Cargando…

Exploring microbial diversity responses in agricultural fields: a comparative analysis under pesticide stress and non-stress conditions

Exposure to pesticides changes the microbial community structure in contaminated agricultural fields. To analyze the changes in the native microbial composition qRT-PCR, a metagenomic study was conducted. The qRT-PCR results exhibited that the uncontaminated soil has a higher copy number of 16S rDNA...

Descripción completa

Detalles Bibliográficos
Autores principales: Gangola, Saurabh, Joshi, Samiksha, Bhandari, Geeta, Pant, Garima, Sharma, Anita, Perveen, Kahkashan, Bukhari, Najat A., Rani, Ranjana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10623313/
https://www.ncbi.nlm.nih.gov/pubmed/37928679
http://dx.doi.org/10.3389/fmicb.2023.1271129
_version_ 1785130715406401536
author Gangola, Saurabh
Joshi, Samiksha
Bhandari, Geeta
Pant, Garima
Sharma, Anita
Perveen, Kahkashan
Bukhari, Najat A.
Rani, Ranjana
author_facet Gangola, Saurabh
Joshi, Samiksha
Bhandari, Geeta
Pant, Garima
Sharma, Anita
Perveen, Kahkashan
Bukhari, Najat A.
Rani, Ranjana
author_sort Gangola, Saurabh
collection PubMed
description Exposure to pesticides changes the microbial community structure in contaminated agricultural fields. To analyze the changes in the native microbial composition qRT-PCR, a metagenomic study was conducted. The qRT-PCR results exhibited that the uncontaminated soil has a higher copy number of 16S rDNA relative to the soil contaminated with pesticide. Metagenome analysis interprets that uncontaminated soil is enriched with proteobacteria in comparison with pesticide-contaminated soil. However, the presence of Actinobacteria, Firmicutes, and Bacteroides was found to be dominant in the pesticide-spiked soil. Additionally, the presence of new phyla such as Chloroflexi, Planctomycetes, and Verrucomicrobia was noted in the pesticide-spiked soil, while Acidobacteria and Crenarchaeota were observed to be extinct. These findings highlight that exposure to pesticides on soil significantly impacts the biological composition of the soil. The abundance of microbial composition under pesticide stress could be of better use for the treatment of biodegradation and bioremediation of pesticides in contaminated environments.
format Online
Article
Text
id pubmed-10623313
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-106233132023-11-04 Exploring microbial diversity responses in agricultural fields: a comparative analysis under pesticide stress and non-stress conditions Gangola, Saurabh Joshi, Samiksha Bhandari, Geeta Pant, Garima Sharma, Anita Perveen, Kahkashan Bukhari, Najat A. Rani, Ranjana Front Microbiol Microbiology Exposure to pesticides changes the microbial community structure in contaminated agricultural fields. To analyze the changes in the native microbial composition qRT-PCR, a metagenomic study was conducted. The qRT-PCR results exhibited that the uncontaminated soil has a higher copy number of 16S rDNA relative to the soil contaminated with pesticide. Metagenome analysis interprets that uncontaminated soil is enriched with proteobacteria in comparison with pesticide-contaminated soil. However, the presence of Actinobacteria, Firmicutes, and Bacteroides was found to be dominant in the pesticide-spiked soil. Additionally, the presence of new phyla such as Chloroflexi, Planctomycetes, and Verrucomicrobia was noted in the pesticide-spiked soil, while Acidobacteria and Crenarchaeota were observed to be extinct. These findings highlight that exposure to pesticides on soil significantly impacts the biological composition of the soil. The abundance of microbial composition under pesticide stress could be of better use for the treatment of biodegradation and bioremediation of pesticides in contaminated environments. Frontiers Media S.A. 2023-10-20 /pmc/articles/PMC10623313/ /pubmed/37928679 http://dx.doi.org/10.3389/fmicb.2023.1271129 Text en Copyright © 2023 Gangola, Joshi, Bhandari, Pant, Sharma, Perveen, Bukhari and Rani. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Gangola, Saurabh
Joshi, Samiksha
Bhandari, Geeta
Pant, Garima
Sharma, Anita
Perveen, Kahkashan
Bukhari, Najat A.
Rani, Ranjana
Exploring microbial diversity responses in agricultural fields: a comparative analysis under pesticide stress and non-stress conditions
title Exploring microbial diversity responses in agricultural fields: a comparative analysis under pesticide stress and non-stress conditions
title_full Exploring microbial diversity responses in agricultural fields: a comparative analysis under pesticide stress and non-stress conditions
title_fullStr Exploring microbial diversity responses in agricultural fields: a comparative analysis under pesticide stress and non-stress conditions
title_full_unstemmed Exploring microbial diversity responses in agricultural fields: a comparative analysis under pesticide stress and non-stress conditions
title_short Exploring microbial diversity responses in agricultural fields: a comparative analysis under pesticide stress and non-stress conditions
title_sort exploring microbial diversity responses in agricultural fields: a comparative analysis under pesticide stress and non-stress conditions
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10623313/
https://www.ncbi.nlm.nih.gov/pubmed/37928679
http://dx.doi.org/10.3389/fmicb.2023.1271129
work_keys_str_mv AT gangolasaurabh exploringmicrobialdiversityresponsesinagriculturalfieldsacomparativeanalysisunderpesticidestressandnonstressconditions
AT joshisamiksha exploringmicrobialdiversityresponsesinagriculturalfieldsacomparativeanalysisunderpesticidestressandnonstressconditions
AT bhandarigeeta exploringmicrobialdiversityresponsesinagriculturalfieldsacomparativeanalysisunderpesticidestressandnonstressconditions
AT pantgarima exploringmicrobialdiversityresponsesinagriculturalfieldsacomparativeanalysisunderpesticidestressandnonstressconditions
AT sharmaanita exploringmicrobialdiversityresponsesinagriculturalfieldsacomparativeanalysisunderpesticidestressandnonstressconditions
AT perveenkahkashan exploringmicrobialdiversityresponsesinagriculturalfieldsacomparativeanalysisunderpesticidestressandnonstressconditions
AT bukharinajata exploringmicrobialdiversityresponsesinagriculturalfieldsacomparativeanalysisunderpesticidestressandnonstressconditions
AT raniranjana exploringmicrobialdiversityresponsesinagriculturalfieldsacomparativeanalysisunderpesticidestressandnonstressconditions