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Co-infection and co-localization of Kaposi sarcoma-associated herpesvirus and Epstein-Barr virus in HIV-associated Kaposi sarcoma: a case report

Kaposi sarcoma (KS), a multifocal vascular neoplasm frequently observed in HIV-positive individuals, primarily affects the skin, mucous membranes, visceral organs, and lymph nodes. KS is associated primarily with Kaposi sarcoma-associated herpesvirus (KSHV) infection. In this case report, we present...

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Autores principales: Julius, Peter, Kang, Guobin, Siyumbwa, Stepfanie, Musumali, Jane, Tso, For Yue, Ngalamika, Owen, Kaile, Trevor, Maate, Fred, Moonga, Phyllis, West, John T., Angeletti, Peter, Wood, Charles
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10623342/
https://www.ncbi.nlm.nih.gov/pubmed/37928187
http://dx.doi.org/10.3389/fcimb.2023.1270935
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author Julius, Peter
Kang, Guobin
Siyumbwa, Stepfanie
Musumali, Jane
Tso, For Yue
Ngalamika, Owen
Kaile, Trevor
Maate, Fred
Moonga, Phyllis
West, John T.
Angeletti, Peter
Wood, Charles
author_facet Julius, Peter
Kang, Guobin
Siyumbwa, Stepfanie
Musumali, Jane
Tso, For Yue
Ngalamika, Owen
Kaile, Trevor
Maate, Fred
Moonga, Phyllis
West, John T.
Angeletti, Peter
Wood, Charles
author_sort Julius, Peter
collection PubMed
description Kaposi sarcoma (KS), a multifocal vascular neoplasm frequently observed in HIV-positive individuals, primarily affects the skin, mucous membranes, visceral organs, and lymph nodes. KS is associated primarily with Kaposi sarcoma-associated herpesvirus (KSHV) infection. In this case report, we present a rare occurrence of co-infection and co-localization of KSHV and Epstein-Barr virus (EBV) in KS arising from the conjunctiva, which, to our knowledge, has not been reported previously. Immunohistochemistry (IHC), DNA polymerase chain reaction (PCR), and EBV-encoded RNA in situ hybridization (EBER-ISH) were utilized to demonstrate the presence of KSHV and EBV infection in the ocular KS lesion. Nearly all KSHV-positive cells displayed co-infection with EBV. In addition, the KS lesion revealed co-localization of KSHV Latency-Associated Nuclear Antigen (LANA) and EBV Epstein Barr virus Nuclear Antigen-1 (EBNA1) by multi-colored immunofluorescence staining with different anti-EBNA1 antibodies, indicating the possibility of interactions between these two gamma herpesviruses within the same lesion. Additional study is needed to determine whether EBV co-infection in KS is a common or an opportunistic event that might contribute to KS development and progression.
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spelling pubmed-106233422023-11-04 Co-infection and co-localization of Kaposi sarcoma-associated herpesvirus and Epstein-Barr virus in HIV-associated Kaposi sarcoma: a case report Julius, Peter Kang, Guobin Siyumbwa, Stepfanie Musumali, Jane Tso, For Yue Ngalamika, Owen Kaile, Trevor Maate, Fred Moonga, Phyllis West, John T. Angeletti, Peter Wood, Charles Front Cell Infect Microbiol Cellular and Infection Microbiology Kaposi sarcoma (KS), a multifocal vascular neoplasm frequently observed in HIV-positive individuals, primarily affects the skin, mucous membranes, visceral organs, and lymph nodes. KS is associated primarily with Kaposi sarcoma-associated herpesvirus (KSHV) infection. In this case report, we present a rare occurrence of co-infection and co-localization of KSHV and Epstein-Barr virus (EBV) in KS arising from the conjunctiva, which, to our knowledge, has not been reported previously. Immunohistochemistry (IHC), DNA polymerase chain reaction (PCR), and EBV-encoded RNA in situ hybridization (EBER-ISH) were utilized to demonstrate the presence of KSHV and EBV infection in the ocular KS lesion. Nearly all KSHV-positive cells displayed co-infection with EBV. In addition, the KS lesion revealed co-localization of KSHV Latency-Associated Nuclear Antigen (LANA) and EBV Epstein Barr virus Nuclear Antigen-1 (EBNA1) by multi-colored immunofluorescence staining with different anti-EBNA1 antibodies, indicating the possibility of interactions between these two gamma herpesviruses within the same lesion. Additional study is needed to determine whether EBV co-infection in KS is a common or an opportunistic event that might contribute to KS development and progression. Frontiers Media S.A. 2023-10-20 /pmc/articles/PMC10623342/ /pubmed/37928187 http://dx.doi.org/10.3389/fcimb.2023.1270935 Text en Copyright © 2023 Julius, Kang, Siyumbwa, Musumali, Tso, Ngalamika, Kaile, Maate, Moonga, West, Angeletti and Wood https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Julius, Peter
Kang, Guobin
Siyumbwa, Stepfanie
Musumali, Jane
Tso, For Yue
Ngalamika, Owen
Kaile, Trevor
Maate, Fred
Moonga, Phyllis
West, John T.
Angeletti, Peter
Wood, Charles
Co-infection and co-localization of Kaposi sarcoma-associated herpesvirus and Epstein-Barr virus in HIV-associated Kaposi sarcoma: a case report
title Co-infection and co-localization of Kaposi sarcoma-associated herpesvirus and Epstein-Barr virus in HIV-associated Kaposi sarcoma: a case report
title_full Co-infection and co-localization of Kaposi sarcoma-associated herpesvirus and Epstein-Barr virus in HIV-associated Kaposi sarcoma: a case report
title_fullStr Co-infection and co-localization of Kaposi sarcoma-associated herpesvirus and Epstein-Barr virus in HIV-associated Kaposi sarcoma: a case report
title_full_unstemmed Co-infection and co-localization of Kaposi sarcoma-associated herpesvirus and Epstein-Barr virus in HIV-associated Kaposi sarcoma: a case report
title_short Co-infection and co-localization of Kaposi sarcoma-associated herpesvirus and Epstein-Barr virus in HIV-associated Kaposi sarcoma: a case report
title_sort co-infection and co-localization of kaposi sarcoma-associated herpesvirus and epstein-barr virus in hiv-associated kaposi sarcoma: a case report
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10623342/
https://www.ncbi.nlm.nih.gov/pubmed/37928187
http://dx.doi.org/10.3389/fcimb.2023.1270935
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