Cargando…
Single‐cell RNA sequencing reveals new subtypes of lens superficial tissue in humans
Although the cell atlas of the human ocular anterior segment of the human eye was revealed by single‐nucleus RNA sequencing, whether subtypes of lens stem/progenitor cells exist among epithelial cells and the molecular characteristics of cell differentiation of the human lens remain unclear. Single‐...
Autores principales: | , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10623935/ https://www.ncbi.nlm.nih.gov/pubmed/37057399 http://dx.doi.org/10.1111/cpr.13477 |
_version_ | 1785130838025830400 |
---|---|
author | Zhu, Meng‐Chao Hu, Wei Lin, Lei Yang, Qing‐Wen Zhang, Lu Xu, Jia‐Lin Xu, Yi‐Tong Liu, Jia‐Sheng Zhang, Meng‐Di Tong, Xiao‐Yu Zhu, Kai‐Yi Feng, Ke Feng, Yi Su, Jian‐Zhong Huang, Xiu‐Feng Li, Jin |
author_facet | Zhu, Meng‐Chao Hu, Wei Lin, Lei Yang, Qing‐Wen Zhang, Lu Xu, Jia‐Lin Xu, Yi‐Tong Liu, Jia‐Sheng Zhang, Meng‐Di Tong, Xiao‐Yu Zhu, Kai‐Yi Feng, Ke Feng, Yi Su, Jian‐Zhong Huang, Xiu‐Feng Li, Jin |
author_sort | Zhu, Meng‐Chao |
collection | PubMed |
description | Although the cell atlas of the human ocular anterior segment of the human eye was revealed by single‐nucleus RNA sequencing, whether subtypes of lens stem/progenitor cells exist among epithelial cells and the molecular characteristics of cell differentiation of the human lens remain unclear. Single‐cell RNA sequencing is a powerful tool to analyse the heterogeneity of tissues at the single cell level, leading to a better understanding of the processes of cell differentiation. By profiling 18,596 cells in human lens superficial tissue through single‐cell sequencing, we identified two subtypes of lens epithelial cells that specifically expressed C8orf4 and ADAMTSL4 with distinct spatial localization, a new type of fibre cells located directly adjacent to the epithelium, and a subpopulation of ADAMTSL4(+) cells that might be lens epithelial stem/progenitor cells. We also found two trajectories of lens epithelial cell differentiation and changes of some important genes during differentiation. |
format | Online Article Text |
id | pubmed-10623935 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-106239352023-11-04 Single‐cell RNA sequencing reveals new subtypes of lens superficial tissue in humans Zhu, Meng‐Chao Hu, Wei Lin, Lei Yang, Qing‐Wen Zhang, Lu Xu, Jia‐Lin Xu, Yi‐Tong Liu, Jia‐Sheng Zhang, Meng‐Di Tong, Xiao‐Yu Zhu, Kai‐Yi Feng, Ke Feng, Yi Su, Jian‐Zhong Huang, Xiu‐Feng Li, Jin Cell Prolif Original Articles Although the cell atlas of the human ocular anterior segment of the human eye was revealed by single‐nucleus RNA sequencing, whether subtypes of lens stem/progenitor cells exist among epithelial cells and the molecular characteristics of cell differentiation of the human lens remain unclear. Single‐cell RNA sequencing is a powerful tool to analyse the heterogeneity of tissues at the single cell level, leading to a better understanding of the processes of cell differentiation. By profiling 18,596 cells in human lens superficial tissue through single‐cell sequencing, we identified two subtypes of lens epithelial cells that specifically expressed C8orf4 and ADAMTSL4 with distinct spatial localization, a new type of fibre cells located directly adjacent to the epithelium, and a subpopulation of ADAMTSL4(+) cells that might be lens epithelial stem/progenitor cells. We also found two trajectories of lens epithelial cell differentiation and changes of some important genes during differentiation. John Wiley and Sons Inc. 2023-04-14 /pmc/articles/PMC10623935/ /pubmed/37057399 http://dx.doi.org/10.1111/cpr.13477 Text en © 2023 The Authors. Cell Proliferation published by Beijing Institute for Stem Cell and Regenerative Medicine and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Zhu, Meng‐Chao Hu, Wei Lin, Lei Yang, Qing‐Wen Zhang, Lu Xu, Jia‐Lin Xu, Yi‐Tong Liu, Jia‐Sheng Zhang, Meng‐Di Tong, Xiao‐Yu Zhu, Kai‐Yi Feng, Ke Feng, Yi Su, Jian‐Zhong Huang, Xiu‐Feng Li, Jin Single‐cell RNA sequencing reveals new subtypes of lens superficial tissue in humans |
title | Single‐cell RNA sequencing reveals new subtypes of lens superficial tissue in humans |
title_full | Single‐cell RNA sequencing reveals new subtypes of lens superficial tissue in humans |
title_fullStr | Single‐cell RNA sequencing reveals new subtypes of lens superficial tissue in humans |
title_full_unstemmed | Single‐cell RNA sequencing reveals new subtypes of lens superficial tissue in humans |
title_short | Single‐cell RNA sequencing reveals new subtypes of lens superficial tissue in humans |
title_sort | single‐cell rna sequencing reveals new subtypes of lens superficial tissue in humans |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10623935/ https://www.ncbi.nlm.nih.gov/pubmed/37057399 http://dx.doi.org/10.1111/cpr.13477 |
work_keys_str_mv | AT zhumengchao singlecellrnasequencingrevealsnewsubtypesoflenssuperficialtissueinhumans AT huwei singlecellrnasequencingrevealsnewsubtypesoflenssuperficialtissueinhumans AT linlei singlecellrnasequencingrevealsnewsubtypesoflenssuperficialtissueinhumans AT yangqingwen singlecellrnasequencingrevealsnewsubtypesoflenssuperficialtissueinhumans AT zhanglu singlecellrnasequencingrevealsnewsubtypesoflenssuperficialtissueinhumans AT xujialin singlecellrnasequencingrevealsnewsubtypesoflenssuperficialtissueinhumans AT xuyitong singlecellrnasequencingrevealsnewsubtypesoflenssuperficialtissueinhumans AT liujiasheng singlecellrnasequencingrevealsnewsubtypesoflenssuperficialtissueinhumans AT zhangmengdi singlecellrnasequencingrevealsnewsubtypesoflenssuperficialtissueinhumans AT tongxiaoyu singlecellrnasequencingrevealsnewsubtypesoflenssuperficialtissueinhumans AT zhukaiyi singlecellrnasequencingrevealsnewsubtypesoflenssuperficialtissueinhumans AT fengke singlecellrnasequencingrevealsnewsubtypesoflenssuperficialtissueinhumans AT fengyi singlecellrnasequencingrevealsnewsubtypesoflenssuperficialtissueinhumans AT sujianzhong singlecellrnasequencingrevealsnewsubtypesoflenssuperficialtissueinhumans AT huangxiufeng singlecellrnasequencingrevealsnewsubtypesoflenssuperficialtissueinhumans AT lijin singlecellrnasequencingrevealsnewsubtypesoflenssuperficialtissueinhumans |