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A novel zinc finger transcription factor, BcMsn2, is involved in growth, development, and virulence in Botrytis cinerea

Reactive oxygen species (ROS) are important for plant defense against fungal attack. As a necrotrophic fungus, Botrytis cinerea can exploit ROS that originated from both sides of the host and pathogen during interaction to facilitate its infestation. Meanwhile, B. cinerea needs to exert an efficient...

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Autores principales: Lu, Ping, Wang, Ke, Wang, Jiaqi, Xia, Chunbo, Yang, Shu, Ma, Liang, Shi, Haojie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10616473/
https://www.ncbi.nlm.nih.gov/pubmed/37915851
http://dx.doi.org/10.3389/fmicb.2023.1247072
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author Lu, Ping
Wang, Ke
Wang, Jiaqi
Xia, Chunbo
Yang, Shu
Ma, Liang
Shi, Haojie
author_facet Lu, Ping
Wang, Ke
Wang, Jiaqi
Xia, Chunbo
Yang, Shu
Ma, Liang
Shi, Haojie
author_sort Lu, Ping
collection PubMed
description Reactive oxygen species (ROS) are important for plant defense against fungal attack. As a necrotrophic fungus, Botrytis cinerea can exploit ROS that originated from both sides of the host and pathogen during interaction to facilitate its infestation. Meanwhile, B. cinerea needs to exert an efficient oxidative stress responsive system to balance the intracellular redox state when encountering deleterious ROS levels. However, the machinery applied by B. cinerea to cope with ROS remains obscure. Herein, we investigated the role of the transcription factor BcMsn2 in regulating B. cinerea redox homeostasis. Disruption of the BcMsn2 gene severely impaired vegetative growth, sclerotium formation, conidial yield, and fungal virulence. The intracellular oxidative homeostasis of the ∆bcmsn2 mutant was disrupted, leading to significantly elevated levels of ROS and reduced activities of enzymes closely associated with oxygen stress, such as catalase (CAT) and superoxide dismutase (SOD). RNA-Seq and qRT-PCR analyses showed remarkable downregulation of the expression of several genes encoding ROS scavenging factors involved in maintaining the redox homeostasis in ∆bcmsn2, suggesting that BcMsn2 functions as a transcriptional regulator of these genes. Our findings indicated that BcMsn2 plays an indispensable role in maintaining the equilibrium of the redox state in B. cinerea, and intracellular ROS serve as signaling molecules that regulate the growth, asexual reproduction, and virulence of this pathogen.
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spelling pubmed-106164732023-11-01 A novel zinc finger transcription factor, BcMsn2, is involved in growth, development, and virulence in Botrytis cinerea Lu, Ping Wang, Ke Wang, Jiaqi Xia, Chunbo Yang, Shu Ma, Liang Shi, Haojie Front Microbiol Microbiology Reactive oxygen species (ROS) are important for plant defense against fungal attack. As a necrotrophic fungus, Botrytis cinerea can exploit ROS that originated from both sides of the host and pathogen during interaction to facilitate its infestation. Meanwhile, B. cinerea needs to exert an efficient oxidative stress responsive system to balance the intracellular redox state when encountering deleterious ROS levels. However, the machinery applied by B. cinerea to cope with ROS remains obscure. Herein, we investigated the role of the transcription factor BcMsn2 in regulating B. cinerea redox homeostasis. Disruption of the BcMsn2 gene severely impaired vegetative growth, sclerotium formation, conidial yield, and fungal virulence. The intracellular oxidative homeostasis of the ∆bcmsn2 mutant was disrupted, leading to significantly elevated levels of ROS and reduced activities of enzymes closely associated with oxygen stress, such as catalase (CAT) and superoxide dismutase (SOD). RNA-Seq and qRT-PCR analyses showed remarkable downregulation of the expression of several genes encoding ROS scavenging factors involved in maintaining the redox homeostasis in ∆bcmsn2, suggesting that BcMsn2 functions as a transcriptional regulator of these genes. Our findings indicated that BcMsn2 plays an indispensable role in maintaining the equilibrium of the redox state in B. cinerea, and intracellular ROS serve as signaling molecules that regulate the growth, asexual reproduction, and virulence of this pathogen. Frontiers Media S.A. 2023-10-17 /pmc/articles/PMC10616473/ /pubmed/37915851 http://dx.doi.org/10.3389/fmicb.2023.1247072 Text en Copyright © 2023 Lu, Wang, Wang, Xia, Yang, Ma and Shi. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Lu, Ping
Wang, Ke
Wang, Jiaqi
Xia, Chunbo
Yang, Shu
Ma, Liang
Shi, Haojie
A novel zinc finger transcription factor, BcMsn2, is involved in growth, development, and virulence in Botrytis cinerea
title A novel zinc finger transcription factor, BcMsn2, is involved in growth, development, and virulence in Botrytis cinerea
title_full A novel zinc finger transcription factor, BcMsn2, is involved in growth, development, and virulence in Botrytis cinerea
title_fullStr A novel zinc finger transcription factor, BcMsn2, is involved in growth, development, and virulence in Botrytis cinerea
title_full_unstemmed A novel zinc finger transcription factor, BcMsn2, is involved in growth, development, and virulence in Botrytis cinerea
title_short A novel zinc finger transcription factor, BcMsn2, is involved in growth, development, and virulence in Botrytis cinerea
title_sort novel zinc finger transcription factor, bcmsn2, is involved in growth, development, and virulence in botrytis cinerea
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10616473/
https://www.ncbi.nlm.nih.gov/pubmed/37915851
http://dx.doi.org/10.3389/fmicb.2023.1247072
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