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Optical control of ultrafast structural dynamics in a fluorescent protein
The photoisomerization reaction of a fluorescent protein chromophore occurs on the ultrafast timescale. The structural dynamics that result from femtosecond optical excitation have contributions from vibrational and electronic processes and from reaction dynamics that involve the crossing through a...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10624617/ https://www.ncbi.nlm.nih.gov/pubmed/37563326 http://dx.doi.org/10.1038/s41557-023-01275-1 |
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author | Hutchison, Christopher D. M. Baxter, James M. Fitzpatrick, Ann Dorlhiac, Gabriel Fadini, Alisia Perrett, Samuel Maghlaoui, Karim Lefèvre, Salomé Bodet Cordon-Preciado, Violeta Ferreira, Josie L. Chukhutsina, Volha U. Garratt, Douglas Barnard, Jonathan Galinis, Gediminas Glencross, Flo Morgan, Rhodri M. Stockton, Sian Taylor, Ben Yuan, Letong Romei, Matthew G. Lin, Chi-Yun Marangos, Jon P. Schmidt, Marius Chatrchyan, Viktoria Buckup, Tiago Morozov, Dmitry Park, Jaehyun Park, Sehan Eom, Intae Kim, Minseok Jang, Dogeun Choi, Hyeongi Hyun, HyoJung Park, Gisu Nango, Eriko Tanaka, Rie Owada, Shigeki Tono, Kensuke DePonte, Daniel P. Carbajo, Sergio Seaberg, Matt Aquila, Andrew Boutet, Sebastien Barty, Anton Iwata, So Boxer, Steven G. Groenhof, Gerrit van Thor, Jasper J. |
author_facet | Hutchison, Christopher D. M. Baxter, James M. Fitzpatrick, Ann Dorlhiac, Gabriel Fadini, Alisia Perrett, Samuel Maghlaoui, Karim Lefèvre, Salomé Bodet Cordon-Preciado, Violeta Ferreira, Josie L. Chukhutsina, Volha U. Garratt, Douglas Barnard, Jonathan Galinis, Gediminas Glencross, Flo Morgan, Rhodri M. Stockton, Sian Taylor, Ben Yuan, Letong Romei, Matthew G. Lin, Chi-Yun Marangos, Jon P. Schmidt, Marius Chatrchyan, Viktoria Buckup, Tiago Morozov, Dmitry Park, Jaehyun Park, Sehan Eom, Intae Kim, Minseok Jang, Dogeun Choi, Hyeongi Hyun, HyoJung Park, Gisu Nango, Eriko Tanaka, Rie Owada, Shigeki Tono, Kensuke DePonte, Daniel P. Carbajo, Sergio Seaberg, Matt Aquila, Andrew Boutet, Sebastien Barty, Anton Iwata, So Boxer, Steven G. Groenhof, Gerrit van Thor, Jasper J. |
author_sort | Hutchison, Christopher D. M. |
collection | PubMed |
description | The photoisomerization reaction of a fluorescent protein chromophore occurs on the ultrafast timescale. The structural dynamics that result from femtosecond optical excitation have contributions from vibrational and electronic processes and from reaction dynamics that involve the crossing through a conical intersection. The creation and progression of the ultrafast structural dynamics strongly depends on optical and molecular parameters. When using X-ray crystallography as a probe of ultrafast dynamics, the origin of the observed nuclear motions is not known. Now, high-resolution pump–probe X-ray crystallography reveals complex sub-ångström, ultrafast motions and hydrogen-bonding rearrangements in the active site of a fluorescent protein. However, we demonstrate that the measured motions are not part of the photoisomerization reaction but instead arise from impulsively driven coherent vibrational processes in the electronic ground state. A coherent-control experiment using a two-colour and two-pulse optical excitation strongly amplifies the X-ray crystallographic difference density, while it fully depletes the photoisomerization process. A coherent control mechanism was tested and confirmed the wave packets assignment. [Image: see text] |
format | Online Article Text |
