CCS26 |
title_full_unstemmed | Isolation, draft genome sequence, and identification of Paenibacillus glycanilyticus subsp. hiroshimensis CCS26 |
title_short | Isolation, draft genome sequence, and identification of Paenibacillus glycanilyticus subsp. hiroshimensis CCS26 |
title_sort | isolation, draft genome sequence, and identification of paenibacillus glycanilyticus subsp. hiroshimensis ccs26 |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10615617/ https://www.ncbi.nlm.nih.gov/pubmed/37915958 http://dx.doi.org/10.7150/jgen.87229 |
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