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Vibrio cholerae’s ToxRS bile sensing system

The seventh pandemic of the diarrheal cholera disease, which began in 1960, is caused by the Gram-negative bacterium Vibrio cholerae. Its environmental persistence provoking recurring sudden outbreaks is enabled by V. cholerae’s rapid adaption to changing environments involving sensory proteins like...

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Autores principales: Gubensäk, Nina, Sagmeister, Theo, Buhlheller, Christoph, Geronimo, Bruno Di, Wagner, Gabriel E, Petrowitsch, Lukas, Gräwert, Melissa A, Rotzinger, Markus, Berger, Tamara M Ismael, Schäfer, Jan, Usón, Isabel, Reidl, Joachim, Sánchez-Murcia, Pedro A, Zangger, Klaus, Pavkov-Keller, Tea
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10624426/
https://www.ncbi.nlm.nih.gov/pubmed/37768326
http://dx.doi.org/10.7554/eLife.88721
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author Gubensäk, Nina
Sagmeister, Theo
Buhlheller, Christoph
Geronimo, Bruno Di
Wagner, Gabriel E
Petrowitsch, Lukas
Gräwert, Melissa A
Rotzinger, Markus
Berger, Tamara M Ismael
Schäfer, Jan
Usón, Isabel
Reidl, Joachim
Sánchez-Murcia, Pedro A
Zangger, Klaus
Pavkov-Keller, Tea
author_facet Gubensäk, Nina
Sagmeister, Theo
Buhlheller, Christoph
Geronimo, Bruno Di
Wagner, Gabriel E
Petrowitsch, Lukas
Gräwert, Melissa A
Rotzinger, Markus
Berger, Tamara M Ismael
Schäfer, Jan
Usón, Isabel
Reidl, Joachim
Sánchez-Murcia, Pedro A
Zangger, Klaus
Pavkov-Keller, Tea
author_sort Gubensäk, Nina
collection PubMed
description The seventh pandemic of the diarrheal cholera disease, which began in 1960, is caused by the Gram-negative bacterium Vibrio cholerae. Its environmental persistence provoking recurring sudden outbreaks is enabled by V. cholerae’s rapid adaption to changing environments involving sensory proteins like ToxR and ToxS. Located at the inner membrane, ToxR and ToxS react to environmental stimuli like bile acid, thereby inducing survival strategies for example bile resistance and virulence regulation. The presented crystal structure of the sensory domains of ToxR and ToxS in combination with multiple bile acid interaction studies, reveals that a bile binding pocket of ToxS is only properly folded upon binding to ToxR. Our data proposes an interdependent functionality between ToxR transcriptional activity and ToxS sensory function. These findings support the previously suggested link between ToxRS and VtrAC-like co-component systems. Besides VtrAC, ToxRS is now the only experimentally determined structure within this recently defined superfamily, further emphasizing its significance. In-depth analysis of the ToxRS complex reveals its remarkable conservation across various Vibrio species, underlining the significance of conserved residues in the ToxS barrel and the more diverse ToxR sensory domain. Unravelling the intricate mechanisms governing ToxRS’s environmental sensing capabilities, provides a promising tool for disruption of this vital interaction, ultimately inhibiting Vibrio’s survival and virulence. Our findings hold far-reaching implications for all Vibrio strains that rely on the ToxRS system as a shared sensory cornerstone for adapting to their surroundings.
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spelling pubmed-106244262023-11-04 Vibrio cholerae’s ToxRS bile sensing system Gubensäk, Nina Sagmeister, Theo Buhlheller, Christoph Geronimo, Bruno Di Wagner, Gabriel E Petrowitsch, Lukas Gräwert, Melissa A Rotzinger, Markus Berger, Tamara M Ismael Schäfer, Jan Usón, Isabel Reidl, Joachim Sánchez-Murcia, Pedro A Zangger, Klaus Pavkov-Keller, Tea eLife Biochemistry and Chemical Biology The seventh pandemic of the diarrheal cholera disease, which began in 1960, is caused by the Gram-negative bacterium Vibrio cholerae. Its environmental persistence provoking recurring sudden outbreaks is enabled by V. cholerae’s rapid adaption to changing environments involving sensory proteins like ToxR and ToxS. Located at the inner membrane, ToxR and ToxS react to environmental stimuli like bile acid, thereby inducing survival strategies for example bile resistance and virulence regulation. The presented crystal structure of the sensory domains of ToxR and ToxS in combination with multiple bile acid interaction studies, reveals that a bile binding pocket of ToxS is only properly folded upon binding to ToxR. Our data proposes an interdependent functionality between ToxR transcriptional activity and ToxS sensory function. These findings support the previously suggested link between ToxRS and VtrAC-like co-component systems. Besides VtrAC, ToxRS is now the only experimentally determined structure within this recently defined superfamily, further emphasizing its significance. In-depth analysis of the ToxRS complex reveals its remarkable conservation across various Vibrio species, underlining the significance of conserved residues in the ToxS barrel and the more diverse ToxR sensory domain. Unravelling the intricate mechanisms governing ToxRS’s environmental sensing capabilities, provides a promising tool for disruption of this vital interaction, ultimately inhibiting Vibrio’s survival and virulence. Our findings hold far-reaching implications for all Vibrio strains that rely on the ToxRS system as a shared sensory cornerstone for adapting to their surroundings. eLife Sciences Publications, Ltd 2023-09-28 /pmc/articles/PMC10624426/ /pubmed/37768326 http://dx.doi.org/10.7554/eLife.88721 Text en © 2023, Gubensäk et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Biochemistry and Chemical Biology
Gubensäk, Nina
Sagmeister, Theo
Buhlheller, Christoph
Geronimo, Bruno Di
Wagner, Gabriel E
Petrowitsch, Lukas
Gräwert, Melissa A
Rotzinger, Markus
Berger, Tamara M Ismael
Schäfer, Jan
Usón, Isabel
Reidl, Joachim
Sánchez-Murcia, Pedro A
Zangger, Klaus
Pavkov-Keller, Tea
Vibrio cholerae’s ToxRS bile sensing system
title Vibrio cholerae’s ToxRS bile sensing system
title_full Vibrio cholerae’s ToxRS bile sensing system
title_fullStr Vibrio cholerae’s ToxRS bile sensing system
title_full_unstemmed Vibrio cholerae’s ToxRS bile sensing system
title_short Vibrio cholerae’s ToxRS bile sensing system
title_sort vibrio cholerae’s toxrs bile sensing system
topic Biochemistry and Chemical Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10624426/
https://www.ncbi.nlm.nih.gov/pubmed/37768326
http://dx.doi.org/10.7554/eLife.88721
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