A 2,7-dichlorofluorescein derivative to monitor microcalcifications

Herein, we report the crystal structure of 2,7-dichlorofluorescein methyl ester (DCF-ME) and its fluorescence response to hydroxyapatite binding. The reported fluorophore is very selective for staining the bone matrix and provides turn-on fluorescence upon hydroxyapatite binding. The reported fluoro...

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Autores principales: Tholen, Patrik, Brown, Connor N., Keil, Claudia, Bayir, Ali, Zeng, Hui-Hui, Haase, Hajo, Thompson, Richard B., Lengyel, Imre, Yücesan, Gündoğ
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10624163/
https://www.ncbi.nlm.nih.gov/pubmed/37927331
http://dx.doi.org/10.1039/d2me00185c
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author Tholen, Patrik
Brown, Connor N.
Keil, Claudia
Bayir, Ali
Zeng, Hui-Hui
Haase, Hajo
Thompson, Richard B.
Lengyel, Imre
Yücesan, Gündoğ
author_facet Tholen, Patrik
Brown, Connor N.
Keil, Claudia
Bayir, Ali
Zeng, Hui-Hui
Haase, Hajo
Thompson, Richard B.
Lengyel, Imre
Yücesan, Gündoğ
author_sort Tholen, Patrik
collection PubMed
description Herein, we report the crystal structure of 2,7-dichlorofluorescein methyl ester (DCF-ME) and its fluorescence response to hydroxyapatite binding. The reported fluorophore is very selective for staining the bone matrix and provides turn-on fluorescence upon hydroxyapatite binding. The reported fluorophore can readily pass the cell membrane of the C2C12 cell line, and it is non-toxic for the cell line. The reported fluorophore DCF-ME may find applications in monitoring bone remodeling and microcalcification as an early diagnosis tool for breast cancer and age-related macular degeneration.
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spelling pubmed-106241632023-11-03 A 2,7-dichlorofluorescein derivative to monitor microcalcifications Tholen, Patrik Brown, Connor N. Keil, Claudia Bayir, Ali Zeng, Hui-Hui Haase, Hajo Thompson, Richard B. Lengyel, Imre Yücesan, Gündoğ Mol Syst Des Eng Article Herein, we report the crystal structure of 2,7-dichlorofluorescein methyl ester (DCF-ME) and its fluorescence response to hydroxyapatite binding. The reported fluorophore is very selective for staining the bone matrix and provides turn-on fluorescence upon hydroxyapatite binding. The reported fluorophore can readily pass the cell membrane of the C2C12 cell line, and it is non-toxic for the cell line. The reported fluorophore DCF-ME may find applications in monitoring bone remodeling and microcalcification as an early diagnosis tool for breast cancer and age-related macular degeneration. 2022-11-01 2022-10-10 /pmc/articles/PMC10624163/ /pubmed/37927331 http://dx.doi.org/10.1039/d2me00185c Text en https://creativecommons.org/licenses/by-nc/3.0/This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.
spellingShingle Article
Tholen, Patrik
Brown, Connor N.
Keil, Claudia
Bayir, Ali
Zeng, Hui-Hui
Haase, Hajo
Thompson, Richard B.
Lengyel, Imre
Yücesan, Gündoğ
A 2,7-dichlorofluorescein derivative to monitor microcalcifications
title A 2,7-dichlorofluorescein derivative to monitor microcalcifications
title_full A 2,7-dichlorofluorescein derivative to monitor microcalcifications
title_fullStr A 2,7-dichlorofluorescein derivative to monitor microcalcifications
title_full_unstemmed A 2,7-dichlorofluorescein derivative to monitor microcalcifications
title_short A 2,7-dichlorofluorescein derivative to monitor microcalcifications
title_sort 2,7-dichlorofluorescein derivative to monitor microcalcifications
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10624163/
https://www.ncbi.nlm.nih.gov/pubmed/37927331
http://dx.doi.org/10.1039/d2me00185c
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