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Oncogene SCARNA12 as a potential diagnostic biomarker for colorectal cancer

Colorectal cancer (CRC) is one of the most common malignant tumors of the digestive system, and represents a severe threat to the life and health of individuals. Increasing evidence supports the role of small nucleolar RNAs (snoRNAs) as critical regulatory gene in cancer development. Small Cajal bod...

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Autores principales: Zhang, Hong, Liu, Xin, Zhang, Wencheng, Deng, Jiarong, Lin, Chuxian, Qi, Zhenhua, Li, Yaqiong, Gu, Yongqing, Wang, Qi, Shen, Liping, Wang, Zhidong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Nature Singapore 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10618143/
https://www.ncbi.nlm.nih.gov/pubmed/37907779
http://dx.doi.org/10.1186/s43556-023-00147-x
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author Zhang, Hong
Liu, Xin
Zhang, Wencheng
Deng, Jiarong
Lin, Chuxian
Qi, Zhenhua
Li, Yaqiong
Gu, Yongqing
Wang, Qi
Shen, Liping
Wang, Zhidong
author_facet Zhang, Hong
Liu, Xin
Zhang, Wencheng
Deng, Jiarong
Lin, Chuxian
Qi, Zhenhua
Li, Yaqiong
Gu, Yongqing
Wang, Qi
Shen, Liping
Wang, Zhidong
author_sort Zhang, Hong
collection PubMed
description Colorectal cancer (CRC) is one of the most common malignant tumors of the digestive system, and represents a severe threat to the life and health of individuals. Increasing evidence supports the role of small nucleolar RNAs (snoRNAs) as critical regulatory gene in cancer development. Small Cajal body-specific RNAs (scaRNAs), a subtype of snoRNAs, are named for their subcellular localization within Cajal bodies. SCARNA12, which located at the intronic region of PHB2 in chromosome 12p13.31 with 270 nucleotides (nt) in length. It has been reported function as a diagnostic marker for cervical cancer. However, its biological functions and molecular mechanisms in CRC have yet to be elucidated. In this study, bioinformatics analysis revealed that SCARNA12 was highly expressed in CRC and positively correlated with poor prognosis in CRC patients. Additionally, SCARNA12 showed upregulated expression in CRC cell lines and clinical CRC tissue samples. Moreover, SCARNA12 overexpression in SW620 cells accelerated cell proliferation, suppressed the apoptosis rate, and enhanced tumorigenesis in vivo. The knockdown of SCARNA12 expression in HCT116 and HT29 cells resulted in contrasting effects. The functioning of SCARNA12 is mechanically independent of its host gene PHB2. Notably, the overexpression of SCARNA12 activated PI3K/AKT pathway in SW620 cells, and the malignancy degree of CRC cells was attenuated after treatment with MK2206 (a specific AKT inhibitor). Our findings demonstrated that SCARNA12 plays an oncogenic role in CRC progression and can be used as a potential diagnostic biomarker for CRC. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s43556-023-00147-x.
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spelling pubmed-106181432023-11-02 Oncogene SCARNA12 as a potential diagnostic biomarker for colorectal cancer Zhang, Hong Liu, Xin Zhang, Wencheng Deng, Jiarong Lin, Chuxian Qi, Zhenhua Li, Yaqiong Gu, Yongqing Wang, Qi Shen, Liping Wang, Zhidong Mol Biomed Research Colorectal cancer (CRC) is one of the most common malignant tumors of the digestive system, and represents a severe threat to the life and health of individuals. Increasing evidence supports the role of small nucleolar RNAs (snoRNAs) as critical regulatory gene in cancer development. Small Cajal body-specific RNAs (scaRNAs), a subtype of snoRNAs, are named for their subcellular localization within Cajal bodies. SCARNA12, which located at the intronic region of PHB2 in chromosome 12p13.31 with 270 nucleotides (nt) in length. It has been reported function as a diagnostic marker for cervical cancer. However, its biological functions and molecular mechanisms in CRC have yet to be elucidated. In this study, bioinformatics analysis revealed that SCARNA12 was highly expressed in CRC and positively correlated with poor prognosis in CRC patients. Additionally, SCARNA12 showed upregulated expression in CRC cell lines and clinical CRC tissue samples. Moreover, SCARNA12 overexpression in SW620 cells accelerated cell proliferation, suppressed the apoptosis rate, and enhanced tumorigenesis in vivo. The knockdown of SCARNA12 expression in HCT116 and HT29 cells resulted in contrasting effects. The functioning of SCARNA12 is mechanically independent of its host gene PHB2. Notably, the overexpression of SCARNA12 activated PI3K/AKT pathway in SW620 cells, and the malignancy degree of CRC cells was attenuated after treatment with MK2206 (a specific AKT inhibitor). Our findings demonstrated that SCARNA12 plays an oncogenic role in CRC progression and can be used as a potential diagnostic biomarker for CRC. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s43556-023-00147-x. Springer Nature Singapore 2023-11-01 /pmc/articles/PMC10618143/ /pubmed/37907779 http://dx.doi.org/10.1186/s43556-023-00147-x Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research
Zhang, Hong
Liu, Xin
Zhang, Wencheng
Deng, Jiarong
Lin, Chuxian
Qi, Zhenhua
Li, Yaqiong
Gu, Yongqing
Wang, Qi
Shen, Liping
Wang, Zhidong
Oncogene SCARNA12 as a potential diagnostic biomarker for colorectal cancer
title Oncogene SCARNA12 as a potential diagnostic biomarker for colorectal cancer
title_full Oncogene SCARNA12 as a potential diagnostic biomarker for colorectal cancer
title_fullStr Oncogene SCARNA12 as a potential diagnostic biomarker for colorectal cancer
title_full_unstemmed Oncogene SCARNA12 as a potential diagnostic biomarker for colorectal cancer
title_short Oncogene SCARNA12 as a potential diagnostic biomarker for colorectal cancer
title_sort oncogene scarna12 as a potential diagnostic biomarker for colorectal cancer
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10618143/
https://www.ncbi.nlm.nih.gov/pubmed/37907779
http://dx.doi.org/10.1186/s43556-023-00147-x
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