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Advantages and challenges associated with bisulfite-assisted nanopore direct RNA sequencing for modifications

Nanopore direct RNA sequencing is a technology that allows sequencing for epitranscriptomic modifications with the possibility of a quantitative assessment. In the present work, pseudouridine (Ψ) was sequenced with the nanopore before and after the pH 7 bisulfite reaction that yields stable ribose a...

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Detalles Bibliográficos
Autores principales:	Fleming, Aaron M., Zhu, Judy, Done, Vilhelmina K., Burrows, Cynthia J.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	RSC 2023
Materias:	Chemistry
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10619145/ https://www.ncbi.nlm.nih.gov/pubmed/37920399 http://dx.doi.org/10.1039/d3cb00081h

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