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Genomic potential and physiological characteristics of C1 metabolism in novel acetogenic bacteria

Acetogenic bacteria can utilize C1 compounds, such as carbon monoxide (CO), formate, and methanol, via the Wood-Ljungdahl pathway (WLP) to produce biofuels and biochemicals. Two novel acetogenic bacteria of the family Eubacteriaceae ES2 and ES3 were isolated from Eulsukdo, a delta island in South Ko...

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Autores principales: Yu, Jihyun, Park, Mi-Jeong, Lee, Joungmin, Kwon, Soo Jae, Lim, Jae Kyu, Lee, Hyun Sook, Kang, Sung Gyun, Lee, Jung-Hyun, Kwon, Kae Kyoung, Kim, Yun Jae
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10625859/
https://www.ncbi.nlm.nih.gov/pubmed/37933250
http://dx.doi.org/10.3389/fmicb.2023.1279544
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author Yu, Jihyun
Park, Mi-Jeong
Lee, Joungmin
Kwon, Soo Jae
Lim, Jae Kyu
Lee, Hyun Sook
Kang, Sung Gyun
Lee, Jung-Hyun
Kwon, Kae Kyoung
Kim, Yun Jae
author_facet Yu, Jihyun
Park, Mi-Jeong
Lee, Joungmin
Kwon, Soo Jae
Lim, Jae Kyu
Lee, Hyun Sook
Kang, Sung Gyun
Lee, Jung-Hyun
Kwon, Kae Kyoung
Kim, Yun Jae
author_sort Yu, Jihyun
collection PubMed
description Acetogenic bacteria can utilize C1 compounds, such as carbon monoxide (CO), formate, and methanol, via the Wood-Ljungdahl pathway (WLP) to produce biofuels and biochemicals. Two novel acetogenic bacteria of the family Eubacteriaceae ES2 and ES3 were isolated from Eulsukdo, a delta island in South Korea. We conducted whole genome sequencing of the ES strains and comparative genome analysis on the core clusters of WLP with Acetobacterium woodii DSM1030(T) and Eubacterium limosum ATCC8486(T). The methyl-branch cluster included a formate transporter and duplicates or triplicates copies of the fhs gene, which encodes formyl-tetrahydrofolate synthetase. The formate dehydrogenase cluster did not include the hydrogenase gene, which might be replaced by a functional complex with a separate electron bifurcating hydrogenase (HytABCDE). Additionally, duplicated copies of the acsB gene, encoding acetyl-CoA synthase, are located within or close to the carbonyl-branch cluster. The serum bottle culture showed that ES strains can utilize a diverse range of C1 compounds, including CO, formate, and methanol, as well as CO(2). Notably, ES2 exhibited remarkable resistance to high concentrations of C1 substrates, such as 100% CO (200 kPa), 700 mM formate, and 500 mM methanol. Moreover, ES2 demonstrated remarkable growth rates under 50% CO (0.45 h(−1)) and 200 mM formate (0.34 h(−1)). These growth rates are comparable to or surpassing those previously reported in other acetogenic bacteria. Our study introduces novel acetogenic ES strains and describes their genetic and physiological characteristics, which can be utilized in C1-based biomanufacturing.
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spelling pubmed-106258592023-11-06 Genomic potential and physiological characteristics of C1 metabolism in novel acetogenic bacteria Yu, Jihyun Park, Mi-Jeong Lee, Joungmin Kwon, Soo Jae Lim, Jae Kyu Lee, Hyun Sook Kang, Sung Gyun Lee, Jung-Hyun Kwon, Kae Kyoung Kim, Yun Jae Front Microbiol Microbiology Acetogenic bacteria can utilize C1 compounds, such as carbon monoxide (CO), formate, and methanol, via the Wood-Ljungdahl pathway (WLP) to produce biofuels and biochemicals. Two novel acetogenic bacteria of the family Eubacteriaceae ES2 and ES3 were isolated from Eulsukdo, a delta island in South Korea. We conducted whole genome sequencing of the ES strains and comparative genome analysis on the core clusters of WLP with Acetobacterium woodii DSM1030(T) and Eubacterium limosum ATCC8486(T). The methyl-branch cluster included a formate transporter and duplicates or triplicates copies of the fhs gene, which encodes formyl-tetrahydrofolate synthetase. The formate dehydrogenase cluster did not include the hydrogenase gene, which might be replaced by a functional complex with a separate electron bifurcating hydrogenase (HytABCDE). Additionally, duplicated copies of the acsB gene, encoding acetyl-CoA synthase, are located within or close to the carbonyl-branch cluster. The serum bottle culture showed that ES strains can utilize a diverse range of C1 compounds, including CO, formate, and methanol, as well as CO(2). Notably, ES2 exhibited remarkable resistance to high concentrations of C1 substrates, such as 100% CO (200 kPa), 700 mM formate, and 500 mM methanol. Moreover, ES2 demonstrated remarkable growth rates under 50% CO (0.45 h(−1)) and 200 mM formate (0.34 h(−1)). These growth rates are comparable to or surpassing those previously reported in other acetogenic bacteria. Our study introduces novel acetogenic ES strains and describes their genetic and physiological characteristics, which can be utilized in C1-based biomanufacturing. Frontiers Media S.A. 2023-10-19 /pmc/articles/PMC10625859/ /pubmed/37933250 http://dx.doi.org/10.3389/fmicb.2023.1279544 Text en Copyright © 2023 Yu, Park, Lee, Kwon, Lim, Lee, Kang, Lee, Kwon and Kim. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Yu, Jihyun
Park, Mi-Jeong
Lee, Joungmin
Kwon, Soo Jae
Lim, Jae Kyu
Lee, Hyun Sook
Kang, Sung Gyun
Lee, Jung-Hyun
Kwon, Kae Kyoung
Kim, Yun Jae
Genomic potential and physiological characteristics of C1 metabolism in novel acetogenic bacteria
title Genomic potential and physiological characteristics of C1 metabolism in novel acetogenic bacteria
title_full Genomic potential and physiological characteristics of C1 metabolism in novel acetogenic bacteria
title_fullStr Genomic potential and physiological characteristics of C1 metabolism in novel acetogenic bacteria
title_full_unstemmed Genomic potential and physiological characteristics of C1 metabolism in novel acetogenic bacteria
title_short Genomic potential and physiological characteristics of C1 metabolism in novel acetogenic bacteria
title_sort genomic potential and physiological characteristics of c1 metabolism in novel acetogenic bacteria
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10625859/
https://www.ncbi.nlm.nih.gov/pubmed/37933250
http://dx.doi.org/10.3389/fmicb.2023.1279544
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