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Holistic view of heat acclimation alleviated intestinal lesion in mice with heat stroke based on microbiome‐metabolomics analysis

The severity of heat stroke (HS) is associated with intestinal injury, which is generally considered an essential issue for HS. Heat acclimation (HA) is considered the best strategy to protect against HS. In addition, HA has a protective effect on intestinal injuries caused by HS. Considering the es...

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Autores principales: Huang, Xueyan, Luo, Zhen, Shen, Tingting, He, Genlin, Yu, Xueting, Liu, Yishan, Tan, Yulong, Wang, Zeze, Li, Ping, Zhang, Boyi, Zhou, Huan, Chen, Xiangyu, Wang, Ying, Yu, Shasha, Luo, Xue, Yang, Xuesen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10616642/
https://www.ncbi.nlm.nih.gov/pubmed/37792264
http://dx.doi.org/10.1111/1751-7915.14349
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author Huang, Xueyan
Luo, Zhen
Shen, Tingting
He, Genlin
Yu, Xueting
Liu, Yishan
Tan, Yulong
Wang, Zeze
Li, Ping
Zhang, Boyi
Zhou, Huan
Chen, Xiangyu
Wang, Ying
Yu, Shasha
Luo, Xue
Yang, Xuesen
author_facet Huang, Xueyan
Luo, Zhen
Shen, Tingting
He, Genlin
Yu, Xueting
Liu, Yishan
Tan, Yulong
Wang, Zeze
Li, Ping
Zhang, Boyi
Zhou, Huan
Chen, Xiangyu
Wang, Ying
Yu, Shasha
Luo, Xue
Yang, Xuesen
author_sort Huang, Xueyan
collection PubMed
description The severity of heat stroke (HS) is associated with intestinal injury, which is generally considered an essential issue for HS. Heat acclimation (HA) is considered the best strategy to protect against HS. In addition, HA has a protective effect on intestinal injuries caused by HS. Considering the essential role of gut microbes in intestinal structure and function, we decided to investigate the potential protective mechanism of HA in reducing intestinal injury caused by HS. HA model was established by male C57BL/6J mice (5–6 weeks old, 17–19 g) were exposed at (34 ± 0.7)°C for 4 weeks to establish an animal HA model. The protective effect of HA on intestinal barrier injury in HS was investigated by 16S rRNA gene sequencing and nontargeted liquid chromatography‐mass spectrometry (LC‐MS) metabolomics. According to the experimental results, HA can change the composition of the gut microbiota, which increases the proportion of lactobacilli, faecal bacteria, and urinobacteria but decreases the proportion of deoxycholic acid. Moreover, HA can reduce liver and kidney injury and systemic inflammation caused by HS and reduce intestinal injury by enhancing the integrity of the intestinal barrier. In addition, HA regulates inflammation by inhibiting NF‐κB signalling and increasing tight junction protein expression in HS mice. HA induces changes in the gut microbiota, which may enhance tight junction protein expression, thereby reducing intestinal inflammation, promoting bile acid metabolism, and ultimately maintaining the integrity of the intestinal barrier. In conclusion, HA induced changes in the gut microbiota. Among the gut microbiota, lactobacilli may play a key role in the potential protective mechanism of HA.
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spelling pubmed-106166422023-11-01 Holistic view of heat acclimation alleviated intestinal lesion in mice with heat stroke based on microbiome‐metabolomics analysis Huang, Xueyan Luo, Zhen Shen, Tingting He, Genlin Yu, Xueting Liu, Yishan Tan, Yulong Wang, Zeze Li, Ping Zhang, Boyi Zhou, Huan Chen, Xiangyu Wang, Ying Yu, Shasha Luo, Xue Yang, Xuesen Microb Biotechnol Research Articles The severity of heat stroke (HS) is associated with intestinal injury, which is generally considered an essential issue for HS. Heat acclimation (HA) is considered the best strategy to protect against HS. In addition, HA has a protective effect on intestinal injuries caused by HS. Considering the essential role of gut microbes in intestinal structure and function, we decided to investigate the potential protective mechanism of HA in reducing intestinal injury caused by HS. HA model was established by male C57BL/6J mice (5–6 weeks old, 17–19 g) were exposed at (34 ± 0.7)°C for 4 weeks to establish an animal HA model. The protective effect of HA on intestinal barrier injury in HS was investigated by 16S rRNA gene sequencing and nontargeted liquid chromatography‐mass spectrometry (LC‐MS) metabolomics. According to the experimental results, HA can change the composition of the gut microbiota, which increases the proportion of lactobacilli, faecal bacteria, and urinobacteria but decreases the proportion of deoxycholic acid. Moreover, HA can reduce liver and kidney injury and systemic inflammation caused by HS and reduce intestinal injury by enhancing the integrity of the intestinal barrier. In addition, HA regulates inflammation by inhibiting NF‐κB signalling and increasing tight junction protein expression in HS mice. HA induces changes in the gut microbiota, which may enhance tight junction protein expression, thereby reducing intestinal inflammation, promoting bile acid metabolism, and ultimately maintaining the integrity of the intestinal barrier. In conclusion, HA induced changes in the gut microbiota. Among the gut microbiota, lactobacilli may play a key role in the potential protective mechanism of HA. John Wiley and Sons Inc. 2023-10-04 /pmc/articles/PMC10616642/ /pubmed/37792264 http://dx.doi.org/10.1111/1751-7915.14349 Text en © 2023 The Authors. Microbial Biotechnology published by Applied Microbiology International and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Research Articles
Huang, Xueyan
Luo, Zhen
Shen, Tingting
He, Genlin
Yu, Xueting
Liu, Yishan
Tan, Yulong
Wang, Zeze
Li, Ping
Zhang, Boyi
Zhou, Huan
Chen, Xiangyu
Wang, Ying
Yu, Shasha
Luo, Xue
Yang, Xuesen
Holistic view of heat acclimation alleviated intestinal lesion in mice with heat stroke based on microbiome‐metabolomics analysis
title Holistic view of heat acclimation alleviated intestinal lesion in mice with heat stroke based on microbiome‐metabolomics analysis
title_full Holistic view of heat acclimation alleviated intestinal lesion in mice with heat stroke based on microbiome‐metabolomics analysis
title_fullStr Holistic view of heat acclimation alleviated intestinal lesion in mice with heat stroke based on microbiome‐metabolomics analysis
title_full_unstemmed Holistic view of heat acclimation alleviated intestinal lesion in mice with heat stroke based on microbiome‐metabolomics analysis
title_short Holistic view of heat acclimation alleviated intestinal lesion in mice with heat stroke based on microbiome‐metabolomics analysis
title_sort holistic view of heat acclimation alleviated intestinal lesion in mice with heat stroke based on microbiome‐metabolomics analysis
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10616642/
https://www.ncbi.nlm.nih.gov/pubmed/37792264
http://dx.doi.org/10.1111/1751-7915.14349
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