Cargando…

Differential expression of cancer stem cell markers and pro-inflammatory cytokine IL-1β in the oral squamous cell carcinoma and oral submucosal fibrosis

OBJECTIVES: The poor prognosis of oral squamous cell carcinoma (OSCC) is vastly due to late diagnosis. The oral submucosal fibrosis (OSMF) is often unnoticed pathology linked with high risk of malignancy. Recently, we demonstrated that the clinicopathological alterations in OSMF and OSCC patients we...

Descripción completa

Detalles Bibliográficos
Autores principales: Awasthi, Shriddha, Ahmad, Sharique, Gupta, Rahul, Iqbal, Mohammed Shariq, Ahmad, Ausaf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Qassim Uninversity 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10624801/
https://www.ncbi.nlm.nih.gov/pubmed/37929238
_version_ 1785130988435668992
author Awasthi, Shriddha
Ahmad, Sharique
Gupta, Rahul
Iqbal, Mohammed Shariq
Ahmad, Ausaf
author_facet Awasthi, Shriddha
Ahmad, Sharique
Gupta, Rahul
Iqbal, Mohammed Shariq
Ahmad, Ausaf
author_sort Awasthi, Shriddha
collection PubMed
description OBJECTIVES: The poor prognosis of oral squamous cell carcinoma (OSCC) is vastly due to late diagnosis. The oral submucosal fibrosis (OSMF) is often unnoticed pathology linked with high risk of malignancy. Recently, we demonstrated that the clinicopathological alterations in OSMF and OSCC patients were correlated with cancer stem cell (CSCs) markers (CD133 and CD44). However, the parallel alterations of interleukin-1 beta (IL-1β) with CSCs expression are largely unexplored. Thus, we aimed to investigate the relationship between IL-1β alterations and CSC marker expression in both OSMF and OSCC situations. MATERIALS AND METHODS: A total of 135 people have signed up for the study. There were sixty each in OSMF and OSCC groups, as well as 15 healthy controls. Levels of serum IL-1β were examined by ELISA. Immunohistochemistry (IHC) was used to examine the expression of CD133 and CD44. For evaluating differential CSCs expression, IHC scoring (0–4) was utilized. RESULTS: The IHC results showed maximum subjects in the OSMF and OSCC displaying CD44 and CD133 positivity, although the extent of expression in terms of IHC scoring found variable. CD133 and CD44-positive subjects showed increased levels of IL-1β in the OSMF and OSCC group. Nevertheless, the enhancement of IL-1β is more pronounced in the OSCC cases. Further, we observed a direct link of IL-1β levels with IHC scoring. Multivariate regression analysis demonstrated a significant role for CD44 and CD133 positivity in the increase of IL-1β levels. CONCLUSION: We concluded that concurrent simultaneous changes in CSC biomarkers and IL-1β may help with early detection of OSMF and OSCC conditions.
format Online
Article
Text
id pubmed-10624801
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Qassim Uninversity
record_format MEDLINE/PubMed
spelling pubmed-106248012023-11-05 Differential expression of cancer stem cell markers and pro-inflammatory cytokine IL-1β in the oral squamous cell carcinoma and oral submucosal fibrosis Awasthi, Shriddha Ahmad, Sharique Gupta, Rahul Iqbal, Mohammed Shariq Ahmad, Ausaf Int J Health Sci (Qassim) Original Article OBJECTIVES: The poor prognosis of oral squamous cell carcinoma (OSCC) is vastly due to late diagnosis. The oral submucosal fibrosis (OSMF) is often unnoticed pathology linked with high risk of malignancy. Recently, we demonstrated that the clinicopathological alterations in OSMF and OSCC patients were correlated with cancer stem cell (CSCs) markers (CD133 and CD44). However, the parallel alterations of interleukin-1 beta (IL-1β) with CSCs expression are largely unexplored. Thus, we aimed to investigate the relationship between IL-1β alterations and CSC marker expression in both OSMF and OSCC situations. MATERIALS AND METHODS: A total of 135 people have signed up for the study. There were sixty each in OSMF and OSCC groups, as well as 15 healthy controls. Levels of serum IL-1β were examined by ELISA. Immunohistochemistry (IHC) was used to examine the expression of CD133 and CD44. For evaluating differential CSCs expression, IHC scoring (0–4) was utilized. RESULTS: The IHC results showed maximum subjects in the OSMF and OSCC displaying CD44 and CD133 positivity, although the extent of expression in terms of IHC scoring found variable. CD133 and CD44-positive subjects showed increased levels of IL-1β in the OSMF and OSCC group. Nevertheless, the enhancement of IL-1β is more pronounced in the OSCC cases. Further, we observed a direct link of IL-1β levels with IHC scoring. Multivariate regression analysis demonstrated a significant role for CD44 and CD133 positivity in the increase of IL-1β levels. CONCLUSION: We concluded that concurrent simultaneous changes in CSC biomarkers and IL-1β may help with early detection of OSMF and OSCC conditions. Qassim Uninversity 2023 /pmc/articles/PMC10624801/ /pubmed/37929238 Text en Copyright: © International Journal of Health Sciences https://creativecommons.org/licenses/by-nc-sa/3.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Awasthi, Shriddha
Ahmad, Sharique
Gupta, Rahul
Iqbal, Mohammed Shariq
Ahmad, Ausaf
Differential expression of cancer stem cell markers and pro-inflammatory cytokine IL-1β in the oral squamous cell carcinoma and oral submucosal fibrosis
title Differential expression of cancer stem cell markers and pro-inflammatory cytokine IL-1β in the oral squamous cell carcinoma and oral submucosal fibrosis
title_full Differential expression of cancer stem cell markers and pro-inflammatory cytokine IL-1β in the oral squamous cell carcinoma and oral submucosal fibrosis
title_fullStr Differential expression of cancer stem cell markers and pro-inflammatory cytokine IL-1β in the oral squamous cell carcinoma and oral submucosal fibrosis
title_full_unstemmed Differential expression of cancer stem cell markers and pro-inflammatory cytokine IL-1β in the oral squamous cell carcinoma and oral submucosal fibrosis
title_short Differential expression of cancer stem cell markers and pro-inflammatory cytokine IL-1β in the oral squamous cell carcinoma and oral submucosal fibrosis
title_sort differential expression of cancer stem cell markers and pro-inflammatory cytokine il-1β in the oral squamous cell carcinoma and oral submucosal fibrosis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10624801/
https://www.ncbi.nlm.nih.gov/pubmed/37929238
work_keys_str_mv AT awasthishriddha differentialexpressionofcancerstemcellmarkersandproinflammatorycytokineil1bintheoralsquamouscellcarcinomaandoralsubmucosalfibrosis
AT ahmadsharique differentialexpressionofcancerstemcellmarkersandproinflammatorycytokineil1bintheoralsquamouscellcarcinomaandoralsubmucosalfibrosis
AT guptarahul differentialexpressionofcancerstemcellmarkersandproinflammatorycytokineil1bintheoralsquamouscellcarcinomaandoralsubmucosalfibrosis
AT iqbalmohammedshariq differentialexpressionofcancerstemcellmarkersandproinflammatorycytokineil1bintheoralsquamouscellcarcinomaandoralsubmucosalfibrosis
AT ahmadausaf differentialexpressionofcancerstemcellmarkersandproinflammatorycytokineil1bintheoralsquamouscellcarcinomaandoralsubmucosalfibrosis