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Serum proteomic profile of wild stump-tailed macaques (Macaca arctoides) infected with malaria parasites in Thailand

The number of patients infected with simian malaria is gradually increasing in many countries of Southeast Asia and South America. The most important risk factor for a zoonotic spillover event of malarial infection is mostly influenced by the interaction between humans, monkeys, and vectors. In this...

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Autores principales: Ruengket, Pakorn, Roytrakul, Sittiruk, Tongthainan, Daraka, Taruyanon, Kanokwan, Sangkharak, Bencharong, Limudomporn, Paviga, Pongsuchart, Mongkol, Udom, Chanya, Fungfuang, Wirasak
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10619813/
https://www.ncbi.nlm.nih.gov/pubmed/37910477
http://dx.doi.org/10.1371/journal.pone.0293579
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author Ruengket, Pakorn
Roytrakul, Sittiruk
Tongthainan, Daraka
Taruyanon, Kanokwan
Sangkharak, Bencharong
Limudomporn, Paviga
Pongsuchart, Mongkol
Udom, Chanya
Fungfuang, Wirasak
author_facet Ruengket, Pakorn
Roytrakul, Sittiruk
Tongthainan, Daraka
Taruyanon, Kanokwan
Sangkharak, Bencharong
Limudomporn, Paviga
Pongsuchart, Mongkol
Udom, Chanya
Fungfuang, Wirasak
author_sort Ruengket, Pakorn
collection PubMed
description The number of patients infected with simian malaria is gradually increasing in many countries of Southeast Asia and South America. The most important risk factor for a zoonotic spillover event of malarial infection is mostly influenced by the interaction between humans, monkeys, and vectors. In this study, we determine the protein expression profile of a wild stump-tailed macaque (Macaca arctoides) from a total of 32 blood samples collected from Prachuap Kiri Khan Province, Thailand. The malarial parasite was analyzed using nested polymerase chain reaction (PCR) assays by dividing the samples into three groups: non-infected, mono-infected, and multiple-infected. The identification and differential proteomic expression profiles were determined using liquid chromatography with tandem mass spectrometry (LC-MS/MS) and bioinformatics tools. A total of 9,532 proteins (total proteins) were identified with the filter-based selection methods analysis, and a subset of 440 proteins were found to be different between each group. Within these proteins, the GhostKOALA functional enrichment analysis indicated that 142 important proteins were associated with either of the organismal system (28.87%), genetic information processing (23.24%), environmental information processing (16.20%), metabolism (13.38%), cellular processes (11.97%), or causing human disease (6.34%). Additionally, using interaction network analysis, nine potential reporter proteins were identified. Here, we report the first study on the protein profiles differentially expressed in the serum of wild stump-tailed macaques between non, mono, and multiple malarial infected living in a natural transmission environment. Our findings demonstrate that differentially expressed proteins implicated in host defense through lipid metabolism, involved with TGF pathway were suppressed, while those with the apoptosis pathway, such as cytokines and proinflammation signals were increased. Including the parasite’s response via induced hemolysis and disruption of myeloid cells. A greater understanding of the fundamental processes involved in a malarial infection and host response can be crucial for developing diagnostic tools, medication development, and therapies to improve the health of those affected by the disease.
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spelling pubmed-106198132023-11-02 Serum proteomic profile of wild stump-tailed macaques (Macaca arctoides) infected with malaria parasites in Thailand Ruengket, Pakorn Roytrakul, Sittiruk Tongthainan, Daraka Taruyanon, Kanokwan Sangkharak, Bencharong Limudomporn, Paviga Pongsuchart, Mongkol Udom, Chanya Fungfuang, Wirasak PLoS One Research Article The number of patients infected with simian malaria is gradually increasing in many countries of Southeast Asia and South America. The most important risk factor for a zoonotic spillover event of malarial infection is mostly influenced by the interaction between humans, monkeys, and vectors. In this study, we determine the protein expression profile of a wild stump-tailed macaque (Macaca arctoides) from a total of 32 blood samples collected from Prachuap Kiri Khan Province, Thailand. The malarial parasite was analyzed using nested polymerase chain reaction (PCR) assays by dividing the samples into three groups: non-infected, mono-infected, and multiple-infected. The identification and differential proteomic expression profiles were determined using liquid chromatography with tandem mass spectrometry (LC-MS/MS) and bioinformatics tools. A total of 9,532 proteins (total proteins) were identified with the filter-based selection methods analysis, and a subset of 440 proteins were found to be different between each group. Within these proteins, the GhostKOALA functional enrichment analysis indicated that 142 important proteins were associated with either of the organismal system (28.87%), genetic information processing (23.24%), environmental information processing (16.20%), metabolism (13.38%), cellular processes (11.97%), or causing human disease (6.34%). Additionally, using interaction network analysis, nine potential reporter proteins were identified. Here, we report the first study on the protein profiles differentially expressed in the serum of wild stump-tailed macaques between non, mono, and multiple malarial infected living in a natural transmission environment. Our findings demonstrate that differentially expressed proteins implicated in host defense through lipid metabolism, involved with TGF pathway were suppressed, while those with the apoptosis pathway, such as cytokines and proinflammation signals were increased. Including the parasite’s response via induced hemolysis and disruption of myeloid cells. A greater understanding of the fundamental processes involved in a malarial infection and host response can be crucial for developing diagnostic tools, medication development, and therapies to improve the health of those affected by the disease. Public Library of Science 2023-11-01 /pmc/articles/PMC10619813/ /pubmed/37910477 http://dx.doi.org/10.1371/journal.pone.0293579 Text en © 2023 Ruengket et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Ruengket, Pakorn
Roytrakul, Sittiruk
Tongthainan, Daraka
Taruyanon, Kanokwan
Sangkharak, Bencharong
Limudomporn, Paviga
Pongsuchart, Mongkol
Udom, Chanya
Fungfuang, Wirasak
Serum proteomic profile of wild stump-tailed macaques (Macaca arctoides) infected with malaria parasites in Thailand
title Serum proteomic profile of wild stump-tailed macaques (Macaca arctoides) infected with malaria parasites in Thailand
title_full Serum proteomic profile of wild stump-tailed macaques (Macaca arctoides) infected with malaria parasites in Thailand
title_fullStr Serum proteomic profile of wild stump-tailed macaques (Macaca arctoides) infected with malaria parasites in Thailand
title_full_unstemmed Serum proteomic profile of wild stump-tailed macaques (Macaca arctoides) infected with malaria parasites in Thailand
title_short Serum proteomic profile of wild stump-tailed macaques (Macaca arctoides) infected with malaria parasites in Thailand
title_sort serum proteomic profile of wild stump-tailed macaques (macaca arctoides) infected with malaria parasites in thailand
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10619813/
https://www.ncbi.nlm.nih.gov/pubmed/37910477
http://dx.doi.org/10.1371/journal.pone.0293579
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