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The fibronectin concentration that optimally maintains porcine satellite cells

OBJECTIVE: ‘Cultured meat’ has been suggested as means of solving the problems associated with overpopulation and gas emissions. Satellite cells are a major component in the production of cultured meat; however, these cells cannot be maintained in vitro over long periods. Fibronectin is a glycoprote...

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Autores principales: Han, Jae Ho, Jang, Si Won, Kim, Ye Rim, Jang, Hoon, Shim, Kwan Seob, Choi, Hyun Woo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Animal Bioscience 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10623030/
https://www.ncbi.nlm.nih.gov/pubmed/37592381
http://dx.doi.org/10.5713/ab.23.0108
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author Han, Jae Ho
Jang, Si Won
Kim, Ye Rim
Jang, Hoon
Shim, Kwan Seob
Choi, Hyun Woo
author_facet Han, Jae Ho
Jang, Si Won
Kim, Ye Rim
Jang, Hoon
Shim, Kwan Seob
Choi, Hyun Woo
author_sort Han, Jae Ho
collection PubMed
description OBJECTIVE: ‘Cultured meat’ has been suggested as means of solving the problems associated with overpopulation and gas emissions. Satellite cells are a major component in the production of cultured meat; however, these cells cannot be maintained in vitro over long periods. Fibronectin is a glycoprotein that affects biological processes such as cell adhesion, differentiation, and migration. Unfortunately, the characteristics of porcine satellite cells grown in a long-term culture when exposed to fibronectin-coated dishes are unknown. The objective of this study was to investigate the appropriate concentration of fibronectin coated dishes for proliferation and maintenance of porcine satellite cells at long-term culture. METHODS: In this study, we isolated the satellite cells and fibroblast cells with pre-plating method. We next analyzed the cell doubling time, cell cycle, and rate of expressed paired box 7 (Pax7) and myogenic differentiation 1 (MyoD1) in porcine satellite cells cultured with 20 μg/mL of fibronectin-, gelatin-, and non-coated dishes at early and late passage. We then analyzed the proliferation of porcine satellite cells with various concentrations of mixed gelatin/fibronectin. We next determined the optimal concentration of fibronectin that would encourage proliferation and maintenance of porcine satellite cells in a long-term culture. RESULTS: Doubling time was lowest when 20 μg/mL of fibronectin was used (as tested during an early and late passage). Levels of expressed Pax7 and MyoD1, assessed using immunocytochemistry, were highest in cells grown using fibronectin-coated dishes. The proliferation of gelatin/fibronectin mixed coatings had no significant effect on porcine satellite cells. The concentration of 5 μg/mL fibronectin coated dishes showed the lowest doubling time and maintained expression of Pax7. CONCLUSION: Fibronectin with 5μg/mL effectively maintains porcine satellite cells, a discovery that will be of interest to those developing the next generation of artificial meats.
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spelling pubmed-106230302023-12-01 The fibronectin concentration that optimally maintains porcine satellite cells Han, Jae Ho Jang, Si Won Kim, Ye Rim Jang, Hoon Shim, Kwan Seob Choi, Hyun Woo Anim Biosci Article OBJECTIVE: ‘Cultured meat’ has been suggested as means of solving the problems associated with overpopulation and gas emissions. Satellite cells are a major component in the production of cultured meat; however, these cells cannot be maintained in vitro over long periods. Fibronectin is a glycoprotein that affects biological processes such as cell adhesion, differentiation, and migration. Unfortunately, the characteristics of porcine satellite cells grown in a long-term culture when exposed to fibronectin-coated dishes are unknown. The objective of this study was to investigate the appropriate concentration of fibronectin coated dishes for proliferation and maintenance of porcine satellite cells at long-term culture. METHODS: In this study, we isolated the satellite cells and fibroblast cells with pre-plating method. We next analyzed the cell doubling time, cell cycle, and rate of expressed paired box 7 (Pax7) and myogenic differentiation 1 (MyoD1) in porcine satellite cells cultured with 20 μg/mL of fibronectin-, gelatin-, and non-coated dishes at early and late passage. We then analyzed the proliferation of porcine satellite cells with various concentrations of mixed gelatin/fibronectin. We next determined the optimal concentration of fibronectin that would encourage proliferation and maintenance of porcine satellite cells in a long-term culture. RESULTS: Doubling time was lowest when 20 μg/mL of fibronectin was used (as tested during an early and late passage). Levels of expressed Pax7 and MyoD1, assessed using immunocytochemistry, were highest in cells grown using fibronectin-coated dishes. The proliferation of gelatin/fibronectin mixed coatings had no significant effect on porcine satellite cells. The concentration of 5 μg/mL fibronectin coated dishes showed the lowest doubling time and maintained expression of Pax7. CONCLUSION: Fibronectin with 5μg/mL effectively maintains porcine satellite cells, a discovery that will be of interest to those developing the next generation of artificial meats. Animal Bioscience 2023-12 2023-08-16 /pmc/articles/PMC10623030/ /pubmed/37592381 http://dx.doi.org/10.5713/ab.23.0108 Text en Copyright © 2023 by Animal Bioscience https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Han, Jae Ho
Jang, Si Won
Kim, Ye Rim
Jang, Hoon
Shim, Kwan Seob
Choi, Hyun Woo
The fibronectin concentration that optimally maintains porcine satellite cells
title The fibronectin concentration that optimally maintains porcine satellite cells
title_full The fibronectin concentration that optimally maintains porcine satellite cells
title_fullStr The fibronectin concentration that optimally maintains porcine satellite cells
title_full_unstemmed The fibronectin concentration that optimally maintains porcine satellite cells
title_short The fibronectin concentration that optimally maintains porcine satellite cells
title_sort fibronectin concentration that optimally maintains porcine satellite cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10623030/
https://www.ncbi.nlm.nih.gov/pubmed/37592381
http://dx.doi.org/10.5713/ab.23.0108
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