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Interactions between gold nanoparticles with different morphologies and human serum albumin

Introduction: Three different shapes of gold nanoparticles were synthesized in this experiment. At the same time, studies compared their effects with human serum albumin (HSA). Methods: Gold nanoparticles (AuNPs) with three different morphologies, such as, nanospheres (AuNSs), nanorods (AuNRs), and...

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Autores principales: Dai, Jiahui, Chen, Chao, Yin, Man, Li, Huixing, Li, Wenbo, Zhang, Zhaowei, Wang, Qian, Du, Zhongyu, Xu, Xiangyu, Wang, Yunfei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10620604/
https://www.ncbi.nlm.nih.gov/pubmed/37927561
http://dx.doi.org/10.3389/fchem.2023.1273388
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author Dai, Jiahui
Chen, Chao
Yin, Man
Li, Huixing
Li, Wenbo
Zhang, Zhaowei
Wang, Qian
Du, Zhongyu
Xu, Xiangyu
Wang, Yunfei
author_facet Dai, Jiahui
Chen, Chao
Yin, Man
Li, Huixing
Li, Wenbo
Zhang, Zhaowei
Wang, Qian
Du, Zhongyu
Xu, Xiangyu
Wang, Yunfei
author_sort Dai, Jiahui
collection PubMed
description Introduction: Three different shapes of gold nanoparticles were synthesized in this experiment. At the same time, studies compared their effects with human serum albumin (HSA). Methods: Gold nanoparticles (AuNPs) with three different morphologies, such as, nanospheres (AuNSs), nanorods (AuNRs), and nanoflowers (AuNFs) were synthesized via a seeding method and their characteristic absorption peaks were detected using ultraviolet-visible (UV-vis) absorption spectroscopy, Telectron microscopy (TEM), Dynamic Light Scattering (DLS) and Zeta potential measurements, circular dichroism (CD), and Fourier transform infrared spectroscopy (FTIR) to study the interactions between them and HSA. By comparing the thermodynamic parameters and quenching mechanism of the three materials, similarities and differences were determined in their interactions with HSA. Results: The results showed that with an increase in the concentration of the AuNPs with the three different morphologies, the UV-vis absorption peak intensity of the mixed solution increased, but its fluorescence intensity was quenched. This indicates that the three types of AuNPs interact with HSA, and that the interactions between them represent a static quenching process, which is consistent with the conclusions derived from three-dimensional fluorescence experiments. Through variable-temperature fluorescence experiments, the binding constants, number of binding sites, and thermodynamic parameters of the interactions between the three types of AuNPs and HSA were determined. The Gibbs free energy changes were <0, indicating that the reactions of the three types of AuNPs with HSA are spontaneous, resulting in associated matter. Binding constant measurements indicated that the strongest binding took place between the AuNFs and HSA. In addition, the results of fluorescence, CD spectroscopy, and FTIR showed that three different shapes of AuNPs can induce conformational changes in HSA and reduce the α-helix content. Among them, AuNFs have the smallest ability to induce conformational changes. Discussion: According to studies, AuNFs interact more favorably with HSA. This can be used as a reference for the administration of drugs containing AuNPs.
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spelling pubmed-106206042023-11-03 Interactions between gold nanoparticles with different morphologies and human serum albumin Dai, Jiahui Chen, Chao Yin, Man Li, Huixing Li, Wenbo Zhang, Zhaowei Wang, Qian Du, Zhongyu Xu, Xiangyu Wang, Yunfei Front Chem Chemistry Introduction: Three different shapes of gold nanoparticles were synthesized in this experiment. At the same time, studies compared their effects with human serum albumin (HSA). Methods: Gold nanoparticles (AuNPs) with three different morphologies, such as, nanospheres (AuNSs), nanorods (AuNRs), and nanoflowers (AuNFs) were synthesized via a seeding method and their characteristic absorption peaks were detected using ultraviolet-visible (UV-vis) absorption spectroscopy, Telectron microscopy (TEM), Dynamic Light Scattering (DLS) and Zeta potential measurements, circular dichroism (CD), and Fourier transform infrared spectroscopy (FTIR) to study the interactions between them and HSA. By comparing the thermodynamic parameters and quenching mechanism of the three materials, similarities and differences were determined in their interactions with HSA. Results: The results showed that with an increase in the concentration of the AuNPs with the three different morphologies, the UV-vis absorption peak intensity of the mixed solution increased, but its fluorescence intensity was quenched. This indicates that the three types of AuNPs interact with HSA, and that the interactions between them represent a static quenching process, which is consistent with the conclusions derived from three-dimensional fluorescence experiments. Through variable-temperature fluorescence experiments, the binding constants, number of binding sites, and thermodynamic parameters of the interactions between the three types of AuNPs and HSA were determined. The Gibbs free energy changes were <0, indicating that the reactions of the three types of AuNPs with HSA are spontaneous, resulting in associated matter. Binding constant measurements indicated that the strongest binding took place between the AuNFs and HSA. In addition, the results of fluorescence, CD spectroscopy, and FTIR showed that three different shapes of AuNPs can induce conformational changes in HSA and reduce the α-helix content. Among them, AuNFs have the smallest ability to induce conformational changes. Discussion: According to studies, AuNFs interact more favorably with HSA. This can be used as a reference for the administration of drugs containing AuNPs. Frontiers Media S.A. 2023-10-19 /pmc/articles/PMC10620604/ /pubmed/37927561 http://dx.doi.org/10.3389/fchem.2023.1273388 Text en Copyright © 2023 Dai, Chen, Yin, Li, Li, Zhang, Wang, Du, Xu and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Chemistry
Dai, Jiahui
Chen, Chao
Yin, Man
Li, Huixing
Li, Wenbo
Zhang, Zhaowei
Wang, Qian
Du, Zhongyu
Xu, Xiangyu
Wang, Yunfei
Interactions between gold nanoparticles with different morphologies and human serum albumin
title Interactions between gold nanoparticles with different morphologies and human serum albumin
title_full Interactions between gold nanoparticles with different morphologies and human serum albumin
title_fullStr Interactions between gold nanoparticles with different morphologies and human serum albumin
title_full_unstemmed Interactions between gold nanoparticles with different morphologies and human serum albumin
title_short Interactions between gold nanoparticles with different morphologies and human serum albumin
title_sort interactions between gold nanoparticles with different morphologies and human serum albumin
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10620604/
https://www.ncbi.nlm.nih.gov/pubmed/37927561
http://dx.doi.org/10.3389/fchem.2023.1273388
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