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Antimycobacterial Precatorin A Flavonoid Displays Antibiofilm Activity against Mycobacterium bovis BCG

[Image: see text] The aim of this study was to evaluate the potential antibiofilm activity of Rhynchosia precatoria (R. precatoria) compounds over Mycobacterium bovis BCG (M. bovis BCG) as a model for Mycobacterium tuberculosis (Mtb). We evaluated the antibiofilm activity as the ability to both inhi...

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Autores principales: Niño-Padilla, Esmeralda Ivonne, Espitia, Clara, Velazquez, Carlos, Alday, Efrain, Silva-Campa, Erika, Burgara-Estrella, Alexel, Enciso-Moreno, José Antonio, Valenzuela, Olivia, Astiazarán-García, Humberto, Garibay-Escobar, Adriana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10621015/
https://www.ncbi.nlm.nih.gov/pubmed/37929145
http://dx.doi.org/10.1021/acsomega.3c05703
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author Niño-Padilla, Esmeralda Ivonne
Espitia, Clara
Velazquez, Carlos
Alday, Efrain
Silva-Campa, Erika
Burgara-Estrella, Alexel
Enciso-Moreno, José Antonio
Valenzuela, Olivia
Astiazarán-García, Humberto
Garibay-Escobar, Adriana
author_facet Niño-Padilla, Esmeralda Ivonne
Espitia, Clara
Velazquez, Carlos
Alday, Efrain
Silva-Campa, Erika
Burgara-Estrella, Alexel
Enciso-Moreno, José Antonio
Valenzuela, Olivia
Astiazarán-García, Humberto
Garibay-Escobar, Adriana
author_sort Niño-Padilla, Esmeralda Ivonne
collection PubMed
description [Image: see text] The aim of this study was to evaluate the potential antibiofilm activity of Rhynchosia precatoria (R. precatoria) compounds over Mycobacterium bovis BCG (M. bovis BCG) as a model for Mycobacterium tuberculosis (Mtb). We evaluated the antibiofilm activity as the ability to both inhibit biofilm formation and disrupt preformed biofilms (bactericidal) of R. precatoria compounds, which have been previously described as being antimycobacterials against Mtb. M. bovis BCG developed air–liquid interface biofilms with surface attachment ability and drug tolerance. Of the R. precatoria extracts and compounds that were tested, precatorin A (PreA) displayed the best biofilm inhibitory activity, as evaluated by biofilm biomass quantification, viable cell count, and confocal and atomic force microscopy procedures. Furthermore, its combination with isoniazid at subinhibitory concentrations inhibited M. bovis BCG biofilm formation. Nonetheless, neither PreA nor the extract showed bactericidal effects. PreA is the R. precatoria compound responsible for biofilm inhibitory activity against M. bovis BCG.
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spelling pubmed-106210152023-11-03 Antimycobacterial Precatorin A Flavonoid Displays Antibiofilm Activity against Mycobacterium bovis BCG Niño-Padilla, Esmeralda Ivonne Espitia, Clara Velazquez, Carlos Alday, Efrain Silva-Campa, Erika Burgara-Estrella, Alexel Enciso-Moreno, José Antonio Valenzuela, Olivia Astiazarán-García, Humberto Garibay-Escobar, Adriana ACS Omega [Image: see text] The aim of this study was to evaluate the potential antibiofilm activity of Rhynchosia precatoria (R. precatoria) compounds over Mycobacterium bovis BCG (M. bovis BCG) as a model for Mycobacterium tuberculosis (Mtb). We evaluated the antibiofilm activity as the ability to both inhibit biofilm formation and disrupt preformed biofilms (bactericidal) of R. precatoria compounds, which have been previously described as being antimycobacterials against Mtb. M. bovis BCG developed air–liquid interface biofilms with surface attachment ability and drug tolerance. Of the R. precatoria extracts and compounds that were tested, precatorin A (PreA) displayed the best biofilm inhibitory activity, as evaluated by biofilm biomass quantification, viable cell count, and confocal and atomic force microscopy procedures. Furthermore, its combination with isoniazid at subinhibitory concentrations inhibited M. bovis BCG biofilm formation. Nonetheless, neither PreA nor the extract showed bactericidal effects. PreA is the R. precatoria compound responsible for biofilm inhibitory activity against M. bovis BCG. American Chemical Society 2023-10-20 /pmc/articles/PMC10621015/ /pubmed/37929145 http://dx.doi.org/10.1021/acsomega.3c05703 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Niño-Padilla, Esmeralda Ivonne
Espitia, Clara
Velazquez, Carlos
Alday, Efrain
Silva-Campa, Erika
Burgara-Estrella, Alexel
Enciso-Moreno, José Antonio
Valenzuela, Olivia
Astiazarán-García, Humberto
Garibay-Escobar, Adriana
Antimycobacterial Precatorin A Flavonoid Displays Antibiofilm Activity against Mycobacterium bovis BCG
title Antimycobacterial Precatorin A Flavonoid Displays Antibiofilm Activity against Mycobacterium bovis BCG
title_full Antimycobacterial Precatorin A Flavonoid Displays Antibiofilm Activity against Mycobacterium bovis BCG
title_fullStr Antimycobacterial Precatorin A Flavonoid Displays Antibiofilm Activity against Mycobacterium bovis BCG
title_full_unstemmed Antimycobacterial Precatorin A Flavonoid Displays Antibiofilm Activity against Mycobacterium bovis BCG
title_short Antimycobacterial Precatorin A Flavonoid Displays Antibiofilm Activity against Mycobacterium bovis BCG
title_sort antimycobacterial precatorin a flavonoid displays antibiofilm activity against mycobacterium bovis bcg
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10621015/
https://www.ncbi.nlm.nih.gov/pubmed/37929145
http://dx.doi.org/10.1021/acsomega.3c05703
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