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PTGR1 is involved in cell proliferation in thoracic ossification of the ligamentum flavum

Thoracic ossification of the ligamentum flavum (TOLF) is a heterotopic ossification of spinal ligaments, leading to serious myelopathy. TOLF underlying mechanisms are not well understood. Our iTRAQ analysis have identified ten inflammatory factors related to TOLF, including l. We found that PTGR1 ex...

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Autores principales: Liu, Kuankuan, Shu, Li, Huang, Ann Yehong, Chang, Yanan, Chen, Zhongqiang, Zhang, Chi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10619815/
https://www.ncbi.nlm.nih.gov/pubmed/37910537
http://dx.doi.org/10.1371/journal.pone.0292821
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author Liu, Kuankuan
Shu, Li
Huang, Ann Yehong
Chang, Yanan
Chen, Zhongqiang
Zhang, Chi
author_facet Liu, Kuankuan
Shu, Li
Huang, Ann Yehong
Chang, Yanan
Chen, Zhongqiang
Zhang, Chi
author_sort Liu, Kuankuan
collection PubMed
description Thoracic ossification of the ligamentum flavum (TOLF) is a heterotopic ossification of spinal ligaments, leading to serious myelopathy. TOLF underlying mechanisms are not well understood. Our iTRAQ analysis have identified ten inflammatory factors related to TOLF, including l. We found that PTGR1 expressions increased in TOLF by RT-PCR and western blot in this study. Both cell proliferation and differentiation are important for the process of bone formation. In our previous study, we demonstrated that TOLF primary cells grew faster than control cells. It was reported that knockdown of PTGR1 inhibited cell proliferation. We hypothesize that PTGR1 may participate in cell proliferation in TOLF. To test this hypothesis, TOLF primary cells were treated for 24h with PTGR1. We observed that PTGR1 increased cell proliferation. The effect of PTGR1 on cell proliferation related genes was examined in TOLF primary cells. Our results showed that PTGR1 was able to activate expressions of c-Myc and CyclinD1. Moreover, blocking JNK pathway by selective JNK inhibitor SP600125 eliminated the positive effect of PTGR1 on c-Myc expression, indicating that PTGR1 activated the expression of c-Myc via JNK pathway. Our new findings suggest that PTGR1 is involved in cell proliferation of TOLF.
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spelling pubmed-106198152023-11-02 PTGR1 is involved in cell proliferation in thoracic ossification of the ligamentum flavum Liu, Kuankuan Shu, Li Huang, Ann Yehong Chang, Yanan Chen, Zhongqiang Zhang, Chi PLoS One Research Article Thoracic ossification of the ligamentum flavum (TOLF) is a heterotopic ossification of spinal ligaments, leading to serious myelopathy. TOLF underlying mechanisms are not well understood. Our iTRAQ analysis have identified ten inflammatory factors related to TOLF, including l. We found that PTGR1 expressions increased in TOLF by RT-PCR and western blot in this study. Both cell proliferation and differentiation are important for the process of bone formation. In our previous study, we demonstrated that TOLF primary cells grew faster than control cells. It was reported that knockdown of PTGR1 inhibited cell proliferation. We hypothesize that PTGR1 may participate in cell proliferation in TOLF. To test this hypothesis, TOLF primary cells were treated for 24h with PTGR1. We observed that PTGR1 increased cell proliferation. The effect of PTGR1 on cell proliferation related genes was examined in TOLF primary cells. Our results showed that PTGR1 was able to activate expressions of c-Myc and CyclinD1. Moreover, blocking JNK pathway by selective JNK inhibitor SP600125 eliminated the positive effect of PTGR1 on c-Myc expression, indicating that PTGR1 activated the expression of c-Myc via JNK pathway. Our new findings suggest that PTGR1 is involved in cell proliferation of TOLF. Public Library of Science 2023-11-01 /pmc/articles/PMC10619815/ /pubmed/37910537 http://dx.doi.org/10.1371/journal.pone.0292821 Text en © 2023 Liu et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Liu, Kuankuan
Shu, Li
Huang, Ann Yehong
Chang, Yanan
Chen, Zhongqiang
Zhang, Chi
PTGR1 is involved in cell proliferation in thoracic ossification of the ligamentum flavum
title PTGR1 is involved in cell proliferation in thoracic ossification of the ligamentum flavum
title_full PTGR1 is involved in cell proliferation in thoracic ossification of the ligamentum flavum
title_fullStr PTGR1 is involved in cell proliferation in thoracic ossification of the ligamentum flavum
title_full_unstemmed PTGR1 is involved in cell proliferation in thoracic ossification of the ligamentum flavum
title_short PTGR1 is involved in cell proliferation in thoracic ossification of the ligamentum flavum
title_sort ptgr1 is involved in cell proliferation in thoracic ossification of the ligamentum flavum
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10619815/
https://www.ncbi.nlm.nih.gov/pubmed/37910537
http://dx.doi.org/10.1371/journal.pone.0292821
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