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Molecular detection of Babesia spp. in dogs in Germany (2007–2020) and identification of potential risk factors for infection

BACKGROUND: In Europe, canine babesiosis is most frequently caused by Babesia canis and Babesia vogeli, and occasionally by Babesia gibsoni.. In Germany, B. canis is recognized as endemic. The aims of this study were to assess how often Babesia spp. infections were diagnosed in a commercial laborato...

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Autores principales: Schäfer, Ingo, Helm, Christina Sabine, von Samson-Himmelstjerna, Georg, Krücken, Jürgen, Kottmann, Tanja, Holtdirk, Annette, Kohn, Barbara, Hendrickx, Guy, Marsboom, Cedric, Müller, Elisabeth
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10621212/
https://www.ncbi.nlm.nih.gov/pubmed/37919757
http://dx.doi.org/10.1186/s13071-023-06005-7
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author Schäfer, Ingo
Helm, Christina Sabine
von Samson-Himmelstjerna, Georg
Krücken, Jürgen
Kottmann, Tanja
Holtdirk, Annette
Kohn, Barbara
Hendrickx, Guy
Marsboom, Cedric
Müller, Elisabeth
author_facet Schäfer, Ingo
Helm, Christina Sabine
von Samson-Himmelstjerna, Georg
Krücken, Jürgen
Kottmann, Tanja
Holtdirk, Annette
Kohn, Barbara
Hendrickx, Guy
Marsboom, Cedric
Müller, Elisabeth
author_sort Schäfer, Ingo
collection PubMed
description BACKGROUND: In Europe, canine babesiosis is most frequently caused by Babesia canis and Babesia vogeli, and occasionally by Babesia gibsoni.. In Germany, B. canis is recognized as endemic. The aims of this study were to assess how often Babesia spp. infections were diagnosed in a commercial laboratory in samples from dogs from Germany, and to evaluate potential risk factors for infection. METHODS: The database of the LABOKLIN laboratory was screened for Babesia spp.-positive polymerase chain reaction (PCR) tests for dogs for the period January 2007–December 2020. Sequencing was performed for positive tests from 2018 and 2019. Binary logistic regression analysis was performed to determine the effects of sex, season, and year of testing. Questionnaires were sent to the submitting veterinarians to obtain information on travel abroad, tick infestation, and ectoparasite prophylaxis of the respective dogs. Fisher’s exact test was used to calculate statistical significance and P < 0.05 was considered statistically significant. RESULTS: In total, 659 out of 20,914 dogs (3.2%) tested positive for Babesia spp. by PCR. Of 172 sequenced samples, B. canis was identified in 156, B. vogeli in nine, B. gibsoni in five, and B. vulpes in two. Season had a statistically significant impact on test results when summer/winter (1.6% tested positive) was compared to spring/autumn (4.7%), with peaks in April (5.2%) and October (7.4%) [P < 0.001, odds ratio (OR) = 3.16]. Sex (male 3.5%, female 2.8%; P = 0.012, OR = 1.49) and age (< 7 years old 4.0%, ≥ 7 years old 2.3%; P < 0.001, OR = 1.76) of the tested dogs also had a statistically significant effect. A statistically significant impact was demonstrated for observed tick attachment (P < 0.001, OR = 7.62) and lack of ectoparasite prophylaxis (P = 0.001, OR = 3.03). The frequency of positive Babesia spp. tests did not significantly differ between the 659 dogs that had never left Germany and the 1506 dogs with known stays abroad (P = 0.088). CONCLUSIONS: The possibility of canine infection with B. canis needs to be especially taken into consideration in spring and autumn in Germany as the activity of the tick Dermacentor reticulatus, a potential vector for canine babesiosis, is highest in these seasons. Travel and importation of dogs are considered major factors associated with canine babesiosis in Germany. However, autochthonous Babesia spp. infections also occur in a considerable number of dogs in Germany. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13071-023-06005-7.
