Cargando…
ILC3: a case of conflicted identity
Innate lymphoid cells type 3 (ILC3s) are the first line sentinels at the mucous tissues, where they contribute to the homeostatic immune response in a major way. Also, they have been increasingly appreciated as important modulators of chronic inflammatory and autoimmune responses, both locally and s...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10616800/ https://www.ncbi.nlm.nih.gov/pubmed/37915588 http://dx.doi.org/10.3389/fimmu.2023.1271699 |
_version_ | 1785129474092695552 |
---|---|
author | Koprivica, Ivan Stanisavljević, Suzana Mićanović, Dragica Jevtić, Bojan Stojanović, Ivana Miljković, Đorđe |
author_facet | Koprivica, Ivan Stanisavljević, Suzana Mićanović, Dragica Jevtić, Bojan Stojanović, Ivana Miljković, Đorđe |
author_sort | Koprivica, Ivan |
collection | PubMed |
description | Innate lymphoid cells type 3 (ILC3s) are the first line sentinels at the mucous tissues, where they contribute to the homeostatic immune response in a major way. Also, they have been increasingly appreciated as important modulators of chronic inflammatory and autoimmune responses, both locally and systemically. The proper identification of ILC3 is of utmost importance for meaningful studies on their role in immunity. Flow cytometry is the method of choice for the detection and characterization of ILC3. However, the analysis of ILC3-related papers shows inconsistency in ILC3 phenotypic definition, as different inclusion and exclusion markers are used for their identification. Here, we present these discrepancies in the phenotypic characterization of human and mouse ILC3s. We discuss the pros and cons of using various markers for ILC3 identification. Furthermore, we consider the possibilities for the efficient isolation and propagation of ILC3 from different organs and tissues for in-vitro and in-vivo studies. This paper calls upon uniformity in ILC3 definition, isolation, and propagation for the increased possibility of confluent interpretation of ILC3’s role in immunity. |
format | Online Article Text |
id | pubmed-10616800 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-106168002023-11-01 ILC3: a case of conflicted identity Koprivica, Ivan Stanisavljević, Suzana Mićanović, Dragica Jevtić, Bojan Stojanović, Ivana Miljković, Đorđe Front Immunol Immunology Innate lymphoid cells type 3 (ILC3s) are the first line sentinels at the mucous tissues, where they contribute to the homeostatic immune response in a major way. Also, they have been increasingly appreciated as important modulators of chronic inflammatory and autoimmune responses, both locally and systemically. The proper identification of ILC3 is of utmost importance for meaningful studies on their role in immunity. Flow cytometry is the method of choice for the detection and characterization of ILC3. However, the analysis of ILC3-related papers shows inconsistency in ILC3 phenotypic definition, as different inclusion and exclusion markers are used for their identification. Here, we present these discrepancies in the phenotypic characterization of human and mouse ILC3s. We discuss the pros and cons of using various markers for ILC3 identification. Furthermore, we consider the possibilities for the efficient isolation and propagation of ILC3 from different organs and tissues for in-vitro and in-vivo studies. This paper calls upon uniformity in ILC3 definition, isolation, and propagation for the increased possibility of confluent interpretation of ILC3’s role in immunity. Frontiers Media S.A. 2023-10-17 /pmc/articles/PMC10616800/ /pubmed/37915588 http://dx.doi.org/10.3389/fimmu.2023.1271699 Text en Copyright © 2023 Koprivica, Stanisavljević, Mićanović, Jevtić, Stojanović and Miljković https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Koprivica, Ivan Stanisavljević, Suzana Mićanović, Dragica Jevtić, Bojan Stojanović, Ivana Miljković, Đorđe ILC3: a case of conflicted identity |
title | ILC3: a case of conflicted identity |
title_full | ILC3: a case of conflicted identity |
title_fullStr | ILC3: a case of conflicted identity |
title_full_unstemmed | ILC3: a case of conflicted identity |
title_short | ILC3: a case of conflicted identity |
title_sort | ilc3: a case of conflicted identity |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10616800/ https://www.ncbi.nlm.nih.gov/pubmed/37915588 http://dx.doi.org/10.3389/fimmu.2023.1271699 |
work_keys_str_mv | AT koprivicaivan ilc3acaseofconflictedidentity AT stanisavljevicsuzana ilc3acaseofconflictedidentity AT micanovicdragica ilc3acaseofconflictedidentity AT jevticbojan ilc3acaseofconflictedidentity AT stojanovicivana ilc3acaseofconflictedidentity AT miljkovicđorđe ilc3acaseofconflictedidentity |