Barcoding intracellular reverse transcription enables high-throughput phenotype-coupled T cell receptor analyses

Assays linking cellular phenotypes with T cell or B cell antigen receptor sequences are crucial for characterizing adaptive immune responses. Existing methodologies are limited by low sample throughput and high cost. Here, we present INtraCEllular Reverse Transcription with Sorting and sequencing (I...

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Detalles Bibliográficos
Autores principales: Jayaraman, Sahana, Montagne, Janelle M., Nirschl, Thomas R., Marcisak, Emily, Johnson, Jeanette, Huff, Amanda, Hsiao, Meng-Hsuan, Nauroth, Julie, Heumann, Thatcher, Zarif, Jelani C., Jaffee, Elizabeth M., Azad, Nilo, Fertig, Elana J., Zaidi, Neeha, Larman, H. Benjamin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10626196/
https://www.ncbi.nlm.nih.gov/pubmed/37776855
http://dx.doi.org/10.1016/j.crmeth.2023.100600

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