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spelling pubmed-106212122023-11-03 Molecular detection of Babesia spp. in dogs in Germany (2007–2020) and identification of potential risk factors for infection Schäfer, Ingo Helm, Christina Sabine von Samson-Himmelstjerna, Georg Krücken, Jürgen Kottmann, Tanja Holtdirk, Annette Kohn, Barbara Hendrickx, Guy Marsboom, Cedric Müller, Elisabeth Parasit Vectors Research BACKGROUND: In Europe, canine babesiosis is most frequently caused by Babesia canis and Babesia vogeli, and occasionally by Babesia gibsoni.. In Germany, B. canis is recognized as endemic. The aims of this study were to assess how often Babesia spp. infections were diagnosed in a commercial laboratory in samples from dogs from Germany, and to evaluate potential risk factors for infection. METHODS: The database of the LABOKLIN laboratory was screened for Babesia spp.-positive polymerase chain reaction (PCR) tests for dogs for the period January 2007–December 2020. Sequencing was performed for positive tests from 2018 and 2019. Binary logistic regression analysis was performed to determine the effects of sex, season, and year of testing. Questionnaires were sent to the submitting veterinarians to obtain information on travel abroad, tick infestation, and ectoparasite prophylaxis of the respective dogs. Fisher’s exact test was used to calculate statistical significance and P < 0.05 was considered statistically significant. RESULTS: In total, 659 out of 20,914 dogs (3.2%) tested positive for Babesia spp. by PCR. Of 172 sequenced samples, B. canis was identified in 156, B. vogeli in nine, B. gibsoni in five, and B. vulpes in two. Season had a statistically significant impact on test results when summer/winter (1.6% tested positive) was compared to spring/autumn (4.7%), with peaks in April (5.2%) and October (7.4%) [P < 0.001, odds ratio (OR) = 3.16]. Sex (male 3.5%, female 2.8%; P = 0.012, OR = 1.49) and age (< 7 years old 4.0%, ≥ 7 years old 2.3%; P < 0.001, OR = 1.76) of the tested dogs also had a statistically significant effect. A statistically significant impact was demonstrated for observed tick attachment (P < 0.001, OR = 7.62) and lack of ectoparasite prophylaxis (P = 0.001, OR = 3.03). The frequency of positive Babesia spp. tests did not significantly differ between the 659 dogs that had never left Germany and the 1506 dogs with known stays abroad (P = 0.088). CONCLUSIONS: The possibility of canine infection with B. canis needs to be especially taken into consideration in spring and autumn in Germany as the activity of the tick Dermacentor reticulatus, a potential vector for canine babesiosis, is highest in these seasons. Travel and importation of dogs are considered major factors associated with canine babesiosis in Germany. However, autochthonous Babesia spp. infections also occur in a considerable number of dogs in Germany. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13071-023-06005-7. BioMed Central 2023-11-02 /pmc/articles/PMC10621212/ /pubmed/37919757 http://dx.doi.org/10.1186/s13071-023-06005-7 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Schäfer, Ingo
Helm, Christina Sabine
von Samson-Himmelstjerna, Georg
Krücken, Jürgen
Kottmann, Tanja
Holtdirk, Annette
Kohn, Barbara
Hendrickx, Guy
Marsboom, Cedric
Müller, Elisabeth
Molecular detection of Babesia spp. in dogs in Germany (2007–2020) and identification of potential risk factors for infection
title Molecular detection of Babesia spp. in dogs in Germany (2007–2020) and identification of potential risk factors for infection
title_full Molecular detection of Babesia spp. in dogs in Germany (2007–2020) and identification of potential risk factors for infection
title_fullStr Molecular detection of Babesia spp. in dogs in Germany (2007–2020) and identification of potential risk factors for infection
title_full_unstemmed Molecular detection of Babesia spp. in dogs in Germany (2007–2020) and identification of potential risk factors for infection
title_short Molecular detection of Babesia spp. in dogs in Germany (2007–2020) and identification of potential risk factors for infection
title_sort molecular detection of babesia spp. in dogs in germany (2007–2020) and identification of potential risk factors for infection
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10621212/
https://www.ncbi.nlm.nih.gov/pubmed/37919757
http://dx.doi.org/10.1186/s13071-023-06005-7
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