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Melatonin improves the vitrification of sheep morulae by modulating transcriptome

Embryo vitrification technology is widely used in livestock production, but freezing injury has been a key factor hindering the efficiency of embryo production. There is an urgent need to further analyze the molecular mechanism of embryo damage by the vitrification process. In the study, morulae wer...

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Autores principales: Ji, Pengyun, Liu, Yunjie, Yan, Laiqing, Jia, Yanquan, Zhao, Mengmeng, Lv, Dongying, Yao, Yujun, Ma, Wenkui, Yin, Depeng, Liu, Fenze, Gao, Shuai, Wusiman, Abulizi, Yang, Kailun, Zhang, Lu, Liu, Guoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10619913/
https://www.ncbi.nlm.nih.gov/pubmed/37920328
http://dx.doi.org/10.3389/fvets.2023.1212047
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author Ji, Pengyun
Liu, Yunjie
Yan, Laiqing
Jia, Yanquan
Zhao, Mengmeng
Lv, Dongying
Yao, Yujun
Ma, Wenkui
Yin, Depeng
Liu, Fenze
Gao, Shuai
Wusiman, Abulizi
Yang, Kailun
Zhang, Lu
Liu, Guoshi
author_facet Ji, Pengyun
Liu, Yunjie
Yan, Laiqing
Jia, Yanquan
Zhao, Mengmeng
Lv, Dongying
Yao, Yujun
Ma, Wenkui
Yin, Depeng
Liu, Fenze
Gao, Shuai
Wusiman, Abulizi
Yang, Kailun
Zhang, Lu
Liu, Guoshi
author_sort Ji, Pengyun
collection PubMed
description Embryo vitrification technology is widely used in livestock production, but freezing injury has been a key factor hindering the efficiency of embryo production. There is an urgent need to further analyze the molecular mechanism of embryo damage by the vitrification process. In the study, morulae were collected from Hu sheep uterine horns after superovulation and sperm transfusion. Morulae were Cryotop vitrified and warmed. Nine morulae were in the vitrified control group (frozen), and seven morulae were vitrified and warmed with 10(−5) M melatonin (melatonin). Eleven non-frozen morulae were used as controls (fresh). After warming, each embryo was sequenced separately for library construction and gene expression analysis. p < 0.05 was used to differentiate differentially expressed genes (DEG). The results showed that differentiated differentially expressed genes (DEG) in vitrified morulae were mainly enriched in protein kinase activity, adhesion processes, calcium signaling pathways and Wnt, PI3K/AKT, Ras, ErbB, and MAPK signaling pathways compared to controls. Importantly, melatonin treatment upregulated the expression of key pathways that increase the resistance of morulae against vitrification induced damage. These pathways include kinase activity pathway, ErbB, and PI3K/Akt signaling pathway. It is worth mentioning that melatonin upregulates the expression of XPA, which is a key transcription factor for DNA repair. In conclusion, vitrification affected the transcriptome of in vivo-derived Hu sheep morulae, and melatonin had a protective effect on the vitrification process. For the first time, the transcriptome profiles caused by vitrification and melatonin in sheep morulae were analyzed in single embryo level. These data obtained from the single embryo level provide an important molecular mechanism for further optimizing the cryopreservation of embryos or other cells.
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spelling pubmed-106199132023-11-02 Melatonin improves the vitrification of sheep morulae by modulating transcriptome Ji, Pengyun Liu, Yunjie Yan, Laiqing Jia, Yanquan Zhao, Mengmeng Lv, Dongying Yao, Yujun Ma, Wenkui Yin, Depeng Liu, Fenze Gao, Shuai Wusiman, Abulizi Yang, Kailun Zhang, Lu Liu, Guoshi Front Vet Sci Veterinary Science Embryo vitrification technology is widely used in livestock production, but freezing injury has been a key factor hindering the efficiency of embryo production. There is an urgent need to further analyze the molecular mechanism of embryo damage by the vitrification process. In the study, morulae were collected from Hu sheep uterine horns after superovulation and sperm transfusion. Morulae were Cryotop vitrified and warmed. Nine morulae were in the vitrified control group (frozen), and seven morulae were vitrified and warmed with 10(−5) M melatonin (melatonin). Eleven non-frozen morulae were used as controls (fresh). After warming, each embryo was sequenced separately for library construction and gene expression analysis. p < 0.05 was used to differentiate differentially expressed genes (DEG). The results showed that differentiated differentially expressed genes (DEG) in vitrified morulae were mainly enriched in protein kinase activity, adhesion processes, calcium signaling pathways and Wnt, PI3K/AKT, Ras, ErbB, and MAPK signaling pathways compared to controls. Importantly, melatonin treatment upregulated the expression of key pathways that increase the resistance of morulae against vitrification induced damage. These pathways include kinase activity pathway, ErbB, and PI3K/Akt signaling pathway. It is worth mentioning that melatonin upregulates the expression of XPA, which is a key transcription factor for DNA repair. In conclusion, vitrification affected the transcriptome of in vivo-derived Hu sheep morulae, and melatonin had a protective effect on the vitrification process. For the first time, the transcriptome profiles caused by vitrification and melatonin in sheep morulae were analyzed in single embryo level. These data obtained from the single embryo level provide an important molecular mechanism for further optimizing the cryopreservation of embryos or other cells. Frontiers Media S.A. 2023-10-18 /pmc/articles/PMC10619913/ /pubmed/37920328 http://dx.doi.org/10.3389/fvets.2023.1212047 Text en Copyright © 2023 Ji, Liu, Yan, Jia, Zhao, Lv, Yao, Ma, Yin, Liu, Gao, Wusiman, Yang, Zhang and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Ji, Pengyun
Liu, Yunjie
Yan, Laiqing
Jia, Yanquan
Zhao, Mengmeng
Lv, Dongying
Yao, Yujun
Ma, Wenkui
Yin, Depeng
Liu, Fenze
Gao, Shuai
Wusiman, Abulizi
Yang, Kailun
Zhang, Lu
Liu, Guoshi
Melatonin improves the vitrification of sheep morulae by modulating transcriptome
title Melatonin improves the vitrification of sheep morulae by modulating transcriptome
title_full Melatonin improves the vitrification of sheep morulae by modulating transcriptome
title_fullStr Melatonin improves the vitrification of sheep morulae by modulating transcriptome
title_full_unstemmed Melatonin improves the vitrification of sheep morulae by modulating transcriptome
title_short Melatonin improves the vitrification of sheep morulae by modulating transcriptome
title_sort melatonin improves the vitrification of sheep morulae by modulating transcriptome
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10619913/
https://www.ncbi.nlm.nih.gov/pubmed/37920328
http://dx.doi.org/10.3389/fvets.2023.1212047
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