Molecular mechanism of somatic embryogenesis in paeonia ostii ‘Fengdan’ based on transcriptome analysis combined histomorphological observation and metabolite determination

BACKGROUND: Tree peony (Paeonia sect. Moutan DC.) is a famous flower native to China with high ornamental, medicinal, and oil value. However, the low regeneration rate of callus is one of the main constraints for the establishment of a genetic transformation system in tree peony. By histomorphologic...

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Autores principales: Zhang, Wanqing, Zhang, Hongxiao, Zhao, Guodong, Wang, Na, Guo, Lili, Hou, Xiaogai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10625268/
https://www.ncbi.nlm.nih.gov/pubmed/37924006
http://dx.doi.org/10.1186/s12864-023-09730-6
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author Zhang, Wanqing
Zhang, Hongxiao
Zhao, Guodong
Wang, Na
Guo, Lili
Hou, Xiaogai
author_facet Zhang, Wanqing
Zhang, Hongxiao
Zhao, Guodong
Wang, Na
Guo, Lili
Hou, Xiaogai
author_sort Zhang, Wanqing
collection PubMed
description BACKGROUND: Tree peony (Paeonia sect. Moutan DC.) is a famous flower native to China with high ornamental, medicinal, and oil value. However, the low regeneration rate of callus is one of the main constraints for the establishment of a genetic transformation system in tree peony. By histomorphological observation, transcriptomic analysis and metabolite determination, we investigated the molecular mechanism of somatic embryogenesis after the establishment of a culture system and the induction of somatic embryo(SE) formation. RESULTS: We found that SE formation was successfully induced when cotyledons were used as explants. A total of 3185 differentially expressed genes were screened by comparative transcriptomic analysis of embryogenic callus (EC), SE, and non-embryogenic callus (NEC). Compared to NEC, the auxin synthesis-related genes GH3.6 and PCO2 were up-regulated, whereas cytokinin dehydrogenase (CKX6) and CYP450 family genes were down-regulated in somatic embryogenesis. In SE, the auxin content was significantly higher than the cytokinin content. The methyltransferase-related gene S-adenosylmethionine synthase (SAMS) and the flavonoid biosynthesis-related gene (ANS and F3’5’H) were down-regulated in somatic embryogenesis. The determination of flavonoids showed that rhoifolin and hyperoside had the highest content in SE. The results of transcriptome analysis were consistent with the relative expression of 8 candidate genes by quantitative polymerase chain reaction analysis. CONCLUSION: The results revealed that auxin and cytokinin may play a key role in ‘Fengdan’ somatic embryogenesis. The genes related to somatic embryogenesis were revealed, which has partly elucidated the molecular mechanism of somatic embryogenesis in ‘Fengdan’. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-023-09730-6.
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spelling pubmed-106252682023-11-05 Molecular mechanism of somatic embryogenesis in paeonia ostii ‘Fengdan’ based on transcriptome analysis combined histomorphological observation and metabolite determination Zhang, Wanqing Zhang, Hongxiao Zhao, Guodong Wang, Na Guo, Lili Hou, Xiaogai BMC Genomics Research BACKGROUND: Tree peony (Paeonia sect. Moutan DC.) is a famous flower native to China with high ornamental, medicinal, and oil value. However, the low regeneration rate of callus is one of the main constraints for the establishment of a genetic transformation system in tree peony. By histomorphological observation, transcriptomic analysis and metabolite determination, we investigated the molecular mechanism of somatic embryogenesis after the establishment of a culture system and the induction of somatic embryo(SE) formation. RESULTS: We found that SE formation was successfully induced when cotyledons were used as explants. A total of 3185 differentially expressed genes were screened by comparative transcriptomic analysis of embryogenic callus (EC), SE, and non-embryogenic callus (NEC). Compared to NEC, the auxin synthesis-related genes GH3.6 and PCO2 were up-regulated, whereas cytokinin dehydrogenase (CKX6) and CYP450 family genes were down-regulated in somatic embryogenesis. In SE, the auxin content was significantly higher than the cytokinin content. The methyltransferase-related gene S-adenosylmethionine synthase (SAMS) and the flavonoid biosynthesis-related gene (ANS and F3’5’H) were down-regulated in somatic embryogenesis. The determination of flavonoids showed that rhoifolin and hyperoside had the highest content in SE. The results of transcriptome analysis were consistent with the relative expression of 8 candidate genes by quantitative polymerase chain reaction analysis. CONCLUSION: The results revealed that auxin and cytokinin may play a key role in ‘Fengdan’ somatic embryogenesis. The genes related to somatic embryogenesis were revealed, which has partly elucidated the molecular mechanism of somatic embryogenesis in ‘Fengdan’. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-023-09730-6. BioMed Central 2023-11-03 /pmc/articles/PMC10625268/ /pubmed/37924006 http://dx.doi.org/10.1186/s12864-023-09730-6 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Zhang, Wanqing
Zhang, Hongxiao
Zhao, Guodong
Wang, Na
Guo, Lili
Hou, Xiaogai
Molecular mechanism of somatic embryogenesis in paeonia ostii ‘Fengdan’ based on transcriptome analysis combined histomorphological observation and metabolite determination
title Molecular mechanism of somatic embryogenesis in paeonia ostii ‘Fengdan’ based on transcriptome analysis combined histomorphological observation and metabolite determination
title_full Molecular mechanism of somatic embryogenesis in paeonia ostii ‘Fengdan’ based on transcriptome analysis combined histomorphological observation and metabolite determination
title_fullStr Molecular mechanism of somatic embryogenesis in paeonia ostii ‘Fengdan’ based on transcriptome analysis combined histomorphological observation and metabolite determination
title_full_unstemmed Molecular mechanism of somatic embryogenesis in paeonia ostii ‘Fengdan’ based on transcriptome analysis combined histomorphological observation and metabolite determination
title_short Molecular mechanism of somatic embryogenesis in paeonia ostii ‘Fengdan’ based on transcriptome analysis combined histomorphological observation and metabolite determination
title_sort molecular mechanism of somatic embryogenesis in paeonia ostii ‘fengdan’ based on transcriptome analysis combined histomorphological observation and metabolite determination
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10625268/
https://www.ncbi.nlm.nih.gov/pubmed/37924006
http://dx.doi.org/10.1186/s12864-023-09730-6
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