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Exploring microbial diversity responses in agricultural fields: a comparative analysis under pesticide stress and non-stress conditions
Exposure to pesticides changes the microbial community structure in contaminated agricultural fields. To analyze the changes in the native microbial composition qRT-PCR, a metagenomic study was conducted. The qRT-PCR results exhibited that the uncontaminated soil has a higher copy number of 16S rDNA...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10623313/ https://www.ncbi.nlm.nih.gov/pubmed/37928679 http://dx.doi.org/10.3389/fmicb.2023.1271129 |
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author | Gangola, Saurabh Joshi, Samiksha Bhandari, Geeta Pant, Garima Sharma, Anita Perveen, Kahkashan Bukhari, Najat A. Rani, Ranjana |
author_facet | Gangola, Saurabh Joshi, Samiksha Bhandari, Geeta Pant, Garima Sharma, Anita Perveen, Kahkashan Bukhari, Najat A. Rani, Ranjana |
author_sort | Gangola, Saurabh |
collection | PubMed |
description | Exposure to pesticides changes the microbial community structure in contaminated agricultural fields. To analyze the changes in the native microbial composition qRT-PCR, a metagenomic study was conducted. The qRT-PCR results exhibited that the uncontaminated soil has a higher copy number of 16S rDNA relative to the soil contaminated with pesticide. Metagenome analysis interprets that uncontaminated soil is enriched with proteobacteria in comparison with pesticide-contaminated soil. However, the presence of Actinobacteria, Firmicutes, and Bacteroides was found to be dominant in the pesticide-spiked soil. Additionally, the presence of new phyla such as Chloroflexi, Planctomycetes, and Verrucomicrobia was noted in the pesticide-spiked soil, while Acidobacteria and Crenarchaeota were observed to be extinct. These findings highlight that exposure to pesticides on soil significantly impacts the biological composition of the soil. The abundance of microbial composition under pesticide stress could be of better use for the treatment of biodegradation and bioremediation of pesticides in contaminated environments. |
format | Online Article Text |
id | pubmed-10623313 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-106233132023-11-04 Exploring microbial diversity responses in agricultural fields: a comparative analysis under pesticide stress and non-stress conditions Gangola, Saurabh Joshi, Samiksha Bhandari, Geeta Pant, Garima Sharma, Anita Perveen, Kahkashan Bukhari, Najat A. Rani, Ranjana Front Microbiol Microbiology Exposure to pesticides changes the microbial community structure in contaminated agricultural fields. To analyze the changes in the native microbial composition qRT-PCR, a metagenomic study was conducted. The qRT-PCR results exhibited that the uncontaminated soil has a higher copy number of 16S rDNA relative to the soil contaminated with pesticide. Metagenome analysis interprets that uncontaminated soil is enriched with proteobacteria in comparison with pesticide-contaminated soil. However, the presence of Actinobacteria, Firmicutes, and Bacteroides was found to be dominant in the pesticide-spiked soil. Additionally, the presence of new phyla such as Chloroflexi, Planctomycetes, and Verrucomicrobia was noted in the pesticide-spiked soil, while Acidobacteria and Crenarchaeota were observed to be extinct. These findings highlight that exposure to pesticides on soil significantly impacts the biological composition of the soil. The abundance of microbial composition under pesticide stress could be of better use for the treatment of biodegradation and bioremediation of pesticides in contaminated environments. Frontiers Media S.A. 2023-10-20 /pmc/articles/PMC10623313/ /pubmed/37928679 http://dx.doi.org/10.3389/fmicb.2023.1271129 Text en Copyright © 2023 Gangola, Joshi, Bhandari, Pant, Sharma, Perveen, Bukhari and Rani. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Gangola, Saurabh Joshi, Samiksha Bhandari, Geeta Pant, Garima Sharma, Anita Perveen, Kahkashan Bukhari, Najat A. Rani, Ranjana Exploring microbial diversity responses in agricultural fields: a comparative analysis under pesticide stress and non-stress conditions |
title | Exploring microbial diversity responses in agricultural fields: a comparative analysis under pesticide stress and non-stress conditions |
title_full | Exploring microbial diversity responses in agricultural fields: a comparative analysis under pesticide stress and non-stress conditions |
title_fullStr | Exploring microbial diversity responses in agricultural fields: a comparative analysis under pesticide stress and non-stress conditions |
title_full_unstemmed | Exploring microbial diversity responses in agricultural fields: a comparative analysis under pesticide stress and non-stress conditions |
title_short | Exploring microbial diversity responses in agricultural fields: a comparative analysis under pesticide stress and non-stress conditions |
title_sort | exploring microbial diversity responses in agricultural fields: a comparative analysis under pesticide stress and non-stress conditions |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10623313/ https://www.ncbi.nlm.nih.gov/pubmed/37928679 http://dx.doi.org/10.3389/fmicb.2023.1271129 |
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