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Single-tube multiplex real-time PCR with EvaGreen and high-resolution melting analysis for diagnosis of α(0)-thalassemia--(SEA),--(THAI), and--(CR) type deletions

Regions with a high prevalence of α-thalassemia (α-thal) require simple, rapid, and accurate tests for carrier screening and prenatal diagnosis. Diagnosis of multiple deletions in a single tube is necessary to clearly identify individuals with α(0)-thalassemia in the routine setting, especially in a...

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Detalles Bibliográficos
Autores principales: Ruengdit, Chedtapak, Punyamung, Manoo, Intasai, Nutjeera, Pornprasert, Sakorn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10627449/
https://www.ncbi.nlm.nih.gov/pubmed/37930985
http://dx.doi.org/10.1371/journal.pone.0293838

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