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Glycosyl hydrolase from Pseudomonas fluorescens inhibits the biofilm formation of Pseudomonads

Biofilms are complex microbial communities embedded in extracellular matrix. Pathogens within the biofilm become more resistant to the antibiotics than planktonic counterparts. Novel strategies are required to encounter biofilms. Exopolysaccharides are one of the major components of biofilm matrix a...

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Autores principales: Wang, Di, Naqvi, Syed Tatheer Alam, Lei, Fanglin, Zhang, Zhenyu, Yu, Haiying, Ma, Luyan Z.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10622837/
https://www.ncbi.nlm.nih.gov/pubmed/37928620
http://dx.doi.org/10.1016/j.bioflm.2023.100155
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author Wang, Di
Naqvi, Syed Tatheer Alam
Lei, Fanglin
Zhang, Zhenyu
Yu, Haiying
Ma, Luyan Z.
author_facet Wang, Di
Naqvi, Syed Tatheer Alam
Lei, Fanglin
Zhang, Zhenyu
Yu, Haiying
Ma, Luyan Z.
author_sort Wang, Di
collection PubMed
description Biofilms are complex microbial communities embedded in extracellular matrix. Pathogens within the biofilm become more resistant to the antibiotics than planktonic counterparts. Novel strategies are required to encounter biofilms. Exopolysaccharides are one of the major components of biofilm matrix and play a vital role in biofilm architecture. In previous studies, a glycosyl hydrolase, PslG(PA), from Pseudomonas aeruginosa was found to be able to inhibit biofilm formation by disintegrating exopolysaccharide in biofilms. Here, we investigate the potential spectrum of PslG homologous protein with anti-biofilm activity. One glycosyl hydrolase from Pseudomonas fluorescens, PslG(PF), exhibits anti-biofilm activities and the key catalytic residues of PslG(PF) are conserved with those of PslG(PA). PslG(PF) at concentrations as low as 50 nM efficiently inhibits the biofilm formation of P. aeruginosa and disassemble its preformed biofilm. Furthermore, PslG(PF) exhibits anti-biofilm activity on a series of Pseudomonads, including P. fluorescens, Pseudomonas stutzeri and Pseudomonas syringae pv. phaseolicola. PslG(PF) stays active under various temperatures. Our findings suggest that P. fluorescens glycosyl hydrolase PslG(PF) has potential to be a broad spectrum inhibitor on biofilm formation of a wide range of Pseudomonads.
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spelling pubmed-106228372023-11-04 Glycosyl hydrolase from Pseudomonas fluorescens inhibits the biofilm formation of Pseudomonads Wang, Di Naqvi, Syed Tatheer Alam Lei, Fanglin Zhang, Zhenyu Yu, Haiying Ma, Luyan Z. Biofilm Article Biofilms are complex microbial communities embedded in extracellular matrix. Pathogens within the biofilm become more resistant to the antibiotics than planktonic counterparts. Novel strategies are required to encounter biofilms. Exopolysaccharides are one of the major components of biofilm matrix and play a vital role in biofilm architecture. In previous studies, a glycosyl hydrolase, PslG(PA), from Pseudomonas aeruginosa was found to be able to inhibit biofilm formation by disintegrating exopolysaccharide in biofilms. Here, we investigate the potential spectrum of PslG homologous protein with anti-biofilm activity. One glycosyl hydrolase from Pseudomonas fluorescens, PslG(PF), exhibits anti-biofilm activities and the key catalytic residues of PslG(PF) are conserved with those of PslG(PA). PslG(PF) at concentrations as low as 50 nM efficiently inhibits the biofilm formation of P. aeruginosa and disassemble its preformed biofilm. Furthermore, PslG(PF) exhibits anti-biofilm activity on a series of Pseudomonads, including P. fluorescens, Pseudomonas stutzeri and Pseudomonas syringae pv. phaseolicola. PslG(PF) stays active under various temperatures. Our findings suggest that P. fluorescens glycosyl hydrolase PslG(PF) has potential to be a broad spectrum inhibitor on biofilm formation of a wide range of Pseudomonads. Elsevier 2023-09-16 /pmc/articles/PMC10622837/ /pubmed/37928620 http://dx.doi.org/10.1016/j.bioflm.2023.100155 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Wang, Di
Naqvi, Syed Tatheer Alam
Lei, Fanglin
Zhang, Zhenyu
Yu, Haiying
Ma, Luyan Z.
Glycosyl hydrolase from Pseudomonas fluorescens inhibits the biofilm formation of Pseudomonads
title Glycosyl hydrolase from Pseudomonas fluorescens inhibits the biofilm formation of Pseudomonads
title_full Glycosyl hydrolase from Pseudomonas fluorescens inhibits the biofilm formation of Pseudomonads
title_fullStr Glycosyl hydrolase from Pseudomonas fluorescens inhibits the biofilm formation of Pseudomonads
title_full_unstemmed Glycosyl hydrolase from Pseudomonas fluorescens inhibits the biofilm formation of Pseudomonads
title_short Glycosyl hydrolase from Pseudomonas fluorescens inhibits the biofilm formation of Pseudomonads
title_sort glycosyl hydrolase from pseudomonas fluorescens inhibits the biofilm formation of pseudomonads
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10622837/
https://www.ncbi.nlm.nih.gov/pubmed/37928620
http://dx.doi.org/10.1016/j.bioflm.2023.100155
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