A strategy for orthogonal deubiquitination using a bump-and-hole approach

We have successfully applied a bump-and-hole approach to establish orthogonal deubiquitination in which a ubiquitin substrate variant is specifically targeted by an engineered deubiquitinating enzyme (DUB). This makes it possibe to selectively observe and measure a single type of DUB activity in liv...

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Detalles Bibliográficos
Autores principales: Suzuki, Takumi, Utsugi, Yuki, Yamanaka, Satoshi, Takahashi, Hirotaka, Sato, Yusuke, Sawasaki, Tatsuya, Miyamae, Yusaku
Formato: Online Artículo Texto
Lenguaje:English
Publicado: RSC 2023
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10619139/
https://www.ncbi.nlm.nih.gov/pubmed/37920396
http://dx.doi.org/10.1039/d3cb00095h
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author Suzuki, Takumi
Utsugi, Yuki
Yamanaka, Satoshi
Takahashi, Hirotaka
Sato, Yusuke
Sawasaki, Tatsuya
Miyamae, Yusaku
author_facet Suzuki, Takumi
Utsugi, Yuki
Yamanaka, Satoshi
Takahashi, Hirotaka
Sato, Yusuke
Sawasaki, Tatsuya
Miyamae, Yusaku
author_sort Suzuki, Takumi
collection PubMed
description We have successfully applied a bump-and-hole approach to establish orthogonal deubiquitination in which a ubiquitin substrate variant is specifically targeted by an engineered deubiquitinating enzyme (DUB). This makes it possibe to selectively observe and measure a single type of DUB activity in living cells.
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spelling pubmed-106191392023-11-02 A strategy for orthogonal deubiquitination using a bump-and-hole approach Suzuki, Takumi Utsugi, Yuki Yamanaka, Satoshi Takahashi, Hirotaka Sato, Yusuke Sawasaki, Tatsuya Miyamae, Yusaku RSC Chem Biol Chemistry We have successfully applied a bump-and-hole approach to establish orthogonal deubiquitination in which a ubiquitin substrate variant is specifically targeted by an engineered deubiquitinating enzyme (DUB). This makes it possibe to selectively observe and measure a single type of DUB activity in living cells. RSC 2023-10-02 /pmc/articles/PMC10619139/ /pubmed/37920396 http://dx.doi.org/10.1039/d3cb00095h Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Suzuki, Takumi
Utsugi, Yuki
Yamanaka, Satoshi
Takahashi, Hirotaka
Sato, Yusuke
Sawasaki, Tatsuya
Miyamae, Yusaku
A strategy for orthogonal deubiquitination using a bump-and-hole approach
title A strategy for orthogonal deubiquitination using a bump-and-hole approach
title_full A strategy for orthogonal deubiquitination using a bump-and-hole approach
title_fullStr A strategy for orthogonal deubiquitination using a bump-and-hole approach
title_full_unstemmed A strategy for orthogonal deubiquitination using a bump-and-hole approach
title_short A strategy for orthogonal deubiquitination using a bump-and-hole approach
title_sort strategy for orthogonal deubiquitination using a bump-and-hole approach
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10619139/
https://www.ncbi.nlm.nih.gov/pubmed/37920396
http://dx.doi.org/10.1039/d3cb00095h
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