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Tim-3 Is Differentially Expressed during Cell Activation and Interacts with the LSP-1 Protein in Human Macrophages
T-cell Immunoglobulin and Mucin Domain 3 (TIM-3) is an immune checkpoint receptor known to regulate T-cell activation and has been targeted for immunotherapy in cancer and other diseases. However, its expression and function in other cell types, such as macrophages, are poorly understood. This study...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10622183/ https://www.ncbi.nlm.nih.gov/pubmed/37928435 http://dx.doi.org/10.1155/2023/3577334 |
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author | Ocaña-Guzman, Ranferi Ramon-Luing, Lucero A. Vazquez-Bolaños, Luis A. Rodríguez-Alvarado, Michelle Bulhusen-Rodriguez, Fausi Torres-Hatem, Alonso Gonzalez-Torres, Karen de Alba-Alvarado, Mariana Citlalli Sada-Ovalle, Isabel |
author_facet | Ocaña-Guzman, Ranferi Ramon-Luing, Lucero A. Vazquez-Bolaños, Luis A. Rodríguez-Alvarado, Michelle Bulhusen-Rodriguez, Fausi Torres-Hatem, Alonso Gonzalez-Torres, Karen de Alba-Alvarado, Mariana Citlalli Sada-Ovalle, Isabel |
author_sort | Ocaña-Guzman, Ranferi |
collection | PubMed |
description | T-cell Immunoglobulin and Mucin Domain 3 (TIM-3) is an immune checkpoint receptor known to regulate T-cell activation and has been targeted for immunotherapy in cancer and other diseases. However, its expression and function in other cell types, such as macrophages, are poorly understood. This study investigated TIM-3 expression in human macrophages polarized to M1 (stimulated with IFN-γ and LPS) and M2 (stimulated with IL-4 and IL-13) phenotypes using an in vitro model. Our results show that M1 macrophages have a lower frequency of TIM-3+ cells compared to M2 macrophages at 48 and 72 hr poststimulation. Additionally, we observed differential levels of soluble ADAM 10, an enzyme responsible for TIM-3 release, in the supernatants of M1 and M2 macrophages at 72 hr. We also found that the TIM-3 intracellular tail might associate with lymphocyte-specific protein 1 (LSP-1), a protein implicated in cell motility and podosome formation. These findings enhance our understanding of TIM-3 function in myeloid cells such as macrophages and may inform the development of immunotherapies with reduced immune-related adverse effects. |
format | Online Article Text |
id | pubmed-10622183 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-106221832023-11-03 Tim-3 Is Differentially Expressed during Cell Activation and Interacts with the LSP-1 Protein in Human Macrophages Ocaña-Guzman, Ranferi Ramon-Luing, Lucero A. Vazquez-Bolaños, Luis A. Rodríguez-Alvarado, Michelle Bulhusen-Rodriguez, Fausi Torres-Hatem, Alonso Gonzalez-Torres, Karen de Alba-Alvarado, Mariana Citlalli Sada-Ovalle, Isabel J Immunol Res Research Article T-cell Immunoglobulin and Mucin Domain 3 (TIM-3) is an immune checkpoint receptor known to regulate T-cell activation and has been targeted for immunotherapy in cancer and other diseases. However, its expression and function in other cell types, such as macrophages, are poorly understood. This study investigated TIM-3 expression in human macrophages polarized to M1 (stimulated with IFN-γ and LPS) and M2 (stimulated with IL-4 and IL-13) phenotypes using an in vitro model. Our results show that M1 macrophages have a lower frequency of TIM-3+ cells compared to M2 macrophages at 48 and 72 hr poststimulation. Additionally, we observed differential levels of soluble ADAM 10, an enzyme responsible for TIM-3 release, in the supernatants of M1 and M2 macrophages at 72 hr. We also found that the TIM-3 intracellular tail might associate with lymphocyte-specific protein 1 (LSP-1), a protein implicated in cell motility and podosome formation. These findings enhance our understanding of TIM-3 function in myeloid cells such as macrophages and may inform the development of immunotherapies with reduced immune-related adverse effects. Hindawi 2023-10-26 /pmc/articles/PMC10622183/ /pubmed/37928435 http://dx.doi.org/10.1155/2023/3577334 Text en Copyright © 2023 Ranferi Ocaña-Guzman et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Ocaña-Guzman, Ranferi Ramon-Luing, Lucero A. Vazquez-Bolaños, Luis A. Rodríguez-Alvarado, Michelle Bulhusen-Rodriguez, Fausi Torres-Hatem, Alonso Gonzalez-Torres, Karen de Alba-Alvarado, Mariana Citlalli Sada-Ovalle, Isabel Tim-3 Is Differentially Expressed during Cell Activation and Interacts with the LSP-1 Protein in Human Macrophages |
title | Tim-3 Is Differentially Expressed during Cell Activation and Interacts with the LSP-1 Protein in Human Macrophages |
title_full | Tim-3 Is Differentially Expressed during Cell Activation and Interacts with the LSP-1 Protein in Human Macrophages |
title_fullStr | Tim-3 Is Differentially Expressed during Cell Activation and Interacts with the LSP-1 Protein in Human Macrophages |
title_full_unstemmed | Tim-3 Is Differentially Expressed during Cell Activation and Interacts with the LSP-1 Protein in Human Macrophages |
title_short | Tim-3 Is Differentially Expressed during Cell Activation and Interacts with the LSP-1 Protein in Human Macrophages |
title_sort | tim-3 is differentially expressed during cell activation and interacts with the lsp-1 protein in human macrophages |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10622183/ https://www.ncbi.nlm.nih.gov/pubmed/37928435 http://dx.doi.org/10.1155/2023/3577334 |
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