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ILC3: a case of conflicted identity

Innate lymphoid cells type 3 (ILC3s) are the first line sentinels at the mucous tissues, where they contribute to the homeostatic immune response in a major way. Also, they have been increasingly appreciated as important modulators of chronic inflammatory and autoimmune responses, both locally and s...

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Autores principales: Koprivica, Ivan, Stanisavljević, Suzana, Mićanović, Dragica, Jevtić, Bojan, Stojanović, Ivana, Miljković, Đorđe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10616800/
https://www.ncbi.nlm.nih.gov/pubmed/37915588
http://dx.doi.org/10.3389/fimmu.2023.1271699
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author Koprivica, Ivan
Stanisavljević, Suzana
Mićanović, Dragica
Jevtić, Bojan
Stojanović, Ivana
Miljković, Đorđe
author_facet Koprivica, Ivan
Stanisavljević, Suzana
Mićanović, Dragica
Jevtić, Bojan
Stojanović, Ivana
Miljković, Đorđe
author_sort Koprivica, Ivan
collection PubMed
description Innate lymphoid cells type 3 (ILC3s) are the first line sentinels at the mucous tissues, where they contribute to the homeostatic immune response in a major way. Also, they have been increasingly appreciated as important modulators of chronic inflammatory and autoimmune responses, both locally and systemically. The proper identification of ILC3 is of utmost importance for meaningful studies on their role in immunity. Flow cytometry is the method of choice for the detection and characterization of ILC3. However, the analysis of ILC3-related papers shows inconsistency in ILC3 phenotypic definition, as different inclusion and exclusion markers are used for their identification. Here, we present these discrepancies in the phenotypic characterization of human and mouse ILC3s. We discuss the pros and cons of using various markers for ILC3 identification. Furthermore, we consider the possibilities for the efficient isolation and propagation of ILC3 from different organs and tissues for in-vitro and in-vivo studies. This paper calls upon uniformity in ILC3 definition, isolation, and propagation for the increased possibility of confluent interpretation of ILC3’s role in immunity.
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spelling pubmed-106168002023-11-01 ILC3: a case of conflicted identity Koprivica, Ivan Stanisavljević, Suzana Mićanović, Dragica Jevtić, Bojan Stojanović, Ivana Miljković, Đorđe Front Immunol Immunology Innate lymphoid cells type 3 (ILC3s) are the first line sentinels at the mucous tissues, where they contribute to the homeostatic immune response in a major way. Also, they have been increasingly appreciated as important modulators of chronic inflammatory and autoimmune responses, both locally and systemically. The proper identification of ILC3 is of utmost importance for meaningful studies on their role in immunity. Flow cytometry is the method of choice for the detection and characterization of ILC3. However, the analysis of ILC3-related papers shows inconsistency in ILC3 phenotypic definition, as different inclusion and exclusion markers are used for their identification. Here, we present these discrepancies in the phenotypic characterization of human and mouse ILC3s. We discuss the pros and cons of using various markers for ILC3 identification. Furthermore, we consider the possibilities for the efficient isolation and propagation of ILC3 from different organs and tissues for in-vitro and in-vivo studies. This paper calls upon uniformity in ILC3 definition, isolation, and propagation for the increased possibility of confluent interpretation of ILC3’s role in immunity. Frontiers Media S.A. 2023-10-17 /pmc/articles/PMC10616800/ /pubmed/37915588 http://dx.doi.org/10.3389/fimmu.2023.1271699 Text en Copyright © 2023 Koprivica, Stanisavljević, Mićanović, Jevtić, Stojanović and Miljković https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Koprivica, Ivan
Stanisavljević, Suzana
Mićanović, Dragica
Jevtić, Bojan
Stojanović, Ivana
Miljković, Đorđe
ILC3: a case of conflicted identity
title ILC3: a case of conflicted identity
title_full ILC3: a case of conflicted identity
title_fullStr ILC3: a case of conflicted identity
title_full_unstemmed ILC3: a case of conflicted identity
title_short ILC3: a case of conflicted identity
title_sort ilc3: a case of conflicted identity
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10616800/
https://www.ncbi.nlm.nih.gov/pubmed/37915588
http://dx.doi.org/10.3389/fimmu.2023.1271699
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