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Optical control of ultrafast structural dynamics in a fluorescent protein

The photoisomerization reaction of a fluorescent protein chromophore occurs on the ultrafast timescale. The structural dynamics that result from femtosecond optical excitation have contributions from vibrational and electronic processes and from reaction dynamics that involve the crossing through a...

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Autores principales: Hutchison, Christopher D. M., Baxter, James M., Fitzpatrick, Ann, Dorlhiac, Gabriel, Fadini, Alisia, Perrett, Samuel, Maghlaoui, Karim, Lefèvre, Salomé Bodet, Cordon-Preciado, Violeta, Ferreira, Josie L., Chukhutsina, Volha U., Garratt, Douglas, Barnard, Jonathan, Galinis, Gediminas, Glencross, Flo, Morgan, Rhodri M., Stockton, Sian, Taylor, Ben, Yuan, Letong, Romei, Matthew G., Lin, Chi-Yun, Marangos, Jon P., Schmidt, Marius, Chatrchyan, Viktoria, Buckup, Tiago, Morozov, Dmitry, Park, Jaehyun, Park, Sehan, Eom, Intae, Kim, Minseok, Jang, Dogeun, Choi, Hyeongi, Hyun, HyoJung, Park, Gisu, Nango, Eriko, Tanaka, Rie, Owada, Shigeki, Tono, Kensuke, DePonte, Daniel P., Carbajo, Sergio, Seaberg, Matt, Aquila, Andrew, Boutet, Sebastien, Barty, Anton, Iwata, So, Boxer, Steven G., Groenhof, Gerrit, van Thor, Jasper J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10624617/
https://www.ncbi.nlm.nih.gov/pubmed/37563326
http://dx.doi.org/10.1038/s41557-023-01275-1
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author Hutchison, Christopher D. M.
Baxter, James M.
Fitzpatrick, Ann
Dorlhiac, Gabriel
Fadini, Alisia
Perrett, Samuel
Maghlaoui, Karim
Lefèvre, Salomé Bodet
Cordon-Preciado, Violeta
Ferreira, Josie L.
Chukhutsina, Volha U.
Garratt, Douglas
Barnard, Jonathan
Galinis, Gediminas
Glencross, Flo
Morgan, Rhodri M.
Stockton, Sian
Taylor, Ben
Yuan, Letong
Romei, Matthew G.
Lin, Chi-Yun
Marangos, Jon P.
Schmidt, Marius
Chatrchyan, Viktoria
Buckup, Tiago
Morozov, Dmitry
Park, Jaehyun
Park, Sehan
Eom, Intae
Kim, Minseok
Jang, Dogeun
Choi, Hyeongi
Hyun, HyoJung
Park, Gisu
Nango, Eriko
Tanaka, Rie
Owada, Shigeki
Tono, Kensuke
DePonte, Daniel P.
Carbajo, Sergio
Seaberg, Matt
Aquila, Andrew
Boutet, Sebastien
Barty, Anton
Iwata, So
Boxer, Steven G.
Groenhof, Gerrit
van Thor, Jasper J.
author_facet Hutchison, Christopher D. M.
Baxter, James M.
Fitzpatrick, Ann
Dorlhiac, Gabriel
Fadini, Alisia
Perrett, Samuel
Maghlaoui, Karim
Lefèvre, Salomé Bodet
Cordon-Preciado, Violeta
Ferreira, Josie L.
Chukhutsina, Volha U.
Garratt, Douglas
Barnard, Jonathan
Galinis, Gediminas
Glencross, Flo
Morgan, Rhodri M.
Stockton, Sian
Taylor, Ben
Yuan, Letong
Romei, Matthew G.
Lin, Chi-Yun
Marangos, Jon P.
Schmidt, Marius
Chatrchyan, Viktoria
Buckup, Tiago
Morozov, Dmitry
Park, Jaehyun
Park, Sehan
Eom, Intae
Kim, Minseok
Jang, Dogeun
Choi, Hyeongi
Hyun, HyoJung
Park, Gisu
Nango, Eriko
Tanaka, Rie
Owada, Shigeki
Tono, Kensuke
DePonte, Daniel P.
Carbajo, Sergio
Seaberg, Matt
Aquila, Andrew
Boutet, Sebastien
Barty, Anton
Iwata, So
Boxer, Steven G.
