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Occurrence of some common carbapenemase genes in carbapenem-resistant Klebsiella pneumoniae isolates collected from clinical samples in Tabriz, northwestern Iran
OBJECTIVES: This study aimed to evaluate the antibiotic resistance patterns and prevalence of carbapenemase genes in Klebsiella pneumoniae isolates in different clinical samples from Tabriz city, northwestern Iran. RESULTS: This cross-sectional study was conducted in the Department of Microbiology,...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10623837/ https://www.ncbi.nlm.nih.gov/pubmed/37924149 http://dx.doi.org/10.1186/s13104-023-06558-x |
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author | Jafari-Sales, Abolfazl Al-Khafaji, Noor S.K. Al-Dahmoshi, Hussein O.M. Sadeghi Deylamdeh, Zahra Akrami, Sousan Shariat, Afsoon Judi, Hawraa K. Nasiri, Rozita Bannazadeh Baghi, Hossein Saki, Morteza |
author_facet | Jafari-Sales, Abolfazl Al-Khafaji, Noor S.K. Al-Dahmoshi, Hussein O.M. Sadeghi Deylamdeh, Zahra Akrami, Sousan Shariat, Afsoon Judi, Hawraa K. Nasiri, Rozita Bannazadeh Baghi, Hossein Saki, Morteza |
author_sort | Jafari-Sales, Abolfazl |
collection | PubMed |
description | OBJECTIVES: This study aimed to evaluate the antibiotic resistance patterns and prevalence of carbapenemase genes in Klebsiella pneumoniae isolates in different clinical samples from Tabriz city, northwestern Iran. RESULTS: This cross-sectional study was conducted in the Department of Microbiology, Islamic Azad University, Ahar Branch, Iran, in 2020. K. pneumoniae isolates were collected from different clinical samples, including blood, wounds, sputum, and urine. The isolates were identified using a series of standard bacteriological tests. Antibiotic resistance was determined by the disc diffusion method. The presence of bla(VIM), bla(NDM), bla(KPC), bla(OXA), and bla(IMP) genes were screened by polymerase chain reaction (PCR). A total of 100 non-duplicated K. pneumoniae isolates were collected from 57 urine samples, 27 blood samples, 13 wound samples, and 3 sputum samples. Overall, 70.0% of the samples were from inpatients, while 30.0% were from outpatients. The most resistance rate was related to ampicillin (94.0%), while the lowest resistance rate was related to imipenem (18.0%) and meropenem (20.0%). Overall, 25.0% of the isolates were carbapenem-resistant, of which 13.0% were resistant to both imipenem and meropenem. The PCR showed the total prevalence of 23.0% for carbapenemase genes, including 18.0% for bla(KPC), 3.0% for bla(VIM), 1.0% for bla(IMP), and 1.0% for bla(OXA) gene. The bla(NDM) gene was not detected in any isolate. The prevalence of carbapenemase-producing K. pneumoniae isolates was relatively lower in northwestern Iran than in other regions of the country. However, special attention should be paid to the proper use of antibiotics, particularly carbapenems, to prevent further spread of antibiotic resistance and its related genes. |
format | Online Article Text |
id | pubmed-10623837 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-106238372023-11-04 Occurrence of some common carbapenemase genes in carbapenem-resistant Klebsiella pneumoniae isolates collected from clinical samples in Tabriz, northwestern Iran Jafari-Sales, Abolfazl Al-Khafaji, Noor S.K. Al-Dahmoshi, Hussein O.M. Sadeghi Deylamdeh, Zahra Akrami, Sousan Shariat, Afsoon Judi, Hawraa K. Nasiri, Rozita Bannazadeh Baghi, Hossein Saki, Morteza BMC Res Notes Research Note OBJECTIVES: This study aimed to evaluate the antibiotic resistance patterns and prevalence of carbapenemase genes in Klebsiella pneumoniae isolates in different clinical samples from Tabriz city, northwestern Iran. RESULTS: This cross-sectional study was conducted in the Department of Microbiology, Islamic Azad University, Ahar Branch, Iran, in 2020. K. pneumoniae isolates were collected from different clinical samples, including blood, wounds, sputum, and urine. The isolates were identified using a series of standard bacteriological tests. Antibiotic resistance was determined by the disc diffusion method. The presence of bla(VIM), bla(NDM), bla(KPC), bla(OXA), and bla(IMP) genes were screened by polymerase chain reaction (PCR). A total of 100 non-duplicated K. pneumoniae isolates were collected from 57 urine samples, 27 blood samples, 13 wound samples, and 3 sputum samples. Overall, 70.0% of the samples were from inpatients, while 30.0% were from outpatients. The most resistance rate was related to ampicillin (94.0%), while the lowest resistance rate was related to imipenem (18.0%) and meropenem (20.0%). Overall, 25.0% of the isolates were carbapenem-resistant, of which 13.0% were resistant to both imipenem and meropenem. The PCR showed the total prevalence of 23.0% for carbapenemase genes, including 18.0% for bla(KPC), 3.0% for bla(VIM), 1.0% for bla(IMP), and 1.0% for bla(OXA) gene. The bla(NDM) gene was not detected in any isolate. The prevalence of carbapenemase-producing K. pneumoniae isolates was relatively lower in northwestern Iran than in other regions of the country. However, special attention should be paid to the proper use of antibiotics, particularly carbapenems, to prevent further spread of antibiotic resistance and its related genes. BioMed Central 2023-11-03 /pmc/articles/PMC10623837/ /pubmed/37924149 http://dx.doi.org/10.1186/s13104-023-06558-x Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Note Jafari-Sales, Abolfazl Al-Khafaji, Noor S.K. Al-Dahmoshi, Hussein O.M. Sadeghi Deylamdeh, Zahra Akrami, Sousan Shariat, Afsoon Judi, Hawraa K. Nasiri, Rozita Bannazadeh Baghi, Hossein Saki, Morteza Occurrence of some common carbapenemase genes in carbapenem-resistant Klebsiella pneumoniae isolates collected from clinical samples in Tabriz, northwestern Iran |
title | Occurrence of some common carbapenemase genes in carbapenem-resistant Klebsiella pneumoniae isolates collected from clinical samples in Tabriz, northwestern Iran |
title_full | Occurrence of some common carbapenemase genes in carbapenem-resistant Klebsiella pneumoniae isolates collected from clinical samples in Tabriz, northwestern Iran |
title_fullStr | Occurrence of some common carbapenemase genes in carbapenem-resistant Klebsiella pneumoniae isolates collected from clinical samples in Tabriz, northwestern Iran |
title_full_unstemmed | Occurrence of some common carbapenemase genes in carbapenem-resistant Klebsiella pneumoniae isolates collected from clinical samples in Tabriz, northwestern Iran |
title_short | Occurrence of some common carbapenemase genes in carbapenem-resistant Klebsiella pneumoniae isolates collected from clinical samples in Tabriz, northwestern Iran |
title_sort | occurrence of some common carbapenemase genes in carbapenem-resistant klebsiella pneumoniae isolates collected from clinical samples in tabriz, northwestern iran |
topic | Research Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10623837/ https://www.ncbi.nlm.nih.gov/pubmed/37924149 http://dx.doi.org/10.1186/s13104-023-06558-x |
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