Transcriptional profiling of early differentiation of primary human mesenchymal stem cells into chondrocytes

Articular cartilage has only very limited regenerative capacities in humans. Tissue engineering techniques for cartilage damage repair are limited in the production of hyaline cartilage. Mesenchymal stem/stromal cells (MSCs) are multipotent stem cells and can be differentiated into mature cartilage...

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Autores principales: Schwarzl, Thomas, Keogh, Andrea, Shaw, Georgina, Krstic, Aleksandar, Clayton, Elizabeth, Higgins, Desmond G., Kolch, Walter, Barry, Frank
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Data Descriptor
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10624874/
https://www.ncbi.nlm.nih.gov/pubmed/37923731
http://dx.doi.org/10.1038/s41597-023-02686-y
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author Schwarzl, Thomas
Keogh, Andrea
Shaw, Georgina
Krstic, Aleksandar
Clayton, Elizabeth
Higgins, Desmond G.
Kolch, Walter
Barry, Frank
author_facet Schwarzl, Thomas
Keogh, Andrea
Shaw, Georgina
Krstic, Aleksandar
Clayton, Elizabeth
Higgins, Desmond G.
Kolch, Walter
Barry, Frank
author_sort Schwarzl, Thomas
collection PubMed
description Articular cartilage has only very limited regenerative capacities in humans. Tissue engineering techniques for cartilage damage repair are limited in the production of hyaline cartilage. Mesenchymal stem/stromal cells (MSCs) are multipotent stem cells and can be differentiated into mature cartilage cells, chondrocytes, which could be used for repairing damaged cartilage. Chondrogenesis is a highly complex, relatively inefficient process lasting over 3 weeks in vitro. Methods: In order to better understand chondrogenic differentiation, especially the commitment phase, we have performed transcriptional profiling of MSC differentiation into chondrocytes from early timepoints starting 15 minutes after induction to 16 hours and fully differentiated chondrocytes at 21 days in triplicates.
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spelling pubmed-106248742023-11-05 Transcriptional profiling of early differentiation of primary human mesenchymal stem cells into chondrocytes Schwarzl, Thomas Keogh, Andrea Shaw, Georgina Krstic, Aleksandar Clayton, Elizabeth Higgins, Desmond G. Kolch, Walter Barry, Frank Sci Data Data Descriptor Articular cartilage has only very limited regenerative capacities in humans. Tissue engineering techniques for cartilage damage repair are limited in the production of hyaline cartilage. Mesenchymal stem/stromal cells (MSCs) are multipotent stem cells and can be differentiated into mature cartilage cells, chondrocytes, which could be used for repairing damaged cartilage. Chondrogenesis is a highly complex, relatively inefficient process lasting over 3 weeks in vitro. Methods: In order to better understand chondrogenic differentiation, especially the commitment phase, we have performed transcriptional profiling of MSC differentiation into chondrocytes from early timepoints starting 15 minutes after induction to 16 hours and fully differentiated chondrocytes at 21 days in triplicates. Nature Publishing Group UK 2023-11-03 /pmc/articles/PMC10624874/ /pubmed/37923731 http://dx.doi.org/10.1038/s41597-023-02686-y Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Data Descriptor
Schwarzl, Thomas
Keogh, Andrea
Shaw, Georgina
Krstic, Aleksandar
Clayton, Elizabeth
Higgins, Desmond G.
Kolch, Walter
Barry, Frank
Transcriptional profiling of early differentiation of primary human mesenchymal stem cells into chondrocytes
title Transcriptional profiling of early differentiation of primary human mesenchymal stem cells into chondrocytes
title_full Transcriptional profiling of early differentiation of primary human mesenchymal stem cells into chondrocytes
title_fullStr Transcriptional profiling of early differentiation of primary human mesenchymal stem cells into chondrocytes
title_full_unstemmed Transcriptional profiling of early differentiation of primary human mesenchymal stem cells into chondrocytes
title_short Transcriptional profiling of early differentiation of primary human mesenchymal stem cells into chondrocytes
title_sort transcriptional profiling of early differentiation of primary human mesenchymal stem cells into chondrocytes
topic Data Descriptor
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10624874/
https://www.ncbi.nlm.nih.gov/pubmed/37923731
http://dx.doi.org/10.1038/s41597-023-02686-y
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