A 2,7-dichlorofluorescein derivative to monitor microcalcifications

Herein, we report the crystal structure of 2,7-dichlorofluorescein methyl ester (DCF-ME) and its fluorescence response to hydroxyapatite binding. The reported fluorophore is very selective for staining the bone matrix and provides turn-on fluorescence upon hydroxyapatite binding. The reported fluoro...

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Detalles Bibliográficos
Autores principales: Tholen, Patrik, Brown, Connor N., Keil, Claudia, Bayir, Ali, Zeng, Hui-Hui, Haase, Hajo, Thompson, Richard B., Lengyel, Imre, Yücesan, Gündoğ
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10624163/
https://www.ncbi.nlm.nih.gov/pubmed/37927331
http://dx.doi.org/10.1039/d2me00185c
Descripción
Sumario:Herein, we report the crystal structure of 2,7-dichlorofluorescein methyl ester (DCF-ME) and its fluorescence response to hydroxyapatite binding. The reported fluorophore is very selective for staining the bone matrix and provides turn-on fluorescence upon hydroxyapatite binding. The reported fluorophore can readily pass the cell membrane of the C2C12 cell line, and it is non-toxic for the cell line. The reported fluorophore DCF-ME may find applications in monitoring bone remodeling and microcalcification as an early diagnosis tool for breast cancer and age-related macular degeneration.

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