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Holistic view of heat acclimation alleviated intestinal lesion in mice with heat stroke based on microbiome‐metabolomics analysis
The severity of heat stroke (HS) is associated with intestinal injury, which is generally considered an essential issue for HS. Heat acclimation (HA) is considered the best strategy to protect against HS. In addition, HA has a protective effect on intestinal injuries caused by HS. Considering the es...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10616642/ https://www.ncbi.nlm.nih.gov/pubmed/37792264 http://dx.doi.org/10.1111/1751-7915.14349 |
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author | Huang, Xueyan Luo, Zhen Shen, Tingting He, Genlin Yu, Xueting Liu, Yishan Tan, Yulong Wang, Zeze Li, Ping Zhang, Boyi Zhou, Huan Chen, Xiangyu Wang, Ying Yu, Shasha Luo, Xue Yang, Xuesen |
author_facet | Huang, Xueyan Luo, Zhen Shen, Tingting He, Genlin Yu, Xueting Liu, Yishan Tan, Yulong Wang, Zeze Li, Ping Zhang, Boyi Zhou, Huan Chen, Xiangyu Wang, Ying Yu, Shasha Luo, Xue Yang, Xuesen |
author_sort | Huang, Xueyan |
collection | PubMed |
description | The severity of heat stroke (HS) is associated with intestinal injury, which is generally considered an essential issue for HS. Heat acclimation (HA) is considered the best strategy to protect against HS. In addition, HA has a protective effect on intestinal injuries caused by HS. Considering the essential role of gut microbes in intestinal structure and function, we decided to investigate the potential protective mechanism of HA in reducing intestinal injury caused by HS. HA model was established by male C57BL/6J mice (5–6 weeks old, 17–19 g) were exposed at (34 ± 0.7)°C for 4 weeks to establish an animal HA model. The protective effect of HA on intestinal barrier injury in HS was investigated by 16S rRNA gene sequencing and nontargeted liquid chromatography‐mass spectrometry (LC‐MS) metabolomics. According to the experimental results, HA can change the composition of the gut microbiota, which increases the proportion of lactobacilli, faecal bacteria, and urinobacteria but decreases the proportion of deoxycholic acid. Moreover, HA can reduce liver and kidney injury and systemic inflammation caused by HS and reduce intestinal injury by enhancing the integrity of the intestinal barrier. In addition, HA regulates inflammation by inhibiting NF‐κB signalling and increasing tight junction protein expression in HS mice. HA induces changes in the gut microbiota, which may enhance tight junction protein expression, thereby reducing intestinal inflammation, promoting bile acid metabolism, and ultimately maintaining the integrity of the intestinal barrier. In conclusion, HA induced changes in the gut microbiota. Among the gut microbiota, lactobacilli may play a key role in the potential protective mechanism of HA. |
format | Online Article Text |
id | pubmed-10616642 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-106166422023-11-01 Holistic view of heat acclimation alleviated intestinal lesion in mice with heat stroke based on microbiome‐metabolomics analysis Huang, Xueyan Luo, Zhen Shen, Tingting He, Genlin Yu, Xueting Liu, Yishan Tan, Yulong Wang, Zeze Li, Ping Zhang, Boyi Zhou, Huan Chen, Xiangyu Wang, Ying Yu, Shasha Luo, Xue Yang, Xuesen Microb Biotechnol Research Articles The severity of heat stroke (HS) is associated with intestinal injury, which is generally considered an essential issue for HS. Heat acclimation (HA) is considered the best strategy to protect against HS. In addition, HA has a protective effect on intestinal injuries caused by HS. Considering the essential role of gut microbes in intestinal structure and function, we decided to investigate the potential protective mechanism of HA in reducing intestinal injury caused by HS. HA model was established by male C57BL/6J mice (5–6 weeks old, 17–19 g) were exposed at (34 ± 0.7)°C for 4 weeks to establish an animal HA model. The protective effect of HA on intestinal barrier injury in HS was investigated by 16S rRNA gene sequencing and nontargeted liquid chromatography‐mass spectrometry (LC‐MS) metabolomics. According to the experimental results, HA can change the composition of the gut microbiota, which increases the proportion of lactobacilli, faecal bacteria, and urinobacteria but decreases the proportion of deoxycholic acid. Moreover, HA can reduce liver and kidney injury and systemic inflammation caused by HS and reduce intestinal injury by enhancing the integrity of the intestinal barrier. In addition, HA regulates inflammation by inhibiting NF‐κB signalling and increasing tight junction protein expression in HS mice. HA induces changes in the gut microbiota, which may enhance tight junction protein expression, thereby reducing intestinal inflammation, promoting bile acid metabolism, and ultimately maintaining the integrity of the intestinal barrier. In conclusion, HA induced changes in the gut microbiota. Among the gut microbiota, lactobacilli may play a key role in the potential protective mechanism of HA. John Wiley and Sons Inc. 2023-10-04 /pmc/articles/PMC10616642/ /pubmed/37792264 http://dx.doi.org/10.1111/1751-7915.14349 Text en © 2023 The Authors. Microbial Biotechnology published by Applied Microbiology International and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Research Articles Huang, Xueyan Luo, Zhen Shen, Tingting He, Genlin Yu, Xueting Liu, Yishan Tan, Yulong Wang, Zeze Li, Ping Zhang, Boyi Zhou, Huan Chen, Xiangyu Wang, Ying Yu, Shasha Luo, Xue Yang, Xuesen Holistic view of heat acclimation alleviated intestinal lesion in mice with heat stroke based on microbiome‐metabolomics analysis |
title | Holistic view of heat acclimation alleviated intestinal lesion in mice with heat stroke based on microbiome‐metabolomics analysis |
title_full | Holistic view of heat acclimation alleviated intestinal lesion in mice with heat stroke based on microbiome‐metabolomics analysis |
title_fullStr | Holistic view of heat acclimation alleviated intestinal lesion in mice with heat stroke based on microbiome‐metabolomics analysis |
title_full_unstemmed | Holistic view of heat acclimation alleviated intestinal lesion in mice with heat stroke based on microbiome‐metabolomics analysis |
title_short | Holistic view of heat acclimation alleviated intestinal lesion in mice with heat stroke based on microbiome‐metabolomics analysis |
title_sort | holistic view of heat acclimation alleviated intestinal lesion in mice with heat stroke based on microbiome‐metabolomics analysis |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10616642/ https://www.ncbi.nlm.nih.gov/pubmed/37792264 http://dx.doi.org/10.1111/1751-7915.14349 |
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