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The fibronectin concentration that optimally maintains porcine satellite cells

OBJECTIVE: ‘Cultured meat’ has been suggested as means of solving the problems associated with overpopulation and gas emissions. Satellite cells are a major component in the production of cultured meat; however, these cells cannot be maintained in vitro over long periods. Fibronectin is a glycoprote...

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Detalles Bibliográficos
Autores principales: Han, Jae Ho, Jang, Si Won, Kim, Ye Rim, Jang, Hoon, Shim, Kwan Seob, Choi, Hyun Woo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Animal Bioscience 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10623030/
https://www.ncbi.nlm.nih.gov/pubmed/37592381
http://dx.doi.org/10.5713/ab.23.0108
Descripción
Sumario:OBJECTIVE: ‘Cultured meat’ has been suggested as means of solving the problems associated with overpopulation and gas emissions. Satellite cells are a major component in the production of cultured meat; however, these cells cannot be maintained in vitro over long periods. Fibronectin is a glycoprotein that affects biological processes such as cell adhesion, differentiation, and migration. Unfortunately, the characteristics of porcine satellite cells grown in a long-term culture when exposed to fibronectin-coated dishes are unknown. The objective of this study was to investigate the appropriate concentration of fibronectin coated dishes for proliferation and maintenance of porcine satellite cells at long-term culture. METHODS: In this study, we isolated the satellite cells and fibroblast cells with pre-plating method. We next analyzed the cell doubling time, cell cycle, and rate of expressed paired box 7 (Pax7) and myogenic differentiation 1 (MyoD1) in porcine satellite cells cultured with 20 μg/mL of fibronectin-, gelatin-, and non-coated dishes at early and late passage. We then analyzed the proliferation of porcine satellite cells with various concentrations of mixed gelatin/fibronectin. We next determined the optimal concentration of fibronectin that would encourage proliferation and maintenance of porcine satellite cells in a long-term culture. RESULTS: Doubling time was lowest when 20 μg/mL of fibronectin was used (as tested during an early and late passage). Levels of expressed Pax7 and MyoD1, assessed using immunocytochemistry, were highest in cells grown using fibronectin-coated dishes. The proliferation of gelatin/fibronectin mixed coatings had no significant effect on porcine satellite cells. The concentration of 5 μg/mL fibronectin coated dishes showed the lowest doubling time and maintained expression of Pax7. CONCLUSION: Fibronectin with 5μg/mL effectively maintains porcine satellite cells, a discovery that will be of interest to those developing the next generation of artificial meats.