Cargando…

Pyrethroid insecticides susceptibility profiles and evaluation of L1014F kdr mutant alleles in Culex quinquefasciatus from lymphatic filariasis endemic communities

This study investigated the dynamics in pyrethriod resistance and the presence/frequencies of L1014F knockdown resistance mutant allelles in Culex quinquefasciatus vector populations from Uruan Local Government Area of AkwaIbom State, Southern Nigeria between the months of March and November, 2021....

Descripción completa

Detalles Bibliográficos
Autores principales: Okafor, Martina Anurika, Ekpo, Ndifreke Daniel, Opara, Kenneth Nnamdi, Udoidung, Nsima Ibanga, Ataya, Farid S., Yaro, Clement Ameh, Batiha, Gaber El-Saber, Alexiou, Athanasios, Papadakis, Marios
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10618241/
https://www.ncbi.nlm.nih.gov/pubmed/37907533
http://dx.doi.org/10.1038/s41598-023-44962-2
_version_ 1785129731906076672
author Okafor, Martina Anurika
Ekpo, Ndifreke Daniel
Opara, Kenneth Nnamdi
Udoidung, Nsima Ibanga
Ataya, Farid S.
Yaro, Clement Ameh
Batiha, Gaber El-Saber
Alexiou, Athanasios
Papadakis, Marios
author_facet Okafor, Martina Anurika
Ekpo, Ndifreke Daniel
Opara, Kenneth Nnamdi
Udoidung, Nsima Ibanga
Ataya, Farid S.
Yaro, Clement Ameh
Batiha, Gaber El-Saber
Alexiou, Athanasios
Papadakis, Marios
author_sort Okafor, Martina Anurika
collection PubMed
description This study investigated the dynamics in pyrethriod resistance and the presence/frequencies of L1014F knockdown resistance mutant allelles in Culex quinquefasciatus vector populations from Uruan Local Government Area of AkwaIbom State, Southern Nigeria between the months of March and November, 2021. Uruan LGA is among the endemic LGAs for lymphatic filariasis in AkwaIbomState. Female Anopheles mosquitoes from Eman Uruan, Ituk Mbang and Idu Uruan were exposed to permethrin, deltamethrin and alphacypermethrin in CDC insecticide coated bottles for susceptibility bioassay following standard protocols. The mosquitoes were obtained as aquatic forms from the study sites and reared under laboratory conditions to adults. The adult mosquitoes were used for this study. All the mosquitoes used for the insecticide susceptibility bioassay were morphologically identified. Standard Polymerase chain reaction (PCR) was used for authenticating the Culex quinquefasciatus species. A portion of the vgsc (917 bp) gene spanning the entire intron and the exon containing the L1014F mutation associated with knockdown resistance (kdr) in the vectorswere amplified using Allele-SPECIFIC POLYMERASE CHAIN REACTION (AS-PCR) in order to detect target site insensitivity in the vectors from the study sites. Results obtained revealed that vectors from all the study sites were resistant to permethrin insecticide (mortality rate: 18–23%). Suspected resistance (mortality rate: 90–93%) to deltamethrin and low resistance (mortality rate: 82–85%) to alphacypermethrin insecticides were detected. knockdown was more rapid with deltamethrin and alphacypermethrin than with permethrin across the study sites considering their KDT(50) and KDT(95). The frequency of the resistant phenotypes ranged from 35.14 to 55.3% across the study sites with a net of 45.1% resistant phenotype recorded in this study. The 1014F allelic frequency calculated from Hardy–Weinberg principle for vector populations across the study sites ranged from 0.500 (50.00%) to 0.7763 (77.63%). All populations witnessed significant (p < 0.05) deviations from Hardy–Weinberg equilibrium in the distribution of these alleles. The findings of this study show that there is a tendency to record an entire population of resistant vectors in this study area over time due to natural selection. The public health implication of these findings is that the use of pyrethroid based aerosols, coils, sprays, LLITNs and others for the purpose of controlling vectors of lymphatic filariasis and other diseases may be effort in futility.
format Online
Article
Text
id pubmed-10618241
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-106182412023-11-02 Pyrethroid insecticides susceptibility profiles and evaluation of L1014F kdr mutant alleles in Culex quinquefasciatus from lymphatic filariasis endemic communities Okafor, Martina Anurika Ekpo, Ndifreke Daniel Opara, Kenneth Nnamdi Udoidung, Nsima Ibanga Ataya, Farid S. Yaro, Clement Ameh Batiha, Gaber El-Saber Alexiou, Athanasios Papadakis, Marios Sci Rep Article This study investigated the dynamics in pyrethriod resistance and the presence/frequencies of L1014F knockdown resistance mutant allelles in Culex quinquefasciatus vector populations from Uruan Local Government Area of AkwaIbom State, Southern Nigeria between the months of March and November, 2021. Uruan LGA is among the endemic LGAs for lymphatic filariasis in AkwaIbomState. Female Anopheles mosquitoes from Eman Uruan, Ituk Mbang and Idu Uruan were exposed to permethrin, deltamethrin and alphacypermethrin in CDC insecticide coated bottles for susceptibility bioassay following standard protocols. The mosquitoes were obtained as aquatic forms from the study sites and reared under laboratory