Mind your tag in single-molecule measurements

In this issue of Cell Reports Methods, Molina and colleagues use in vitro single-molecule DNA flow-stretching to demonstrate the severe effects of appending a short lysine-cysteine-lysine (KCK) tag on the Bacillus subtilis ParB protein. This assay could be further utilized to evaluate the impact of...

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Detalles Bibliográficos
Autores principales: Merino Urteaga, Raquel, Ha, Taekjip
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10626269/
https://www.ncbi.nlm.nih.gov/pubmed/37883922
http://dx.doi.org/10.1016/j.crmeth.2023.100623
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author Merino Urteaga, Raquel
Ha, Taekjip
author_facet Merino Urteaga, Raquel
Ha, Taekjip
author_sort Merino Urteaga, Raquel
collection PubMed
description In this issue of Cell Reports Methods, Molina and colleagues use in vitro single-molecule DNA flow-stretching to demonstrate the severe effects of appending a short lysine-cysteine-lysine (KCK) tag on the Bacillus subtilis ParB protein. This assay could be further utilized to evaluate the impact of other tags on DNA-binding proteins.
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spelling pubmed-106262692023-11-07 Mind your tag in single-molecule measurements Merino Urteaga, Raquel Ha, Taekjip Cell Rep Methods Preview In this issue of Cell Reports Methods, Molina and colleagues use in vitro single-molecule DNA flow-stretching to demonstrate the severe effects of appending a short lysine-cysteine-lysine (KCK) tag on the Bacillus subtilis ParB protein. This assay could be further utilized to evaluate the impact of other tags on DNA-binding proteins. Elsevier 2023-10-25 /pmc/articles/PMC10626269/ /pubmed/37883922 http://dx.doi.org/10.1016/j.crmeth.2023.100623 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Preview
Merino Urteaga, Raquel
Ha, Taekjip
Mind your tag in single-molecule measurements
title Mind your tag in single-molecule measurements
title_full Mind your tag in single-molecule measurements
title_fullStr Mind your tag in single-molecule measurements
title_full_unstemmed Mind your tag in single-molecule measurements
title_short Mind your tag in single-molecule measurements
title_sort mind your tag in single-molecule measurements
topic Preview
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10626269/
https://www.ncbi.nlm.nih.gov/pubmed/37883922
http://dx.doi.org/10.1016/j.crmeth.2023.100623
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