Cargando…

A standardized and efficient technique to estimate seed traits in plants with numerous small propagules

PREMISE: Variation in seed traits is common within and among populations of plant species and often has ecological and evolutionary implications. However, due to the time‐consuming nature of manual seed measurements and the level of variability in imaging techniques, quantifying and interpreting the...

Descripción completa

Detalles Bibliográficos
Autores principales: Steinecke, Christina, Lee, Jeremiah, Friedman, Jannice
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10617364/
https://www.ncbi.nlm.nih.gov/pubmed/37915429
http://dx.doi.org/10.1002/aps3.11552
_version_ 1785129595033354240
author Steinecke, Christina
Lee, Jeremiah
Friedman, Jannice
author_facet Steinecke, Christina
Lee, Jeremiah
Friedman, Jannice
author_sort Steinecke, Christina
collection PubMed
description PREMISE: Variation in seed traits is common within and among populations of plant species and often has ecological and evolutionary implications. However, due to the time‐consuming nature of manual seed measurements and the level of variability in imaging techniques, quantifying and interpreting the extent of seed variation can be challenging. METHODS: We developed a standardized high‐throughput technique to measure seed number, as well as individual seed area and color, using a derived empirical scale to constrain area in Arabidopsis thaliana, Brassica rapa, and Mimulus guttatus. We develop a specific rational model using seed area measured at various spatial scales relative to the pixel count, observing the asymptotic value of the seed area as the modeled number of pixels approaches infinity. RESULTS: We found that our model has high reliability in estimating seed traits and efficiently processes large numbers of images, facilitating the quantification of seed traits in studies with large sample sizes. DISCUSSION: This technique facilitates consistency between imaging sessions and standardizes the measurement of seed traits. These novel advances allow researchers to directly and reliably measure seed traits, which will enable tests of the ecological and evolutionary causes of their variation.
format Online
Article
Text
id pubmed-10617364
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-106173642023-11-01 A standardized and efficient technique to estimate seed traits in plants with numerous small propagules Steinecke, Christina Lee, Jeremiah Friedman, Jannice Appl Plant Sci Application Articles PREMISE: Variation in seed traits is common within and among populations of plant species and often has ecological and evolutionary implications. However, due to the time‐consuming nature of manual seed measurements and the level of variability in imaging techniques, quantifying and interpreting the extent of seed variation can be challenging. METHODS: We developed a standardized high‐throughput technique to measure seed number, as well as individual seed area and color, using a derived empirical scale to constrain area in Arabidopsis thaliana, Brassica rapa, and Mimulus guttatus. We develop a specific rational model using seed area measured at various spatial scales relative to the pixel count, observing the asymptotic value of the seed area as the modeled number of pixels approaches infinity. RESULTS: We found that our model has high reliability in estimating seed traits and efficiently processes large numbers of images, facilitating the quantification of seed traits in studies with large sample sizes. DISCUSSION: This technique facilitates consistency between imaging sessions and standardizes the measurement of seed traits. These novel advances allow researchers to directly and reliably measure seed traits, which will enable tests of the ecological and evolutionary causes of their variation. John Wiley and Sons Inc. 2023-10-31 /pmc/articles/PMC10617364/ /pubmed/37915429 http://dx.doi.org/10.1002/aps3.11552 Text en © 2023 The Authors. Applications in Plant Sciences published by Wiley Periodicals LLC on behalf of Botanical Society of America. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Application Articles
Steinecke, Christina
Lee, Jeremiah
Friedman, Jannice
A standardized and efficient technique to estimate seed traits in plants with numerous small propagules
title A standardized and efficient technique to estimate seed traits in plants with numerous small propagules
title_full A standardized and efficient technique to estimate seed traits in plants with numerous small propagules
title_fullStr A standardized and efficient technique to estimate seed traits in plants with numerous small propagules
title_full_unstemmed A standardized and efficient technique to estimate seed traits in plants with numerous small propagules
title_short A standardized and efficient technique to estimate seed traits in plants with numerous small propagules
title_sort standardized and efficient technique to estimate seed traits in plants with numerous small propagules
topic Application Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10617364/
https://www.ncbi.nlm.nih.gov/pubmed/37915429
http://dx.doi.org/10.1002/aps3.11552
work_keys_str_mv AT steineckechristina astandardizedandefficienttechniquetoestimateseedtraitsinplantswithnumeroussmallpropagules
AT leejeremiah astandardizedandefficienttechniquetoestimateseedtraitsinplantswithnumeroussmallpropagules
AT friedmanjannice astandardizedandefficienttechniquetoestimateseedtraitsinplantswithnumeroussmallpropagules
AT steineckechristina standardizedandefficienttechniquetoestimateseedtraitsinplantswithnumeroussmallpropagules
AT leejeremiah standardizedandefficienttechniquetoestimateseedtraitsinplantswithnumeroussmallpropagules
AT friedmanjannice standardizedandefficienttechniquetoestimateseedtraitsinplantswithnumeroussmallpropagules