Mind your tag in single-molecule measurements

In this issue of Cell Reports Methods, Molina and colleagues use in vitro single-molecule DNA flow-stretching to demonstrate the severe effects of appending a short lysine-cysteine-lysine (KCK) tag on the Bacillus subtilis ParB protein. This assay could be further utilized to evaluate the impact of...

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Detalles Bibliográficos
Autores principales: Merino Urteaga, Raquel, Ha, Taekjip
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10626269/
https://www.ncbi.nlm.nih.gov/pubmed/37883922
http://dx.doi.org/10.1016/j.crmeth.2023.100623
Descripción
Sumario:In this issue of Cell Reports Methods, Molina and colleagues use in vitro single-molecule DNA flow-stretching to demonstrate the severe effects of appending a short lysine-cysteine-lysine (KCK) tag on the Bacillus subtilis ParB protein. This assay could be further utilized to evaluate the impact of other tags on DNA-binding proteins.

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