Cargando…
The extracellular polysaccharide inhibit porcine epidemic diarrhea virus with extract and gene editing Lacticaseibacillus
Lacticaseibacillus is one of the predominant microorganisms in gut from human and animal, and the lacticaseibacillus have effective applications against the viral diarrhea of piglets in the farm. However, the function and the concrete cell single pathways of the active ingredient from lacticaseibaci...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10625274/ https://www.ncbi.nlm.nih.gov/pubmed/37924089 http://dx.doi.org/10.1186/s12934-023-02226-8 |
_version_ | 1785131097512738816 |
---|---|
author | Chen, Shaojun Nai, Zida Qin, Ziliang Li, Gang He, Xinmiao Wang, Wentao Tian, Yaguang Liu, Di Jiang, Xinpeng |
author_facet | Chen, Shaojun Nai, Zida Qin, Ziliang Li, Gang He, Xinmiao Wang, Wentao Tian, Yaguang Liu, Di Jiang, Xinpeng |
author_sort | Chen, Shaojun |
collection | PubMed |
description | Lacticaseibacillus is one of the predominant microorganisms in gut from human and animal, and the lacticaseibacillus have effective applications against the viral diarrhea of piglets in the farm. However, the function and the concrete cell single pathways of the active ingredient from lacticaseibacillus was not clear within anti-infection in the postbiotics research. Here, we compared the biological function of extracellular polysaccharides (EPS) purified from lacticaseibacillus casei (L. casei) and gene editing lacticaseibacillus casei with the CRISPER-Cas9 technology, which were with the ability of antioxidation and anti-inflammation, and the EPS could also inhibit the ROS production within the Porcine Small Intestinal Epithelial Cells-J2 (IPEC-J2). Interestingly, we found that both of EPS and genome editing lacticaseibacillus casei could specifically target the IFN-λ expression in the IPEC-J2, which was beneficial against the PEDV infection in the virus replication and production with the qRT-PCR and indirect immunofluorescence methods. Finally, the STAT3 cell single pathway was stimulated to transcribe IFN-λ with the EPS to elucidate the detailed mechanism of activating type III IFN signals receptor of IL-10R2, which play the function between anti-inflammation and anti-virus in the PEDV infection. Taken together, our research linked a postbiotics of EPS with the antiviral infection of PEDV, which suggest that the lacticaseibacillus itself still have displayed the potential immunomodulatory activities, and highlight the immunomodulatory potential of EPS-producing microbes. GRAPHICAL ABSTRACT: [Image: see text] |
format | Online Article Text |
id | pubmed-10625274 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-106252742023-11-05 The extracellular polysaccharide inhibit porcine epidemic diarrhea virus with extract and gene editing Lacticaseibacillus Chen, Shaojun Nai, Zida Qin, Ziliang Li, Gang He, Xinmiao Wang, Wentao Tian, Yaguang Liu, Di Jiang, Xinpeng Microb Cell Fact Research Lacticaseibacillus is one of the predominant microorganisms in gut from human and animal, and the lacticaseibacillus have effective applications against the viral diarrhea of piglets in the farm. However, the function and the concrete cell single pathways of the active ingredient from lacticaseibacillus was not clear within anti-infection in the postbiotics research. Here, we compared the biological function of extracellular polysaccharides (EPS) purified from lacticaseibacillus casei (L. casei) and gene editing lacticaseibacillus casei with the CRISPER-Cas9 technology, which were with the ability of antioxidation and anti-inflammation, and the EPS could also inhibit the ROS production within the Porcine Small Intestinal Epithelial Cells-J2 (IPEC-J2). Interestingly, we found that both of EPS and genome editing lacticaseibacillus casei could specifically target the IFN-λ expression in the IPEC-J2, which was beneficial against the PEDV infection in the virus replication and production with the qRT-PCR and indirect immunofluorescence methods. Finally, the STAT3 cell single pathway was stimulated to transcribe