Groenhof, Gerrit
van Thor, Jasper J.
author_sort Hutchison, Christopher D. M.
collection PubMed
description The photoisomerization reaction of a fluorescent protein chromophore occurs on the ultrafast timescale. The structural dynamics that result from femtosecond optical excitation have contributions from vibrational and electronic processes and from reaction dynamics that involve the crossing through a conical intersection. The creation and progression of the ultrafast structural dynamics strongly depends on optical and molecular parameters. When using X-ray crystallography as a probe of ultrafast dynamics, the origin of the observed nuclear motions is not known. Now, high-resolution pump–probe X-ray crystallography reveals complex sub-ångström, ultrafast motions and hydrogen-bonding rearrangements in the active site of a fluorescent protein. However, we demonstrate that the measured motions are not part of the photoisomerization reaction but instead arise from impulsively driven coherent vibrational processes in the electronic ground state. A coherent-control experiment using a two-colour and two-pulse optical excitation strongly amplifies the X-ray crystallographic difference density, while it fully depletes the photoisomerization process. A coherent control mechanism was tested and confirmed the wave packets assignment. [Image: see text]
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spelling pubmed-106246172023-11-05 Optical control of ultrafast structural dynamics in a fluorescent protein Hutchison, Christopher D. M. Baxter, James M. Fitzpatrick, Ann Dorlhiac, Gabriel Fadini, Alisia Perrett, Samuel Maghlaoui, Karim Lefèvre, Salomé Bodet Cordon-Preciado, Violeta Ferreira, Josie L. Chukhutsina, Volha U. Garratt, Douglas Barnard, Jonathan Galinis, Gediminas Glencross, Flo Morgan, Rhodri M. Stockton, Sian Taylor, Ben Yuan, Letong Romei, Matthew G. Lin, Chi-Yun Marangos, Jon P. Schmidt, Marius Chatrchyan, Viktoria Buckup, Tiago Morozov, Dmitry Park, Jaehyun Park, Sehan Eom, Intae Kim, Minseok Jang, Dogeun Choi, Hyeongi Hyun, HyoJung Park, Gisu Nango, Eriko Tanaka, Rie Owada, Shigeki Tono, Kensuke DePonte, Daniel P. Carbajo, Sergio Seaberg, Matt Aquila, Andrew Boutet, Sebastien Barty, Anton Iwata, So Boxer, Steven G. Groenhof, Gerrit van Thor, Jasper J. Nat Chem Article The photoisomerization reaction of a fluorescent protein chromophore occurs on the ultrafast timescale. The structural dynamics that result from femtosecond optical excitation have contributions from vibrational and electronic processes and from reaction dynamics that involve the crossing through a conical intersection. The creation and progression of the ultrafast structural dynamics strongly depends on optical and molecular parameters. When using X-ray crystallography as a probe of ultrafast dynamics, the origin of the observed nuclear motions is not known. Now, high-resolution pump–probe X-ray crystallography reveals complex sub-ångström, ultrafast motions and hydrogen-bonding rearrangements in the active site of a fluorescent protein. However, we demonstrate that the measured motions are not part of the photoisomerization reaction but instead arise from impulsively driven coherent vibrational processes in the electronic ground state. A coherent-control experiment using a two-colour and two-pulse optical excitation strongly amplifies the X-ray crystallographic difference density, while it fully depletes the photoisomerization process. A coherent control mechanism was tested and confirmed the wave packets assignment. [Image: see text] Nature Publishing Group UK 2023-08-10 2023 /pmc/articles/PMC10624617/ /pubmed/37563326 http://dx.doi.org/10.1038/s41557-023-01275-1 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Hutchison, Christopher D. M.
Baxter, James M.
Fitzpatrick, Ann
Dorlhiac, Gabriel
Fadini, Alisia
Perrett, Samuel
Maghlaoui, Karim
Lefèvre, Salomé Bodet
Cordon-Preciado, Violeta
Ferreira, Josie L.
Chukhutsina, Volha U.
Garratt, Douglas
Barnard, Jonathan
Galinis, Gediminas
Glencross, Flo
Morgan, Rhodri M.
Stockton, Sian
Taylor, Ben
Yuan, Letong
Romei, Matthew G.
Lin, Chi-Yun
Marangos, Jon P.
Schmidt, Marius
Chatrchyan, Viktoria
Buckup, Tiago
Morozov, Dmitry
Park, Jaehyun
Park, Sehan
Eom, Intae
Kim, Minseok
Jang, Dogeun
Choi, Hyeongi
Hyun, HyoJung
Park, Gisu
Nango, Eriko
Tanaka, Rie
Owada, Shigeki
Tono, Kensuke
DePonte, Daniel P.
Carbajo, Sergio
Seaberg, Matt
Aquila, Andrew
Boutet, Sebastien
Barty, Anton
Iwata, So
Boxer, Steven G.
Groenhof, Gerrit
van Thor, Jasper J.
Optical control of ultrafast structural dynamics in a fluorescent protein
title Optical control of ultrafast structural dynamics in a fluorescent protein
title_full Optical control of ultrafast structural dynamics in a fluorescent protein
title_fullStr Optical control of ultrafast structural dynamics in a fluorescent protein
title_full_unstemmed Optical control of ultrafast structural dynamics in a fluorescent protein
title_short Optical control of ultrafast structural dynamics in a fluorescent protein
title_sort optical control of ultrafast structural dynamics in a fluorescent protein
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10624617/
https://www.ncbi.nlm.nih.gov/pubmed/37563326
http://dx.doi.org/10.1038/s41557-023-01275-1
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