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Vibrio cholerae’s ToxRS bile sensing system
The seventh pandemic of the diarrheal cholera disease, which began in 1960, is caused by the Gram-negative bacterium Vibrio cholerae. Its environmental persistence provoking recurring sudden outbreaks is enabled by V. cholerae’s rapid adaption to changing environments involving sensory proteins like...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10624426/ https://www.ncbi.nlm.nih.gov/pubmed/37768326 http://dx.doi.org/10.7554/eLife.88721 |
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author | Gubensäk, Nina Sagmeister, Theo Buhlheller, Christoph Geronimo, Bruno Di Wagner, Gabriel E Petrowitsch, Lukas Gräwert, Melissa A Rotzinger, Markus Berger, Tamara M Ismael Schäfer, Jan Usón, Isabel Reidl, Joachim Sánchez-Murcia, Pedro A Zangger, Klaus Pavkov-Keller, Tea |
author_facet | Gubensäk, Nina Sagmeister, Theo Buhlheller, Christoph Geronimo, Bruno Di Wagner, Gabriel E Petrowitsch, Lukas Gräwert, Melissa A Rotzinger, Markus Berger, Tamara M Ismael Schäfer, Jan Usón, Isabel Reidl, Joachim Sánchez-Murcia, Pedro A Zangger, Klaus Pavkov-Keller, Tea |
author_sort | Gubensäk, Nina |
collection | PubMed |
description | The seventh pandemic of the diarrheal cholera disease, which began in 1960, is caused by the Gram-negative bacterium Vibrio cholerae. Its environmental persistence provoking recurring sudden outbreaks is enabled by V. cholerae’s rapid adaption to changing environments involving sensory proteins like ToxR and ToxS. Located at the inner membrane, ToxR and ToxS react to environmental stimuli like bile acid, thereby inducing survival strategies for example bile resistance and virulence regulation. The presented crystal structure of the sensory domains of ToxR and ToxS in combination with multiple bile acid interaction studies, reveals that a bile binding pocket of ToxS is only properly folded upon binding to ToxR. Our data proposes an interdependent functionality between ToxR transcriptional activity and ToxS sensory function. These findings support the previously suggested link between ToxRS and VtrAC-like co-component systems. Besides VtrAC, ToxRS is now the only experimentally determined structure within this recently defined superfamily, further emphasizing its significance. In-depth analysis of the ToxRS complex reveals its remarkable conservation across various Vibrio species, underlining the significance of conserved residues in the ToxS barrel and the more diverse ToxR sensory domain. Unravelling the intricate mechanisms governing ToxRS’s environmental sensing capabilities, provides a promising tool for disruption of this vital interaction, ultimately inhibiting Vibrio’s survival and virulence. Our findings hold far-reaching implications for all Vibrio strains that rely on the ToxRS system as a shared sensory cornerstone for adapting to their surroundings. |
format | Online Article Text |
id | pubmed-10624426 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-106244262023-11-04 Vibrio cholerae’s ToxRS bile sensing system Gubensäk, Nina Sagmeister, Theo Buhlheller, Christoph Geronimo, Bruno Di Wagner, Gabriel E Petrowitsch, Lukas Gräwert, Melissa A Rotzinger, Markus Berger, Tamara M Ismael Schäfer, Jan Usón, Isabel Reidl, Joachim Sánchez-Murcia, Pedro A Zangger, Klaus Pavkov-Keller, Tea eLife Biochemistry and Chemical Biology The seventh pandemic of the diarrheal cholera disease, which began in 1960, is caused by the Gram-negative bacterium Vibrio cholerae. Its environmental persistence provoking recurring sudden outbreaks is enabled by V. cholerae’s rapid adaption to changing environments involving sensory proteins like ToxR and ToxS. Located at the inner membrane, ToxR and ToxS react to environmental stimuli like bile acid, thereby inducing survival strategies for example bile resistance and virulence regulation. The presented crystal structure of the sensory domains of ToxR and ToxS in combination with multiple bile acid interaction studies, reveals that a bile binding pocket of ToxS is only properly folded upon binding to ToxR. Our data proposes an interdependent functionality between ToxR transcriptional activity and ToxS sensory function. These findings support the previously suggested link between ToxRS and VtrAC-like co-component systems. Besides VtrAC, ToxRS is now the only experimentally determined structure within this recently defined superfamily, further emphasizing its significance. In-depth analysis of the ToxRS complex reveals its remarkable conservation across various Vibrio species, underlining the significance of conserved residues in the ToxS barrel and the more diverse ToxR sensory domain. Unravelling the intricate mechanisms governing ToxRS’s environmental sensing capabilities, provides a promising tool for disruption of this vital interaction, ultimately inhibiting Vibrio’s survival and virulence. Our findings hold far-reaching implications for all Vibrio strains that rely on the ToxRS system as a shared sensory cornerstone for adapting to their surroundings. eLife Sciences Publications, Ltd 2023-09-28 /pmc/articles/PMC10624426/ /pubmed/37768326 http://dx.doi.org/10.7554/eLife.88721 Text en © 2023, Gubensäk et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Biochemistry and Chemical Biology Gubensäk, Nina Sagmeister, Theo Buhlheller, Christoph Geronimo, Bruno Di Wagner, Gabriel E Petrowitsch, Lukas Gräwert, Melissa A Rotzinger, Markus Berger, Tamara M Ismael Schäfer, Jan Usón, Isabel Reidl, Joachim Sánchez-Murcia, Pedro A Zangger, Klaus Pavkov-Keller, Tea Vibrio cholerae’s ToxRS bile sensing system |
title | Vibrio cholerae’s ToxRS bile sensing system |
title_full | Vibrio cholerae’s ToxRS bile sensing system |
title_fullStr | Vibrio cholerae’s ToxRS bile sensing system |
title_full_unstemmed | Vibrio cholerae’s ToxRS bile sensing system |
title_short | Vibrio cholerae’s ToxRS bile sensing system |
title_sort | vibrio cholerae’s toxrs bile sensing system |
topic | Biochemistry and Chemical Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10624426/ https://www.ncbi.nlm.nih.gov/pubmed/37768326 http://dx.doi.org/10.7554/eLife.88721 |
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