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Global and Spatial Metabolomics of Individual Cells Using a Tapered Pneumatically Assisted nano-DESI Probe

[Image: see text] Single-cell metabolomics has the potential to reveal unique insights into intracellular mechanisms and biological processes. However, the detection of metabolites from individual cells is challenging due to their versatile chemical properties and concentrations. Here, we demonstrat...

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Autores principales: Marques, Cátia, Friedrich, Felix, Liu, Liangwen, Castoldi, Francesca, Pietrocola, Federico, Lanekoff, Ingela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10623638/
https://www.ncbi.nlm.nih.gov/pubmed/37830184
http://dx.doi.org/10.1021/jasms.3c00239
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author Marques, Cátia
Friedrich, Felix
Liu, Liangwen
Castoldi, Francesca
Pietrocola, Federico
Lanekoff, Ingela
author_facet Marques, Cátia
Friedrich, Felix
Liu, Liangwen
Castoldi, Francesca
Pietrocola, Federico
Lanekoff, Ingela
author_sort Marques, Cátia
collection PubMed
description [Image: see text] Single-cell metabolomics has the potential to reveal unique insights into intracellular mechanisms and biological processes. However, the detection of metabolites from individual cells is challenging due to their versatile chemical properties and concentrations. Here, we demonstrate a tapered probe for pneumatically assisted nanospray desorption electrospray ionization (PA nano-DESI) mass spectrometry that enables both chemical imaging of larger cells and global metabolomics of smaller 15 μm cells. Additionally, by depositing cells in predefined arrays, we show successful metabolomics from three individual INS-1 cells per minute, which enabled the acquisition of data from 479 individual cells. Several cells were used to optimize analytical conditions, and 93 or 97 cells were used to monitor metabolome alterations in INS-1 cells after exposure to a low or high glucose concentration, respectively. Our analytical approach offers insights into cellular heterogeneity and provides valuable information about cellular processes and responses in individual cells.
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spelling pubmed-106236382023-11-04 Global and Spatial Metabolomics of Individual Cells Using a Tapered Pneumatically Assisted nano-DESI Probe Marques, Cátia Friedrich, Felix Liu, Liangwen Castoldi, Francesca Pietrocola, Federico Lanekoff, Ingela J Am Soc Mass Spectrom [Image: see text] Single-cell metabolomics has the potential to reveal unique insights into intracellular mechanisms and biological processes. However, the detection of metabolites from individual cells is challenging due to their versatile chemical properties and concentrations. Here, we demonstrate a tapered probe for pneumatically assisted nanospray desorption electrospray ionization (PA nano-DESI) mass spectrometry that enables both chemical imaging of larger cells and global metabolomics of smaller 15 μm cells. Additionally, by depositing cells in predefined arrays, we show successful metabolomics from three individual INS-1 cells per minute, which enabled the acquisition of data from 479 individual cells. Several cells were used to optimize analytical conditions, and 93 or 97 cells were used to monitor metabolome alterations in INS-1 cells after exposure to a low or high glucose concentration, respectively. Our analytical approach offers insights into cellular heterogeneity and provides valuable information about cellular processes and responses in individual cells. American Chemical Society 2023-10-13 /pmc/articles/PMC10623638/ /pubmed/37830184 http://dx.doi.org/10.1021/jasms.3c00239 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Marques, Cátia
Friedrich, Felix
Liu, Liangwen
Castoldi, Francesca
Pietrocola, Federico
Lanekoff, Ingela
Global and Spatial Metabolomics of Individual Cells Using a Tapered Pneumatically Assisted nano-DESI Probe
title Global and Spatial Metabolomics of Individual Cells Using a Tapered Pneumatically Assisted nano-DESI Probe
title_full Global and Spatial Metabolomics of Individual Cells Using a Tapered Pneumatically Assisted nano-DESI Probe
title_fullStr Global and Spatial Metabolomics of Individual Cells Using a Tapered Pneumatically Assisted nano-DESI Probe
title_full_unstemmed Global and Spatial Metabolomics of Individual Cells Using a Tapered Pneumatically Assisted nano-DESI Probe
title_short Global and Spatial Metabolomics of Individual Cells Using a Tapered Pneumatically Assisted nano-DESI Probe
title_sort global and spatial metabolomics of individual cells using a tapered pneumatically assisted nano-desi probe
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10623638/
https://www.ncbi.nlm.nih.gov/pubmed/37830184
http://dx.doi.org/10.1021/jasms.3c00239
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