Cargando…
Anaerobic fixed-target serial crystallography using sandwiched silicon nitride membranes
In recent years, the emergence of serial crystallography, initially pioneered at X-ray free-electron lasers (XFELs), has sparked a growing interest in collecting macromolecular crystallographic data at room temperature. Various fixed-target serial crystallography techniques have been developed, rang...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Union of Crystallography
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10619425/ https://www.ncbi.nlm.nih.gov/pubmed/37860963 http://dx.doi.org/10.1107/S205979832300880X |
_version_ | 1785129987042443264 |
---|---|
author | Bjelčić, Monika Sigfridsson Clauss, Kajsa G. V. Aurelius, Oskar Milas, Mirko Nan, Jie Ursby, Thomas |
author_facet | Bjelčić, Monika Sigfridsson Clauss, Kajsa G. V. Aurelius, Oskar Milas, Mirko Nan, Jie Ursby, Thomas |
author_sort | Bjelčić, Monika |
collection | PubMed |
description | In recent years, the emergence of serial crystallography, initially pioneered at X-ray free-electron lasers (XFELs), has sparked a growing interest in collecting macromolecular crystallographic data at room temperature. Various fixed-target serial crystallography techniques have been developed, ranging from commercially available chips to in-house designs implemented at different synchrotron facilities. Nevertheless, there is currently no commercially available chip (known to the authors) specifically designed for the direct handling of oxygen-sensitive samples. This study presents a methodology employing silicon nitride chips arranged in a ‘sandwich’ configuration, enabling reliable room-temperature data collection from oxygen-sensitive samples. The method involves the utilization of a custom-made 3D-printed assembling tool and a MX sample holder. To validate the effectiveness of the proposed method, deoxyhemoglobin and methemoglobin samples were investigated using the BioMAX X-ray macromolecular crystallography beamline, the Balder X-ray absorption spectroscopy beamline and UV–Vis absorption spectroscopy. |
format | Online Article Text |
id | pubmed-10619425 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | International Union of Crystallography |
record_format | MEDLINE/PubMed |
spelling | pubmed-106194252023-11-02 Anaerobic fixed-target serial crystallography using sandwiched silicon nitride membranes Bjelčić, Monika Sigfridsson Clauss, Kajsa G. V. Aurelius, Oskar Milas, Mirko Nan, Jie Ursby, Thomas Acta Crystallogr D Struct Biol Research Papers In recent years, the emergence of serial crystallography, initially pioneered at X-ray free-electron lasers (XFELs), has sparked a growing interest in collecting macromolecular crystallographic data at room temperature. Various fixed-target serial crystallography techniques have been developed, ranging from commercially available chips to in-house designs implemented at different synchrotron facilities. Nevertheless, there is currently no commercially available chip (known to the authors) specifically designed for the direct handling of oxygen-sensitive samples. This study presents a methodology employing silicon nitride chips arranged in a ‘sandwich’ configuration, enabling reliable room-temperature data collection from oxygen-sensitive samples. The method involves the utilization of a custom-made 3D-printed assembling tool and a MX sample holder. To validate the effectiveness of the proposed method, deoxyhemoglobin and methemoglobin samples were investigated using the BioMAX X-ray macromolecular crystallography beamline, the Balder X-ray absorption spectroscopy beamline and UV–Vis absorption spectroscopy. International Union of Crystallography 2023-11-01 /pmc/articles/PMC10619425/ /pubmed/37860963 http://dx.doi.org/10.1107/S205979832300880X Text en © Monika Bjelčić et al. 2023 https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited. |
spellingShingle | Research Papers Bjelčić, Monika Sigfridsson Clauss, Kajsa G. V. Aurelius, Oskar Milas, Mirko Nan, Jie Ursby, Thomas Anaerobic fixed-target serial crystallography using sandwiched silicon nitride membranes |
title | Anaerobic fixed-target serial crystallography using sandwiched silicon nitride membranes |
title_full | Anaerobic fixed-target serial crystallography using sandwiched silicon nitride membranes |
title_fullStr | Anaerobic fixed-target serial crystallography using sandwiched silicon nitride membranes |
title_full_unstemmed | Anaerobic fixed-target serial crystallography using sandwiched silicon nitride membranes |
title_short | Anaerobic fixed-target serial crystallography using sandwiched silicon nitride membranes |
title_sort | anaerobic fixed-target serial crystallography using sandwiched silicon nitride membranes |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10619425/ https://www.ncbi.nlm.nih.gov/pubmed/37860963 http://dx.doi.org/10.1107/S205979832300880X |
work_keys_str_mv | AT bjelcicmonika anaerobicfixedtargetserialcrystallographyusingsandwichedsiliconnitridemembranes AT sigfridssonclausskajsagv anaerobicfixedtargetserialcrystallographyusingsandwichedsiliconnitridemembranes AT aureliusoskar anaerobicfixedtargetserialcrystallographyusingsandwichedsiliconnitridemembranes AT milasmirko anaerobicfixedtargetserialcrystallographyusingsandwichedsiliconnitridemembranes AT nanjie anaerobicfixedtargetserialcrystallographyusingsandwichedsiliconnitridemembranes AT ursbythomas anaerobicfixedtargetserialcrystallographyusingsandwichedsiliconnitridemembranes |