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MicroRNA-96-5p is negatively regulating GPC3 in the metastasis of papillary thyroid cancer
BACKGROUNDS: Papillary thyroid cancer is the most common pathological type of thyroid cancer. miR-96-5p, a member of the miR-183 family, constitute a polycistronic miRNA cluster. In breast cancer, miR-96-5p promotes cell invasion, migration, and proliferation in vitro by inhibiting PTPN9. Moreover,...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10617255/ https://www.ncbi.nlm.nih.gov/pubmed/37915840 http://dx.doi.org/10.1177/20503121231205710 |
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author | Hu, Haibei Quan, Guangqian Yang, Feng Du, Shan Ding, Siqin Lun, Yongzhi Chen, Qiang |
author_facet | Hu, Haibei Quan, Guangqian Yang, Feng Du, Shan Ding, Siqin Lun, Yongzhi Chen, Qiang |
author_sort | Hu, Haibei |
collection | PubMed |
description | BACKGROUNDS: Papillary thyroid cancer is the most common pathological type of thyroid cancer. miR-96-5p, a member of the miR-183 family, constitute a polycistronic miRNA cluster. In breast cancer, miR-96-5p promotes cell invasion, migration, and proliferation in vitro by inhibiting PTPN9. Moreover, miR-96-5p was reported to function as an oncogene in many cancers. However, whether miR-96-5p is involved in the development of papillary thyroid cancers and its potential mechanism is still unknown. The present study aims to explore the relationship between miR-96-5p and GPC3 expression in the development of papillary thyroid cancers. METHODS: Transcriptomic sequencing was carried out using six pairs of papillary thyroid cancer and adjacent normal tissues. Quantitative real-time polymerase chain reaction (PCR) experiments were performed to examine the expression of genes. RESULTS: In total, there were 1588 up-regulated and 1803 down-regulated differentially expressed genes between papillary thyroid cancer and normal tissues. Gene ontology and Kyoto encyclopedia of genes and genomes analysis revealed that extracellular matrix structure and proteoglycans were mainly involved in papillary thyroid cancer. Among the cluster of proteoglycans, GPC3 was significantly down-regulated in papillary thyroid cancer and is a target of miR-96. CONCLUSION: miR-96-5p participates in the development of papillary thyroid cancer by regulating the expression of GPC3. Thus, targeting miR-96-5p may be a potential therapeutic approach for preventing and treating papillary thyroid cancer. |
format | Online Article Text |
id | pubmed-10617255 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-106172552023-11-01 MicroRNA-96-5p is negatively regulating GPC3 in the metastasis of papillary thyroid cancer Hu, Haibei Quan, Guangqian Yang, Feng Du, Shan Ding, Siqin Lun, Yongzhi Chen, Qiang SAGE Open Med Original Article BACKGROUNDS: Papillary thyroid cancer is the most common pathological type of thyroid cancer. miR-96-5p, a member of the miR-183 family, constitute a polycistronic miRNA cluster. In breast cancer, miR-96-5p promotes cell invasion, migration, and proliferation in vitro by inhibiting PTPN9. Moreover, miR-96-5p was reported to function as an oncogene in many cancers. However, whether miR-96-5p is involved in the development of papillary thyroid cancers and its potential mechanism is still unknown. The present study aims to explore the relationship between miR-96-5p and GPC3 expression in the development of papillary thyroid cancers. METHODS: Transcriptomic sequencing was carried out using six pairs of papillary thyroid cancer and adjacent normal tissues. Quantitative real-time polymerase chain reaction (PCR) experiments were performed to examine the expression of genes. RESULTS: In total, there were 1588 up-regulated and 1803 down-regulated differentially expressed genes between papillary thyroid cancer and normal tissues. Gene ontology and Kyoto encyclopedia of genes and genomes analysis revealed that extracellular matrix structure and proteoglycans were mainly involved in papillary thyroid cancer. Among the cluster of proteoglycans, GPC3 was significantly down-regulated in papillary thyroid cancer and is a target of miR-96. CONCLUSION: miR-96-5p participates in the development of papillary thyroid cancer by regulating the expression of GPC3. Thus, targeting miR-96-5p may be a potential therapeutic approach for preventing and treating papillary thyroid cancer. SAGE Publications 2023-10-29 /pmc/articles/PMC10617255/ /pubmed/37915840 http://dx.doi.org/10.1177/20503121231205710 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Original Article Hu, Haibei Quan, Guangqian Yang, Feng Du, Shan Ding, Siqin Lun, Yongzhi Chen, Qiang MicroRNA-96-5p is negatively regulating GPC3 in the metastasis of papillary thyroid cancer |
title | MicroRNA-96-5p is negatively regulating GPC3 in the metastasis of papillary thyroid cancer |
title_full | MicroRNA-96-5p is negatively regulating GPC3 in the metastasis of papillary thyroid cancer |
title_fullStr | MicroRNA-96-5p is negatively regulating GPC3 in the metastasis of papillary thyroid cancer |
title_full_unstemmed | MicroRNA-96-5p is negatively regulating GPC3 in the metastasis of papillary thyroid cancer |
title_short | MicroRNA-96-5p is negatively regulating GPC3 in the metastasis of papillary thyroid cancer |
title_sort | microrna-96-5p is negatively regulating gpc3 in the metastasis of papillary thyroid cancer |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10617255/ https://www.ncbi.nlm.nih.gov/pubmed/37915840 http://dx.doi.org/10.1177/20503121231205710 |
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