id | pubmed-10624617 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-106246172023-11-05 Optical control of ultrafast structural dynamics in a fluorescent protein Hutchison, Christopher D. M. Baxter, James M. Fitzpatrick, Ann Dorlhiac, Gabriel Fadini, Alisia Perrett, Samuel Maghlaoui, Karim Lefèvre, Salomé Bodet Cordon-Preciado, Violeta Ferreira, Josie L. Chukhutsina, Volha U. Garratt, Douglas Barnard, Jonathan Galinis, Gediminas Glencross, Flo Morgan, Rhodri M. Stockton, Sian Taylor, Ben Yuan, Letong Romei, Matthew G. Lin, Chi-Yun Marangos, Jon P. Schmidt, Marius Chatrchyan, Viktoria Buckup, Tiago Morozov, Dmitry Park, Jaehyun Park, Sehan Eom, Intae Kim, Minseok Jang, Dogeun Choi, Hyeongi Hyun, HyoJung Park, Gisu Nango, Eriko Tanaka, Rie Owada, Shigeki Tono, Kensuke DePonte, Daniel P. Carbajo, Sergio Seaberg, Matt Aquila, Andrew Boutet, Sebastien Barty, Anton Iwata, So Boxer, Steven G. Groenhof, Gerrit van Thor, Jasper J. Nat Chem Article The photoisomerization reaction of a fluorescent protein chromophore occurs on the ultrafast timescale. The structural dynamics that result from femtosecond optical excitation have contributions from vibrational and electronic processes and from reaction dynamics that involve the crossing through a conical intersection. The creation and progression of the ultrafast structural dynamics strongly depends on optical and molecular parameters. When using X-ray crystallography as a probe of ultrafast dynamics, the origin of the observed nuclear motions is not known. Now, high-resolution pump–probe X-ray crystallography reveals complex sub-ångström, ultrafast motions and hydrogen-bonding rearrangements in the active site of a fluorescent protein. However, we demonstrate that the measured motions are not part of the photoisomerization reaction but instead arise from impulsively driven coherent vibrational processes in the electronic ground state. A coherent-control experiment using a two-colour and two-pulse optical excitation strongly amplifies the X-ray crystallographic difference density, while it fully depletes the photoisomerization process. A coherent control mechanism was tested and confirmed the wave packets assignment. [Image: see text] Nature Publishing Group UK 2023-08-10 2023 /pmc/articles/PMC10624617/ /pubmed/37563326 http://dx.doi.org/10.1038/s41557-023-01275-1 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Hutchison, Christopher D. M. Baxter, James M. Fitzpatrick, Ann Dorlhiac, Gabriel Fadini, Alisia Perrett, Samuel Maghlaoui, Karim Lefèvre, Salomé Bodet Cordon-Preciado, Violeta Ferreira, Josie L. Chukhutsina, Volha U. Garratt, Douglas Barnard, Jonathan Galinis, Gediminas Glencross, Flo Morgan, Rhodri M. Stockton, Sian Taylor, Ben Yuan, Letong Romei, Matthew G. Lin, Chi-Yun Marangos, Jon P. Schmidt, Marius Chatrchyan, Viktoria Buckup, Tiago Morozov, Dmitry Park, Jaehyun Park, Sehan Eom, Intae Kim, Minseok Jang, Dogeun Choi, Hyeongi Hyun, HyoJung Park, Gisu Nango, Eriko Tanaka, Rie Owada, Shigeki Tono, Kensuke DePonte, Daniel P. Carbajo, Sergio Seaberg, Matt Aquila, Andrew Boutet, Sebastien Barty, Anton Iwata, So Boxer, Steven G. Groenhof, Gerrit van Thor, Jasper J. Optical control of ultrafast structural dynamics in a fluorescent protein |
title | Optical control of ultrafast structural dynamics in a fluorescent protein |
title_full | Optical control of ultrafast structural dynamics in a fluorescent protein |
title_fullStr | Optical control of ultrafast structural dynamics in a fluorescent protein |
title_full_unstemmed | Optical control of ultrafast structural dynamics in a fluorescent protein |
title_short | Optical control of ultrafast structural dynamics in a fluorescent protein |
title_sort | optical control of ultrafast structural dynamics in a fluorescent protein |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10624617/ https://www.ncbi.nlm.nih.gov/pubmed/37563326 http://dx.doi.org/10.1038/s41557-023-01275-1 |
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