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Genetic dissection of morphological variation between cauliflower and a rapid cycling Brassica oleracea line

To improve resolution to small genomic regions and sensitivity to small-effect loci in the identification of genetic factors conferring the enlarged inflorescence and other traits of cauliflower while also expediting further genetic dissection, 104 near-isogenic introgression lines (NIILs) covering...

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Autores principales: Shuang, Lan Shuan, Cuevas, Hugo, Lemke, Cornelia, Kim, Changsoo, Shehzad, Tariq, Paterson, Andrew H
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10627287/
https://www.ncbi.nlm.nih.gov/pubmed/37506262
http://dx.doi.org/10.1093/g3journal/jkad163
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author Shuang, Lan Shuan
Cuevas, Hugo
Lemke, Cornelia
Kim, Changsoo
Shehzad, Tariq
Paterson, Andrew H
author_facet Shuang, Lan Shuan
Cuevas, Hugo
Lemke, Cornelia
Kim, Changsoo
Shehzad, Tariq
Paterson, Andrew H
author_sort Shuang, Lan Shuan
collection PubMed
description To improve resolution to small genomic regions and sensitivity to small-effect loci in the identification of genetic factors conferring the enlarged inflorescence and other traits of cauliflower while also expediting further genetic dissection, 104 near-isogenic introgression lines (NIILs) covering 78.56% of the cauliflower genome, were selected from an advanced backcross population using cauliflower [Brassica oleracea var. botrytis L., mutant for Orange gene (ORG)] as the donor parent and a rapid cycling line (TO1434) as recurrent parent. Subsets of the advanced backcross population and NIILs were planted in the field for 8 seasons, finding 141 marker-trait associations for 15 leaf-, stem-, and flower-traits. Exemplifying the usefulness of these lines, we delineated the previously known flower color gene to a 4.5 MB interval on C3; a gene for small plant size to a 3.4 MB region on C8; and a gene for large plant size and flowering time to a 6.1 MB region on C9. This approach unmasked closely linked QTL alleles with opposing effects (on chr. 8) and revealed both alleles with expected phenotypic effects and effects opposite the parental phenotypes. Selected B. oleracea NIILs with short generation time add new value to widely used research and teaching materials.
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spelling pubmed-106272872023-11-07 Genetic dissection of morphological variation between cauliflower and a rapid cycling Brassica oleracea line Shuang, Lan Shuan Cuevas, Hugo Lemke, Cornelia Kim, Changsoo Shehzad, Tariq Paterson, Andrew H G3 (Bethesda) Plant Genetics and Genomics To improve resolution to small genomic regions and sensitivity to small-effect loci in the identification of genetic factors conferring the enlarged inflorescence and other traits of cauliflower while also expediting further genetic dissection, 104 near-isogenic introgression lines (NIILs) covering 78.56% of the cauliflower genome, were selected from an advanced backcross population using cauliflower [Brassica oleracea var. botrytis L., mutant for Orange gene (ORG)] as the donor parent and a rapid cycling line (TO1434) as recurrent parent. Subsets of the advanced backcross population and NIILs were planted in the field for 8 seasons, finding 141 marker-trait associations for 15 leaf-, stem-, and flower-traits. Exemplifying the usefulness of these lines, we delineated the previously known flower color gene to a 4.5 MB interval on C3; a gene for small plant size to a 3.4 MB region on C8; and a gene for large plant size and flowering time to a 6.1 MB region on C9. This approach unmasked closely linked QTL alleles with opposing effects (on chr. 8) and revealed both alleles with expected phenotypic effects and effects opposite the parental phenotypes. Selected B. oleracea NIILs with short generation time add new value to widely used research and teaching materials. Oxford University Press 2023-08-28 /pmc/articles/PMC10627287/ /pubmed/37506262 http://dx.doi.org/10.1093/g3journal/jkad163 Text en © The Author(s) 2023. Published by Oxford University Press on behalf of The Genetics Society of America. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Plant Genetics and Genomics
Shuang, Lan Shuan
Cuevas, Hugo
Lemke, Cornelia
Kim, Changsoo
Shehzad, Tariq
Paterson, Andrew H
Genetic dissection of morphological variation between cauliflower and a rapid cycling Brassica oleracea line
title Genetic dissection of morphological variation between cauliflower and a rapid cycling Brassica oleracea line
title_full Genetic dissection of morphological variation between cauliflower and a rapid cycling Brassica oleracea line
title_fullStr Genetic dissection of morphological variation between cauliflower and a rapid cycling Brassica oleracea line
title_full_unstemmed Genetic dissection of morphological variation between cauliflower and a rapid cycling Brassica oleracea line
title_short Genetic dissection of morphological variation between cauliflower and a rapid cycling Brassica oleracea line
title_sort genetic dissection of morphological variation between cauliflower and a rapid cycling brassica oleracea line
topic Plant Genetics and Genomics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10627287/
https://www.ncbi.nlm.nih.gov/pubmed/37506262
http://dx.doi.org/10.1093/g3journal/jkad163
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