IFN-λ with the EPS to elucidate the detailed mechanism of activating type III IFN signals receptor of IL-10R2, which play the function between anti-inflammation and anti-virus in the PEDV infection. Taken together, our research linked a postbiotics of EPS with the antiviral infection of PEDV, which suggest that the lacticaseibacillus itself still have displayed the potential immunomodulatory activities, and highlight the immunomodulatory potential of EPS-producing microbes. GRAPHICAL ABSTRACT: [Image: see text] BioMed Central 2023-11-04 /pmc/articles/PMC10625274/ /pubmed/37924089 http://dx.doi.org/10.1186/s12934-023-02226-8 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Chen, Shaojun Nai, Zida Qin, Ziliang Li, Gang He, Xinmiao Wang, Wentao Tian, Yaguang Liu, Di Jiang, Xinpeng The extracellular polysaccharide inhibit porcine epidemic diarrhea virus with extract and gene editing Lacticaseibacillus |
title | The extracellular polysaccharide inhibit porcine epidemic diarrhea virus with extract and gene editing Lacticaseibacillus |
title_full | The extracellular polysaccharide inhibit porcine epidemic diarrhea virus with extract and gene editing Lacticaseibacillus |
title_fullStr | The extracellular polysaccharide inhibit porcine epidemic diarrhea virus with extract and gene editing Lacticaseibacillus |
title_full_unstemmed | The extracellular polysaccharide inhibit porcine epidemic diarrhea virus with extract and gene editing Lacticaseibacillus |
title_short | The extracellular polysaccharide inhibit porcine epidemic diarrhea virus with extract and gene editing Lacticaseibacillus |
title_sort | extracellular polysaccharide inhibit porcine epidemic diarrhea virus with extract and gene editing lacticaseibacillus |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10625274/ https://www.ncbi.nlm.nih.gov/pubmed/37924089 http://dx.doi.org/10.1186/s12934-023-02226-8 |
work_keys_str_mv | AT chenshaojun theextracellularpolysaccharideinhibitporcineepidemicdiarrheaviruswithextractandgeneeditinglacticaseibacillus AT naizida theextracellularpolysaccharideinhibitporcineepidemicdiarrheaviruswithextractandgeneeditinglacticaseibacillus AT qinziliang theextracellularpolysaccharideinhibitporcineepidemicdiarrheaviruswithextractandgeneeditinglacticaseibacillus AT ligang theextracellularpolysaccharideinhibitporcineepidemicdiarrheaviruswithextractandgeneeditinglacticaseibacillus AT hexinmiao theextracellularpolysaccharideinhibitporcineepidemicdiarrheaviruswithextractandgeneeditinglacticaseibacillus AT wangwentao theextracellularpolysaccharideinhibitporcineepidemicdiarrheaviruswithextractandgeneeditinglacticaseibacillus AT tianyaguang theextracellularpolysaccharideinhibitporcineepidemicdiarrheaviruswithextractandgeneeditinglacticaseibacillus AT liudi theextracellularpolysaccharideinhibitporcineepidemicdiarrheaviruswithextractandgeneeditinglacticaseibacillus AT jiangxinpeng theextracellularpolysaccharideinhibitporcineepidemicdiarrheaviruswithextractandgeneeditinglacticaseibacillus AT chenshaojun extracellularpolysaccharideinhibitporcineepidemicdiarrheaviruswithextractandgeneeditinglacticaseibacillus AT naizida extracellularpolysaccharideinhibitporcineepidemicdiarrheaviruswithextractandgeneeditinglacticaseibacillus AT qinziliang extracellularpolysaccharideinhibitporcineepidemicdiarrheaviruswithextractandgeneeditinglacticaseibacillus AT ligang extracellularpolysaccharideinhibitporcineepidemicdiarrheaviruswithextractandgeneeditinglacticaseibacillus AT hexinmiao extracellularpolysaccharideinhibitporcineepidemicdiarrheaviruswithextractandgeneeditinglacticaseibacillus AT wangwentao extracellularpolysaccharideinhibitporcineepidemicdiarrheaviruswithextractandgeneeditinglacticaseibacillus AT tianyaguang extracellularpolysaccharideinhibitporcineepidemicdiarrheaviruswithextractandgeneeditinglacticaseibacillus AT liudi extracellularpolysaccharideinhibitporcineepidemicdiarrheaviruswithextractandgeneeditinglacticaseibacillus AT jiangxinpeng extracellularpolysaccharideinhibitporcineepidemicdiarrheaviruswithextractandgeneeditinglacticaseibacillus |