conditions to adults. The adult mosquitoes were used for this study. All the mosquitoes used for the insecticide susceptibility bioassay were morphologically identified. Standard Polymerase chain reaction (PCR) was used for authenticating the Culex quinquefasciatus species. A portion of the vgsc (917 bp) gene spanning the entire intron and the exon containing the L1014F mutation associated with knockdown resistance (kdr) in the vectorswere amplified using Allele-SPECIFIC POLYMERASE CHAIN REACTION (AS-PCR) in order to detect target site insensitivity in the vectors from the study sites. Results obtained revealed that vectors from all the study sites were resistant to permethrin insecticide (mortality rate: 18–23%). Suspected resistance (mortality rate: 90–93%) to deltamethrin and low resistance (mortality rate: 82–85%) to alphacypermethrin insecticides were detected. knockdown was more rapid with deltamethrin and alphacypermethrin than with permethrin across the study sites considering their KDT(50) and KDT(95). The frequency of the resistant phenotypes ranged from 35.14 to 55.3% across the study sites with a net of 45.1% resistant phenotype recorded in this study. The 1014F allelic frequency calculated from Hardy–Weinberg principle for vector populations across the study sites ranged from 0.500 (50.00%) to 0.7763 (77.63%). All populations witnessed significant (p < 0.05) deviations from Hardy–Weinberg equilibrium in the distribution of these alleles. The findings of this study show that there is a tendency to record an entire population of resistant vectors in this study area over time due to natural selection. The public health implication of these findings is that the use of pyrethroid based aerosols, coils, sprays, LLITNs and others for the purpose of controlling vectors of lymphatic filariasis and other diseases may be effort in futility. Nature Publishing Group UK 2023-10-31 /pmc/articles/PMC10618241/ /pubmed/37907533 http://dx.doi.org/10.1038/s41598-023-44962-2 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Okafor, Martina Anurika
Ekpo, Ndifreke Daniel
Opara, Kenneth Nnamdi
Udoidung, Nsima Ibanga
Ataya, Farid S.
Yaro, Clement Ameh
Batiha, Gaber El-Saber
Alexiou, Athanasios
Papadakis, Marios
Pyrethroid insecticides susceptibility profiles and evaluation of L1014F kdr mutant alleles in Culex quinquefasciatus from lymphatic filariasis endemic communities
title Pyrethroid insecticides susceptibility profiles and evaluation of L1014F kdr mutant alleles in Culex quinquefasciatus from lymphatic filariasis endemic communities
title_full Pyrethroid insecticides susceptibility profiles and evaluation of L1014F kdr mutant alleles in Culex quinquefasciatus from lymphatic filariasis endemic communities
title_fullStr Pyrethroid insecticides susceptibility profiles and evaluation of L1014F kdr mutant alleles in Culex quinquefasciatus from lymphatic filariasis endemic communities
title_full_unstemmed Pyrethroid insecticides susceptibility profiles and evaluation of L1014F kdr mutant alleles in Culex quinquefasciatus from lymphatic filariasis endemic communities
title_short Pyrethroid insecticides susceptibility profiles and evaluation of L1014F kdr mutant alleles in Culex quinquefasciatus from lymphatic filariasis endemic communities
title_sort pyrethroid insecticides susceptibility profiles and evaluation of l1014f kdr mutant alleles in culex quinquefasciatus from lymphatic filariasis endemic communities
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10618241/
https://www.ncbi.nlm.nih.gov/pubmed/37907533
http://dx.doi.org/10.1038/s41598-023-44962-2
work_keys_str_mv AT okaformartinaanurika pyrethroidinsecticidessusceptibilityprofilesandevaluationofl1014fkdrmutantallelesinculexquinquefasciatusfromlymphaticfilariasisendemiccommunities
AT ekpondifrekedaniel pyrethroidinsecticidessusceptibilityprofilesandevaluationofl1014fkdrmutantallelesinculexquinquefasciatusfromlymphaticfilariasisendemiccommunities
AT oparakennethnnamdi pyrethroidinsecticidessusceptibilityprofilesandevaluationofl1014fkdrmutantallelesinculexquinquefasciatusfromlymphaticfilariasisendemiccommunities
AT udoidungnsimaibanga pyrethroidinsecticidessusceptibilityprofilesandevaluationofl1014fkdrmutantallelesinculexquinquefasciatusfromlymphaticfilariasisendemiccommunities
AT atayafarids pyrethroidinsecticidessusceptibilityprofilesandevaluationofl1014fkdrmutantallelesinculexquinquefasciatusfromlymphaticfilariasisendemiccommunities
AT yaroclementameh pyrethroidinsecticidessusceptibilityprofilesandevaluationofl1014fkdrmutantallelesinculexquinquefasciatusfromlymphaticfilariasisendemiccommunities
AT batihagaberelsaber pyrethroidinsecticidessusceptibilityprofilesandevaluationofl1014fkdrmutantallelesinculexquinquefasciatusfromlymphaticfilariasisendemiccommunities
AT alexiouathanasios pyrethroidinsecticidessusceptibilityprofilesandevaluationofl1014fkdrmutantallelesinculexquinquefasciatusfromlymphaticfilariasisendemiccommunities
AT papadakismarios pyrethroidinsecticidessusceptibilityprofilesandevaluationofl1014fkdrmutantallelesinculexquinquefasciatusfromlymphaticfilariasisendemiccommunities