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The H9c2(2-1) cell-based sulforhodamine B assay is a non-animal alternative to evaluate municipal wastewater quality over time

The present study validates the potential of the in vitro H9c2(2-1) cell-based sulforhodamine B (SRB) assay to evaluate the temporal variability of wastewater quality. The impact of effluent disposal on water quality and the efficiency of the wastewater treatment process were also assessed. To corre...

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Detalles Bibliográficos
Autores principales: Rodrigues, Elsa T., Pereira, Eduarda, Oliveira, Paulo J., Pardal, Miguel A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10618365/
https://www.ncbi.nlm.nih.gov/pubmed/37906309
http://dx.doi.org/10.1007/s10661-023-12017-8
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author Rodrigues, Elsa T.
Pereira, Eduarda
Oliveira, Paulo J.
Pardal, Miguel A.
author_facet Rodrigues, Elsa T.
Pereira, Eduarda
Oliveira, Paulo J.
Pardal, Miguel A.
author_sort Rodrigues, Elsa T.
collection PubMed
description The present study validates the potential of the in vitro H9c2(2-1) cell-based sulforhodamine B (SRB) assay to evaluate the temporal variability of wastewater quality. The impact of effluent disposal on water quality and the efficiency of the wastewater treatment process were also assessed. To correlate standard analytical method results with in vitro results, a total of 16 physicochemical parameters, such as nutrients, pH, chemical oxygen demand, total suspended solids and metals, were determined in both raw and treated wastewater samples. Results revealed that the H9c2(2-1) cell-based SRB assay has an enormous potential to evaluate municipal wastewater quality over time and to discriminate influent and effluent toxic characteristics, as well as for water quality monitoring and surveillance of the efficacy of treatment processes. Finally, the gathered results alerted to the impact of phosphates in a biological system, leading us to recommend the selection of this parameter as a potential environmental health indicator. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10661-023-12017-8.
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spelling pubmed-106183652023-11-02 The H9c2(2-1) cell-based sulforhodamine B assay is a non-animal alternative to evaluate municipal wastewater quality over time Rodrigues, Elsa T. Pereira, Eduarda Oliveira, Paulo J. Pardal, Miguel A. Environ Monit Assess Research The present study validates the potential of the in vitro H9c2(2-1) cell-based sulforhodamine B (SRB) assay to evaluate the temporal variability of wastewater quality. The impact of effluent disposal on water quality and the efficiency of the wastewater treatment process were also assessed. To correlate standard analytical method results with in vitro results, a total of 16 physicochemical parameters, such as nutrients, pH, chemical oxygen demand, total suspended solids and metals, were determined in both raw and treated wastewater samples. Results revealed that the H9c2(2-1) cell-based SRB assay has an enormous potential to evaluate municipal wastewater quality over time and to discriminate influent and effluent toxic characteristics, as well as for water quality monitoring and surveillance of the efficacy of treatment processes. Finally, the gathered results alerted to the impact of phosphates in a biological system, leading us to recommend the selection of this parameter as a potential environmental health indicator. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10661-023-12017-8. Springer International Publishing 2023-10-31 2023 /pmc/articles/PMC10618365/ /pubmed/37906309 http://dx.doi.org/10.1007/s10661-023-12017-8 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research
Rodrigues, Elsa T.
Pereira, Eduarda
Oliveira, Paulo J.
Pardal, Miguel A.
The H9c2(2-1) cell-based sulforhodamine B assay is a non-animal alternative to evaluate municipal wastewater quality over time
title The H9c2(2-1) cell-based sulforhodamine B assay is a non-animal alternative to evaluate municipal wastewater quality over time
title_full The H9c2(2-1) cell-based sulforhodamine B assay is a non-animal alternative to evaluate municipal wastewater quality over time
title_fullStr The H9c2(2-1) cell-based sulforhodamine B assay is a non-animal alternative to evaluate municipal wastewater quality over time
title_full_unstemmed The H9c2(2-1) cell-based sulforhodamine B assay is a non-animal alternative to evaluate municipal wastewater quality over time
title_short The H9c2(2-1) cell-based sulforhodamine B assay is a non-animal alternative to evaluate municipal wastewater quality over time
title_sort h9c2(2-1) cell-based sulforhodamine b assay is a non-animal alternative to evaluate municipal wastewater quality over time
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10618365/
https://www.ncbi.nlm.nih.gov/pubmed/37906309
http://dx.doi.org/10.1007/s10661-023-12017-8
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