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Genetic dissection of morphological variation between cauliflower and a rapid cycling Brassica oleracea line
To improve resolution to small genomic regions and sensitivity to small-effect loci in the identification of genetic factors conferring the enlarged inflorescence and other traits of cauliflower while also expediting further genetic dissection, 104 near-isogenic introgression lines (NIILs) covering...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10627287/ https://www.ncbi.nlm.nih.gov/pubmed/37506262 http://dx.doi.org/10.1093/g3journal/jkad163 |
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author | Shuang, Lan Shuan Cuevas, Hugo Lemke, Cornelia Kim, Changsoo Shehzad, Tariq Paterson, Andrew H |
author_facet | Shuang, Lan Shuan Cuevas, Hugo Lemke, Cornelia Kim, Changsoo Shehzad, Tariq Paterson, Andrew H |
author_sort | Shuang, Lan Shuan |
collection | PubMed |
description | To improve resolution to small genomic regions and sensitivity to small-effect loci in the identification of genetic factors conferring the enlarged inflorescence and other traits of cauliflower while also expediting further genetic dissection, 104 near-isogenic introgression lines (NIILs) covering 78.56% of the cauliflower genome, were selected from an advanced backcross population using cauliflower [Brassica oleracea var. botrytis L., mutant for Orange gene (ORG)] as the donor parent and a rapid cycling line (TO1434) as recurrent parent. Subsets of the advanced backcross population and NIILs were planted in the field for 8 seasons, finding 141 marker-trait associations for 15 leaf-, stem-, and flower-traits. Exemplifying the usefulness of these lines, we delineated the previously known flower color gene to a 4.5 MB interval on C3; a gene for small plant size to a 3.4 MB region on C8; and a gene for large plant size and flowering time to a 6.1 MB region on C9. This approach unmasked closely linked QTL alleles with opposing effects (on chr. 8) and revealed both alleles with expected phenotypic effects and effects opposite the parental phenotypes. Selected B. oleracea NIILs with short generation time add new value to widely used research and teaching materials. |
format | Online Article Text |
id | pubmed-10627287 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-106272872023-11-07 Genetic dissection of morphological variation between cauliflower and a rapid cycling Brassica oleracea line Shuang, Lan Shuan Cuevas, Hugo Lemke, Cornelia Kim, Changsoo Shehzad, Tariq Paterson, Andrew H G3 (Bethesda) Plant Genetics and Genomics To improve resolution to small genomic regions and sensitivity to small-effect loci in the identification of genetic factors conferring the enlarged inflorescence and other traits of cauliflower while also expediting further genetic dissection, 104 near-isogenic introgression lines (NIILs) covering 78.56% of the cauliflower genome, were selected from an advanced backcross population using cauliflower [Brassica oleracea var. botrytis L., mutant for Orange gene (ORG)] as the donor parent and a rapid cycling line (TO1434) as recurrent parent. Subsets of the advanced backcross population and NIILs were planted in the field for 8 seasons, finding 141 marker-trait associations for 15 leaf-, stem-, and flower-traits. Exemplifying the usefulness of these lines, we delineated the previously known flower color gene to a 4.5 MB interval on C3; a gene for small plant size to a 3.4 MB region on C8; and a gene for large plant size and flowering time to a 6.1 MB region on C9. This approach unmasked closely linked QTL alleles with opposing effects (on chr. 8) and revealed both alleles with expected phenotypic effects and effects opposite the parental phenotypes. Selected B. oleracea NIILs with short generation time add new value to widely used research and teaching materials. Oxford University Press 2023-08-28 /pmc/articles/PMC10627287/ /pubmed/37506262 http://dx.doi.org/10.1093/g3journal/jkad163 Text en © The Author(s) 2023. Published by Oxford University Press on behalf of The Genetics Society of America. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Plant Genetics and Genomics Shuang, Lan Shuan Cuevas, Hugo Lemke, Cornelia Kim, Changsoo Shehzad, Tariq Paterson, Andrew H Genetic dissection of morphological variation between cauliflower and a rapid cycling Brassica oleracea line |
title | Genetic dissection of morphological variation between cauliflower and a rapid cycling Brassica oleracea line |
title_full | Genetic dissection of morphological variation between cauliflower and a rapid cycling Brassica oleracea line |
title_fullStr | Genetic dissection of morphological variation between cauliflower and a rapid cycling Brassica oleracea line |
title_full_unstemmed | Genetic dissection of morphological variation between cauliflower and a rapid cycling Brassica oleracea line |
title_short | Genetic dissection of morphological variation between cauliflower and a rapid cycling Brassica oleracea line |
title_sort | genetic dissection of morphological variation between cauliflower and a rapid cycling brassica oleracea line |
topic | Plant Genetics and Genomics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10627287/ https://www.ncbi.nlm.nih.gov/pubmed/37506262 http://dx.doi.org/10.1093/g3journal/jkad163 |
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